SH2D1A (NM_002351) Human Untagged Clone

SKU SC118690

SH2D1A (untagged)-Human SH2 domain containing 1A (SH2D1A), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SH2D1A
• Synonyms: DSHP; EBVS; IMD5; LYP; MTCP1; SAP; SAP/SH2D1A; XLP; XLPD; XLPD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002351 edited; ATGGACGCAGTGGCTGTGTATCATGGCAAAATCAGCAGGGAAACCGGCGAGAAGCTCCTG; CTTGCCACTGGGCTGGATGGCAGCTATTTGCTGAGGGACAGCGAGAGCGTGCCAGGCGTG; TACTGCCTATGTGTGCTGTATCACGGTTACATTTATACATACCGAGTGTCCCAGACAGAA; ACAGGTTCTTGGAGTGCTGAGACAGCACCTGGGGTACATAAAAGATATTTCCGGAAAATA; AAAAATCTCATTTCAGCATTTCAGAAGCCAGATCAAGGCATTGTAATACCTCTGCAGTAT; CCAGTTGAGAAGAAGTCCTCAGCTAGAAGTACACAAGGTACTACAGGGATAAGAGAAGAT; CCTGATGTCTGCCTGAAAGCCCCATGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002351 unedited; GGTCAAAATTTGTAAACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGGAGTCAGG; TGGTTGACTTGTGCCTGGCTGCAGTAGCAGCGGCATCTCCCTTGCACAGTTCTCCTCCTC; GGCCTGCCCAAGAGTCCACCAGGCCATGGACGCAGTGGCTGTGTATCATGGCAAAATCAG; CAGGGAAACCGGCGAGAAGCTCCTGCTTGCCACTGGGCTGGATGGCAGCTATTTGCTGAG; GGACAGCGAGAGCGTGCCAGGCGTGTACTGCCTATGTGTGCTGTATCACGGTTACATTTA; TACATACCGAGTGTCCCAGACAGAAACAGGTTCTTGGAGTGCTGAGACAGCACCTGGGGT; ACATAAAAGATATTTCCGGAAAATAAAAAATCTCATTTCAGCATTTCAGAAGCCAGATCA; AGGCATTGTAATACCTCTGCAGTATCCAGTTGAGAAGAAGTCCTCAGCTAGAAGTACACA; AGGTACTACAGGGATAAGAGAAGATCCTGATGTCTGCCTGAAAGCCCCATGAAGAANAAT; AAAACACCTTGTACTTTATTTTCTATAATTTAAATATATGCTAAGTCTTATATATTGTAG; ATAATACAGTTCGGTGAGCTACAAATGCATTTCTAAAGCCATTGTAGTCCTGTAATGGAA; GCATCTAGCATGTCGTCAAAGCTGANATGGACTTTTGTACATAGTGAGGAGCTTNTGAAC; GAGGATTGGGAAAAAGNTATTCCCCGTAGTATTTTCAGTTATTATATTTACAAATGGGAA; ACAAANGGATNATGAATACTTTATAAAGGATTAAATGTCATTTCTGNNCCAATATAATAA; AAATATCCTCCATTTTTTGTGAAAAGCTTCATTTTTAGTGAAATATATTTTATAGCTACT; AATTTTAAAANTGTCTGCNNTGATGATGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002351 unedited; NTTCTACTTGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAGCTT; TCAAACACTTTATTTAAATGTGAAAGACTTGATGATTCAAAAAAAGCTTTTTTTCAAAAA; AAGGCTCTTGGAAGTGAACATGCAAACATAATTCATATGTTTAGGATTATTCCAGAATGA; ACACATTATAGAGTTAAAATTAGTTACGCCTATTACAATTTAAACTCAAGCCATATAATT; AAAAATTATTAAACCTATGCAACTTATTATGAAAGTAGGATTTTTAAATTCCTTTACAAG; TTCATGTGATGCTTTTACCTCAAGAAATGGTAAACCACCCATAATGCATTACTCAGGCCA; CACTCCACTTTTGTACTCCCACGGCATCCATATTAAATGCTCAGAAATCAGGTCTTGCCA; GTACATGGAGAATAGCTCTGTCTCTCAGCAGTTCACTAATTAAAACCAAGTTCACCAACA; CCCATACACAATCCAGAATTCCCAGTTATTATTTTACAGTGAAGCTTAGCCTTTTCATAT; GGCCCATGGACCAGAGTATTTGCACTGACCTATCTATCTGCCTCTAAAGACCAGATCTAC; CTACACACGGGGAACCTCGCTTATCATGTACTTATCAACTTTCATTGGTATTTTTTCCTT; GGCTTTTATTGGTATTNTCTTTCACTGGCTTTTATCGNTATTTTCTTTCACTGTCTTTTA; TCGNTATTGTTTTCTCGTCCACAAGACAGTGAACTGAGAGCAGGGACCAGGTGTTTTTAC; TTCTATGCCCTCAGCGCCCTATTATGTNTGNTGAGTGAATGAANTCTGTGAGCCCTGTNG; CAAAGTGACATANTATCAATCACAAAATCACCAAAATATTAATGTGAGAAGAAAATGCCG; CATAAAATCTGTATCTCGTGGTAGTTTCAATCACTTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002351
• Insert Size: 2390 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002351.1, NP_002342.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 387 bp
• Locus ID: 4068
• UniProt ID: O60880
• Cytogenetics: Xq25
• Domains: SH2
• Protein Families: Druggable Genome
• Protein Pathways: Natural killer cell mediated cytotoxicity
• Summary: This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).