EPCAM (NM_002354) Human Untagged Clone

CAT#: SC118692

EPCAM (untagged)-Human epithelial cell adhesion molecule (EPCAM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPCAM
• Synonyms: DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118692 sequence for NM_002354 edited (data generated by NextGen Sequencing)
  ATGGCGCCCCCGCAGGTCCTCGCGTTCGGGCTTCTGCTTGCCGCGGCGACGGCGACTTTT
  GCCGCAGCTCAGGAAGAATGTGTCTGTGAAAACTACAAGCTGGCCGTAAACTGCTTTGTG
  AATAATAATCGTCAATGCCAGTGTACTTCAGTTGGTGCACAAAATACTGTCATTTGCTCA
  AAGCTGGCTGCCAAATGTTTGGTGATGAAGGCAGAAATGAATGGCTCAAAACTTGGGAGA
  AGAGCAAAACCTGAAGGGGCCCTCCAGAACAATGATGGGCTTTATGATCCTGACTGCGAT
  GAGAGCGGGCTCTTTAAGGCCAAGCAGTGCAACGGCACCTCCACGTGCTGGTGTGTGAAC
  ACTGCTGGGGTCAGAAGAACAGACAAGGACACTGAAATAACCTGCTCTGAGCGAGTGAGA
  ACCTACTGGATCATCATTGAACTAAAACACAAAGCAAGAGAAAAACCTTATGATAGTAAA
  AGTTTGCGGACTGCACTTCAGAAGGAGATCACAACGCGTTATCAACTGGATCCAAAATTT
  ATCACGAGTATTTTGTATGAGAATAATGTTATCACTATTGATCTGGTTCAAAATTCTTCT
  CAAAAAACTCAGAATGATGTGGACATAGCTGATGTGGCTTATTATTTTGAAAAAGATGTT
  AAAGGTGAATCCTTGTTTCATTCTAAGAAAATGGACCTGACAGTAAATGGGGAACAACTG
  GATCTGGATCCTGGTCAAACTTTAATTTATTATGTTGATGAAAAAGCACCTGAATTCTCA
  ATGCAGGGTCTAAAAGCTGGTGTTATTGCTGTTATTGTGGTTGTGGTGATAGCAGTTGTT
  GCTGGAATTGTTGTGCTGGTTATTTCCAGAAAGAAGAGAATGGCAAAGTATGAGAAGGCT
  GAGATAAAGGAGATGGGTGAGATGCATAGGGAACTCAATGCATAA
  
  Clone variation with respect to NM_002354.2
  344 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002354 unedited
  GTTGGGATTTGTATCCGACTTATATAGGGCGGCCGCGATTCGGCACGAGGCTTCGGCGAG
  CGAGCACCTTCGACGCGGTCCGGGGACCCCCTCGTCGCTGTCCTCCCGACGCGGACCCGC
  GTGCCCCAGGCCTCGCGCTGCCCGGCCGGCTCCTCGTGTCCCACTCCCGGCGCACGCCCT
  CCCGCGAGTCCCGGGCCCCTCCCGCGCCCCTCTTCTCGGCGCGCGCGCAGCATGGCGCCC
  CCGCAGGTCCTCGCGTTCGGGCTTCTGCTTGCCGCGGCGACGGCGACTTTTGCCGCAGCT
  CAGGAAGAATGTGTCTGTGAAAACTACAAGCTGGCCGTAAACTGCTTTGTGAATAATAAT
  CGTCAATGCCAGTGTACTTCAGTTGGTGCACAAAATACTGTCATTTGCTCAAAGCTGGCT
  GCCAAATGTTTGGTGATGAAGGCAGAAATGAATGGCTCAAAACTTGGGAGAAGAGCAAAA
  CCTGAAGGGGCCCTCCAGAACAATGATGGGCTTTATGATCCTGACTGCGATGAGAGCGGG
  CTCTTTAAGGCCAAGCAGTGCAACGGCACCTCCACGTGCTGGTGTGTGAACACTGCTGGG
  GTCAGAAGAACAGACCAGGACACTGAAATAACCTGCTCTGAGCGAGTGAGAACCTACTGG
  ATCATCATTGAACTAAAACACAAAGCAAGAGAAAAACCTTATGATAGTAAAAGTTTGCGG
  ACTGCACTTCAGAAAGGAGATCACACGCGTTATCAACTGGATCCAAATTTATCACGAGTA
  TTTTGTATGAGAAATATGTTATCACTATTGATCTGGGTCANAATTCTTCTCAAAAACTCA
  GATGATGTGGCATAGCTGATGTGGCTTATTATTTTGAAAAGATGTTAAGGTGAATCTGNT
  TCATTCTAGAATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002354 unedited
  GTACGCGGCCGCTCTCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCAGTTTTTCAGATTTT
  ACTGTGGAAGCATATTTAATGCACACATCTTGAATGTTACACATAAATAATTTTAACGAT
  GGAGTCCAAGTTCTGGATTTTACATTAGATCTGCATATATAAGACACTTGTGGTCAAATT
  TCAAGATTGGTAAAGCCAGTTTCAAGCTGCTTATATTTTGAGTACAGGTTTCACTATTAC
  AAATATATGATGTTAAACTAACAAACTCATGACCTTCAAAGATGTCTTCGTCCCACGCAC
  ACACATTTGTAATTTGTGTCCATTTGCTATTTCCCTTCTTCTATAATCTTCAAATTATAT
  AGTTATGCATTGAGTTCCCTATGCATCTCACCCATCTCCTTTATCTCAGCCTTCTCATAC
  TTTGCCATTCTCTTCTTTCTGGAAATAACCAGCACAACATTTCCAGCAACAACTGCTATC
  ACCACAACCACATTAACAGCATTAACACCAGCTTTTAGACCCTGCATTGAGAATTCAGGT
  GCTTTTTCATCAACATAATAAATTAAAGTTTGACCAGGATCCAGATCCAGTTGTTCCCCA
  TTTACTGTCAGGTCCATTTTCTTAGAATGAAACAAGGATTCACCTTTAACATCTTTTTCA
  AAATAATAAGCCACATCAGCTATGTCCACATCATTCTGAGTTTTTTGAGAAGATTTTTGA
  ACCAGATCAATAGTGATAACATTATTCTCATACAAATACTCGAGATAAATTTTGGATACA
  GTTGATAACGCGTTGTGATCCTCCTCCTGAGTGCACGCCCGCAACTTTTACTATCATAAA
  GGTTTTCTCTTGCTTGAGTTTTAGTTAAAGATGATCAGTAGTTCCAACTCGCTCAGAGCA
  GTTATTTAATGTCCTTGTCTGTTCTTTGACCCCACAGGGTTCACACCCAACCAGGAAGGT
  GCCGTTCACTGCTTGGCCTTAATGACCCGCTTCATCGCATCCAGGATCTTATGCCCTCCT
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002354
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002354.1, NP_002345.1
• RefSeq Size: 1528 bp
• RefSeq ORF: 945 bp
• Locus ID: 4072
• UniProt ID: P16422
• Cytogenetics: 2p21
• Domains: thyroglobulin_1
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]