MDM2 (NM_002392) Human Untagged Clone

CAT#: SC118660

MDM2 (untagged)-Human Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MDM2
• Synonyms: ACTFS; hdm2; HDMX; LSKB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002392 edited
  AGGGCGTCGTGCTTCCGCGCGCCCCGTGAAGGAAACTGGGGAGTCTTGAGGGACCCCCGA
  CTCCAAGCGCGAAAACCCCGGATGGTGAGGAGCAGGCAAATGTGCAATACCAACATGTCT
  GTACCTACTGATGGTGCTGTAACCACCTCACAGATTCCAGCTTCGGAACAAGAGACCCTG
  GTTAGACCAAAGCCATTGCTTTTGAAGTTATTAAAGTCTGTTGGTGCACAAAAAGACACT
  TATACTATGAAAGAGGTTCTTTTTTATCTTGGCCAGTATATTATGACTAAACGATTATAT
  GATGAGAAGCAACAACATATTGTATATTGTTCAAATGATCTTCTAGGAGATTTGTTTGGC
  GTGCCAAGCTTCTCTGTGAAAGAGCACAGGAAAATATATACCATGATCTACAGGAACTTG
  GTAGTAGTCAATCAGCAGGAATCATCGGACTCAGGTACATCTGTGAGTGAGAACAGGTGT
  CACCTTGAAGGTGGGAGTGATCAAAAGGACCTTGTACAAGAGCTTCAGGAAGAGAAACCT
  TCATCTTCACATTTGGTTTCTAGACCATCTACCTCATCTAGAAGGAGAGCAATTAGTGAG
  ACAGAAGAAAATTCAGATGAATTATCTGGTGAACGACAAAGAAAACGCCACAAATCTGAT
  AGTATTTCCCTTTCCTTTGATGAAAGCCTGGCTCTGTGTGTAATAAGGGAGATATGTTGT
  GAAAGAAGCAGTAGCAGTGAATCTACAGGGACGCCATCGAATCCGGATCTTGATGCTGGT
  GTAAGTGAACATTCAGGTGATTGGTTGGATCAGGATTCAGTTTCAGATCAGTTTAGTGTA
  GAATTTGAAGTTGAATCTCTCGACTCAGAAGATTATAGCCTTAGTGAAGAAGGACAAGAA
  CTCTCAGATGAAGATGATGAGGTATATCAAGTTACTGTGTATCAGGCAGGGGAGAGTGAT
  ACAGATTCATTTGAAGAAGATCCTGAAATTTCCTTAGCTGACTATTGGAAATGCACTTCA
  TGCAATGAAATGAATCCCCCCCTTCCATCACATTGCAACAGATGTTGGGCCCTTCGTGAG
  AATTGGCTTCCTGAAGATAAAGGGAAAGATAAAGGGGAAATCTCTGAGAAAGCCAAACTG
  GAAAACTCAACACAAGCTGAAGAGGGCTTTGATGTTCCTGATTGTAAAAAAACTATAGTG
  AATGATTCCAGAGAGTCATGTGTTGAGGAAAATGATGATAAAATTACACAAGCTTCACAA
  TCACAAGAAAGTGAAGACTATTCTCAGCCATCAACTTCTAGTAGCATTATTTATAGCAGC
  CAAGAAGATGTGAAAGAGTTTGAAAGGGAAGAAACCCAAGACAAAGAAGAGAGTGTGGAA
  TCTAGTTTGCCCCTTAATGCCATTGAACCTTGTGTGATTTGTCAAGGTCGACCTAAAAAT
  GGTTGCATTGTCCATGGCAAAACAGGACATCTTATGGCCTGCTTTACATGTGCAAAGAAG
  CTAAAGAAAAGGAATAAGCCCTGCCCAGTATGTAGACAACCAATTCAAATGATTGTGCTA
  ACTTATTTCCCCTAGTTGACCTGTCTATAAGAGAATTATATATTTCTAACTATATAACCC
  TAGGAATTTAGACCACCTGAAATTTATTCACATATATCAAAGTGAGAAAATGCCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002392 unedited
  GCAACGCAATTCGCACGAGCCTCCCCGGATTAGTGCGTACGAGCGCCCAGTGCCCTGGCC
  CGGAGAGTGGAATGATCCCCGAGGCCCAGGGCGTCGTGCTTCCGCGCGCCCCGTGAAGGA
  AACTGGGGAGTCTTGAGGGACCCCCGACTCCAAGCGCGAAAACCCCGGATGGTGAGGATC
