Iba1 (AIF1) (NM_032955) Human Untagged Clone

CAT#: SC108920

AIF1 (untagged)-Human allograft inflammatory factor 1 (AIF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Iba1
• Synonyms: AIF-1; IBA1; IRT-1; IRT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108920 sequence for NM_032955 edited (data generated by NextGen Sequencing)
  ATGGAGTTTGACCTTAATGGAAATGGCGATATTGATATCATGTCCCTGAAACGAATGCTG
  GAGAAACTTGGAGTCCCCAAGACTCACCTAGAGCTAAAGAAATTAATTGGAGAGGTGTCC
  AGTGGCTCCGGGGAGACGTTCAGCTACCCTGACTTTCTCAGGATGATGCTGGGCAAGAGA
  TCTGCCATCCTAAAAATGATCCTGATGTATGAGGAAAAAGCGAGAGAAAAGGAAAAGCCA
  ACAGGCCCCCCAGCCAAGAAAGCTATCTCTGAGTTGCCCTGA
  
  Clone variation with respect to NM_032955.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032955 unedited
  NGGGTGCACATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCA
  ACCCCTTCCTCAGTCCCCGGCTGAAAACCCTCGAGTCAGCGCTTATCCCTTCTGCTCTCT
  CCCCTCACCCAGAGAAATACATGGAGTTTGACCTTAATGGAAATGGCGATATTGATATCA
  TGTCCCTGAAACGAATGCTGGAGAAACTTGGAGTCCCCAAGACTCACCTAGAGCTAAAGA
  AATTAATTGGAGAGGTGTCCAGTGGCTCCGGGGAGACGTTCAGCTACCCTGACTTTCTCA
  GGATGATGCTGGGCAAGAGATCTGCCATCCTAAAAATGATCCTGATGTATGAGGAAAAAG
  CGAGAGAAAAGGAAAAGCCAACAGGCCCCCCAGCCAAGAAAGCTATCTCTGAGTTGCCCT
  GATTTGAAGGGAAAAGGGATGATGGGATTGAAGGGGCTTCTAATGACCCAGATATGGAAA
  CAGAAGACAAAATTGTAAGCCAGAGTCAACAAATTAAATAAATTACCCCCTCCTCCAGAT
  CANANAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAGACTAGTTCTCCTCTAGATTG
  CGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCC
  CAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAA
  AATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAGGGG
  GGGTGGTATGGAGCAAGGNGCAAGTNGGGAAGNACACCTGTANGGCCTGNCGGGTCTATT
  GGGAACCAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGNCATCTCCGCTCCTGGGGT
  CAGCGATTTNCTGCCTCAGCTNCCCGAGTGTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032955 unedited
  NGTCCTAGCTTGCCCGCGCCCGCAATCTANAGNANAACTAGTCTCGGTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTGATCCGGAGGAGGGGGTAATTTATTTAATTTGTTGACTCT
  GGCTTACAATTTTGTCTTCTGTTTCCATATCTGGGTCATTAAAAGCCCCTTCAATCCCAT
  CATCCCTTTTCCCTTCAAATCAGGGCAACTCAAAAATAGCTTTCTTGGCTGGGGGGCCTG
  GTGGCTTTTCCTTTTCTCTCGCTTTTTCCTCATACATCAGGATCATTTTTAGGATGGCAG
  ATCTCTTGCCCAGCATCATCCTGAGAAAGTCAGGGTAGCTGAACGTCTCCCCGGAGCCAC
  TGGACACCTCTCCAATTAATTTCTTTAGCTCTAGGTGAGTCTTGGGGACTCCAAGTTTCT
  CCAGCATTCGTTTCAGGGACATGATATCAATATCGCCATTTCCATTAAGGGCAAACTCCA
  TGTATTTCTCTGGGTGAGGGGAGAGAGCAAAAGGGATAAGCGCTGACTCGAGGGTTTTCA
  GCCGGGGACTGAGGAAGGGGTTGCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTC
  GTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTA
  CACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGCTATTACGACATTTTGGAAAGCCCC
  GTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTCGGAAATC
  CCCGTGAGTCAAACCGCTATCCAGCCCATTGTTGTACTGGCAAACCGCATACCCTGTAAT
  ACCGATGACTATACGTAAATGTACTGCCAGTACGAAAGTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032955
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032955.1, NP_116573.1
• RefSeq Size: 503 bp
• RefSeq ORF: 282 bp
• Locus ID: 199
• UniProt ID: P55008
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1, also known as G1) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 3. The encoded isoform (1) has a shorter N-terminus than isoform 3. Variants 1 and 4 encode the same isoform (1).