c-Myc (MYC) (NM_002467) Human Untagged Clone

CAT#: SC112715

MYC (untagged)-Human v-myc myelocytomatosis viral oncogene homolog (avian) (MYC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYC
• Synonyms: bHLHe39; c-Myc; MRTL; MYCC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002467 edited
  ATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG
  CCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTG
  CAGCCCCCGGCGCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCC
  CTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTC
  TCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCAGCTGGAG
  ATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGAC
  GACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGGCTTCTCGGCC
  GCCGCCAAGCTCGTCTCAGAGAAGCTGGCCTCCTACCAGGCTGCGCGCAAAGACAGCGGC
  AGCCCGAACCCCGCCCGCGGCCACAGCGTCTGCTCCACCTCCAGCTTGTACCTGCAGGAT
  CTGAGCGCCGCCGCCTCAGAGTGCATCGACCCCTCGGTGGTCTTCCCCTACCCTCTCAAC
  GACAGCAGCTCGCCCAAGTCCTGCGCCTCGCAAGACTCCAGCGCCTTCTCTCCGTCCTCG
  GATTCTCTGCTCTCCTCGACGGAGTCCTCCCCGCAGGGCAGCCCCGAGCCCCTGGTGCTC
  CATGAGGAGACACCGCCCACCACCAGCAGCGACTCTGAGGAGGAACAAGAAGATGAGGAA
  GAAATCGATGTTGTTTCTGTGGAAAAGAGGCAGGCTCCTGGCAAAAGGTCAGAGTCTGGA
  TCACCTTCTGCTGGAGGCCACAGCAAACCTCCTCACAGCCCACTGGTCCTCAAGAGGTGC
  CACGTCTCCACACATCAGCACAACTACGCAGCGCCTCCCTCCACTCGGAAGGACTATCCT
  GCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGATCAGCAACAACCGA
  AAATGCACCAGCCCCAGGTCCTCGGACACCGAGGAGAATGTCAAGAGGCGAACACACAAC
  GTCTTGGAGCGCCAGAGGAGGAACGAGCTAAAACGGAGCTTTTTTGCCCTGCGTGACCAG
  ATCCCGGAGTTGGAAAACAATGAAAAGGCCCCCAAGGTAGTTATCCTTAAAAAAGCCACA
  GCATACATCCTGTCCGTCCAAGCAGAGGAGCAAAAGCTCATTTCTGAAGAGGACTTGTTG
  CGGAAACGACGAGAACAGTTGAAACACAAACTTGAACAGCTACGGAACTCTTGTGCGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002467 unedited
  TAGATTTCTGTACACGATTCACTATAGGGCGGCCGCGCAATTCGCACGAGGCGGCTAGGG
  TGGAAGAGCCGGGCGAGCAGAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCG
  AATAGGGGGCTTCGCCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAA
  CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACT
  TACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGG
  GGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTT
  AGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGCCTCCCGCGACGATGCCCCTCAACG
  TTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACT
  GCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCCAGAGCGAGCTGCAGCCCCCGGCG
  CCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCCCGCCCCTGTCCCCTAGC
  CGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGA
  GACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCAGCTGTAGATGGTGACCGAG
  CTGCTGCGAGGAGACATGCTGAACCACAGTTTCATCTGCGACCCGGACGACGAGACCTTC
  ATCAAAAACATCATCATCCAGGACTGTATGTGGGAGCGGCTTTTTCGGCGCCGCCAAGCT
  CGTCTCAGAGAGCTGGGCCTCTACAGGGCTGAGCGCAAGACAGAGGNAGACCGAACCCGC
  CCGCGGGCCCAGNGTCTGCTCCACCTCCACTTGTACTGCAGTATCTGACGCCGCGCTCAC
  ATGCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002467 unedited
  TACTTTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATATTTGC
  CAGTTATTTTATTTTTTCTAAAAACAATAGAAAAAATCAACTTTAAAAAGCAAAATGTAC
  TTAAATAAAAAAAATTAGGGTTTATAGTACCTATAATACTAGGTACTATATACTAGGATT
  GAAATTCTGTGTAACTGCTATAAACGTTTTNATTAAAGTTNTATTTACATTTAATGGCAA
  TATTTACAGAGAAACATTGTGTAAATCTTAAAATTTTTTAAAAACAATTCTTAAATACAA
  ATCTGTTAAAGAAAAAAAAAAGATGGTAAGCATAAAAAAGTTCTTTTATGCCCAAAGTCC
  AATTTGAGGCAGTTTACATTATGGCTAAATCTTTCAGTCTCAAGACTCAGCCAAGGTTGT
  GAGGTTGCATTTGATCATGCATTTGAAACAAGTTCATAGGTGATTGCTCANGACATTTCT
  GTTAGAAGGAAATCGTTTCCTTACTTTTCCTTACGCACAAAGAGTCCGTAGCTGTTCAAG
  TTTGTGTTTCAACTGTTCTCGTCGTTTCCGCAACAAGTCCTCTTCAGAAATGAGCTTTTG
  CTCCTCTGCTTGNACGGACAGGATGTATGCTGTGGNCTTTTTAANGGATAACTACCTTNG
  GGGGNCCCTTTCATGNTTTCCAACTNCCGGATCTGGTCACGCAGGGCAAAAAAGCTNCGT
  TTTAACTCGTTTCTTCTCTGGCGCTNCAAGACGTTGGGTGTTCGCCTCTTGACATTCTCC
  TCGGTGTCCGAAGACCTGGGGNCTGTGCATTTTCCGGTGGTGCTGATCTGCTTAAGACTT
  TGACACTGTCAACTTGACCCTTTTGCAACAGAAAGTCCTTCGAATGAAGGGAGCCCTGCC
  TAATTGCCTAATGTTGGAAACTGGGCACTCTTGAGAACAAGGGCCTTAAGAGGTTTTCTG
  GGCCTCACCAAAAGGAACCAACTTTGCCCTTTGCCAAAC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002467
• Insert Size: 2100 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002467.3, NP_002458.2
• RefSeq Size: 2377 bp
• RefSeq ORF: 1365 bp
• Locus ID: 4609
• UniProt ID: P01106
• Cytogenetics: 8q24.21
• Domains: HLH, Myc_N_term, Myc-LZ
• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway
• Gene Summary: This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]