  AGGTAAATGTGCAATACCAACATGTCTGTACCTACTGATGGTGCTGTAACCACCTCACAG
  ATTCCAGCTTCGGAACAAGAGACCCTGGTTAGACCAAAGCCATTGCTTTTGAAGTTATTA
  AAGTCTGTTGGTGCACAAAAAGACACTTATACTATGAAAGAGGTTCTTTTTTATCTTGGC
  CAGTATATTATGACTAAACGATTATATGATGAGAAGCAACAACATATTGTATATTGTTCA
  AATGATCTTCTAGGAGATTTGTTTGGCGTGCCAAGCTTCTCTGTGAAAGAGCACAGGAAA
  ATATATACCATGATCTACAGGAACTTGGTAGTAGTCAATCAGCAGGAATCATCGGACTCA
  GGTACATCTGTGAGTGAGAACAGGTGTCACCTTGAAAGTGGGAGTGATCAAAAGGACCTT
  GTACAAGAGCTTCANGAAGAGAAACCTTCATCTTCACATTTGGTTTCTAGACCATCTACC
  TCATCTAGAAAGGAGAGCCATTAGTGAGACAGAAGAAAATTCATATGAATTATCTGGTGA
  ACGACCAAANAAAGCGCCCCAAACCTGATAGTATTTCCCTTTCCTTTTGTGAAAAGCCTG
  CCTCTGTGTGGTAAAAAGGGAGATATGTTGGGAAAAGAAGCATTANCCGTGGAATCTACA
  GGGCCCCCATTCGAATCCGGATCTTGATGCCTGGGGTAAAGGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002392 unedited
  AAAANTTTGACTCTGGACCCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTAAAT
  AAGTTACATTTAAATGTCATATACACTATTTAAGAAAAAAAAAAACCAGTACTTCTCATT
  TAAGACAGAGTAGAAATTATTTAAAATTAGGAGTTGGTGTAAAGGATGAGCTACATATTC
  AAGTCAAATTATAGTAAGTATTCACTATTCCACTACCAAAGTAGGTCAATTATACTAAAG
  AGAAGAAATCTATGTGAATTGAGGCATTTTCTCACTTTGATATATGTGAATAAATTTCAG
  GTTGTCTAAATTCCTAGGGTTATATAGTTAGAAATATATAATTCTCTTATAGACAGGTCA
  ACTAGGGGAAATAAGTTAGCACAATCATTTGAATTGGTTGTCTACATACTGGGCAGGGCT
  TATTCCTTTTCTTTAGCTTCTTTGCACATGTAAAGCAGGCCATAAGATGTCCTGTTTTGC
  CATGGACAATGCAACCATTTTTAGGTCGACCTTGACAAATCACACAAGGTTCAATGGCAT
  TAAGGGGCAAACTAGATTCCACACTCTCTTCTTTGTCTTGGGTTTCTTCCCTTTCAAACT
  CTTTCACATCTTCTTGGCTGCTATAAATAATGCTACTAGAAGTTGATGGCTGAGAATAGT
  CTTCACTTTCTTGTGATTGTGAAGCTGNGTAATTTTATCATCATTTTCCTCACACATGAC
  TCTCTGGATCATCACTATAGGTTTTTTACATCACGAACTCAAAGCCTCTTCAGCTTGGGT
  GAGTTTCCAGTTGGCTTTTTAGAAATTCCCCTTATCTTTCCCTTTATCTCAGGAGCCCAT
  TTCACCAAGGCCCAACATTTTGCATTTGTGGAAAGGGGGGAATCTTCATTGCTGAAAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002392
• Insert Size: 1800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002392.2, NP_002383.2
• RefSeq Size: 2357 bp
• RefSeq ORF: 1494 bp
• Locus ID: 4193
• UniProt ID: Q00987
• Cytogenetics: 12q15
• Domains: zf-RanBP, MDM2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Endocytosis, Glioma, Melanoma, p53 signaling pathway, Pathways in cancer, Prostate cancer, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.