Iba1 (AIF1) (NM_032955) Human Untagged Clone

SKU SC108920

AIF1 (untagged)-Human allograft inflammatory factor 1 (AIF1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Iba1
• Synonyms: AIF-1; IBA1; IRT-1; IRT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108920 sequence for NM_032955 edited (data generated by NextGen Sequencing); ATGGAGTTTGACCTTAATGGAAATGGCGATATTGATATCATGTCCCTGAAACGAATGCTG; GAGAAACTTGGAGTCCCCAAGACTCACCTAGAGCTAAAGAAATTAATTGGAGAGGTGTCC; AGTGGCTCCGGGGAGACGTTCAGCTACCCTGACTTTCTCAGGATGATGCTGGGCAAGAGA; TCTGCCATCCTAAAAATGATCCTGATGTATGAGGAAAAAGCGAGAGAAAAGGAAAAGCCA; ACAGGCCCCCCAGCCAAGAAAGCTATCTCTGAGTTGCCCTGA; ; Clone variation with respect to NM_032955.1;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032955 unedited; NGGGTGCACATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCA; ACCCCTTCCTCAGTCCCCGGCTGAAAACCCTCGAGTCAGCGCTTATCCCTTCTGCTCTCT; CCCCTCACCCAGAGAAATACATGGAGTTTGACCTTAATGGAAATGGCGATATTGATATCA; TGTCCCTGAAACGAATGCTGGAGAAACTTGGAGTCCCCAAGACTCACCTAGAGCTAAAGA; AATTAATTGGAGAGGTGTCCAGTGGCTCCGGGGAGACGTTCAGCTACCCTGACTTTCTCA; GGATGATGCTGGGCAAGAGATCTGCCATCCTAAAAATGATCCTGATGTATGAGGAAAAAG; CGAGAGAAAAGGAAAAGCCAACAGGCCCCCCAGCCAAGAAAGCTATCTCTGAGTTGCCCT; GATTTGAAGGGAAAAGGGATGATGGGATTGAAGGGGCTTCTAATGACCCAGATATGGAAA; CAGAAGACAAAATTGTAAGCCAGAGTCAACAAATTAAATAAATTACCCCCTCCTCCAGAT; CANANAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAGACTAGTTCTCCTCTAGATTG; CGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCC; CAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAA; AATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAGGGG; GGGTGGTATGGAGCAAGGNGCAAGTNGGGAAGNACACCTGTANGGCCTGNCGGGTCTATT; GGGAACCAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGNCATCTCCGCTCCTGGGGT; CAGCGATTTNCTGCCTCAGCTNCCCGAGTGTTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032955 unedited; NGTCCTAGCTTGCCCGCGCCCGCAATCTANAGNANAACTAGTCTCGGTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTGATCCGGAGGAGGGGGTAATTTATTTAATTTGTTGACTCT; GGCTTACAATTTTGTCTTCTGTTTCCATATCTGGGTCATTAAAAGCCCCTTCAATCCCAT; CATCCCTTTTCCCTTCAAATCAGGGCAACTCAAAAATAGCTTTCTTGGCTGGGGGGCCTG; GTGGCTTTTCCTTTTCTCTCGCTTTTTCCTCATACATCAGGATCATTTTTAGGATGGCAG; ATCTCTTGCCCAGCATCATCCTGAGAAAGTCAGGGTAGCTGAACGTCTCCCCGGAGCCAC; TGGACACCTCTCCAATTAATTTCTTTAGCTCTAGGTGAGTCTTGGGGACTCCAAGTTTCT; CCAGCATTCGTTTCAGGGACATGATATCAATATCGCCATTTCCATTAAGGGCAAACTCCA; TGTATTTCTCTGGGTGAGGGGAGAGAGCAAAAGGGATAAGCGCTGACTCGAGGGTTTTCA; GCCGGGGACTGAGGAAGGGGTTGCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTC; GTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTA; CACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGCTATTACGACATTTTGGAAAGCCCC; GTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTCGGAAATC; CCCGTGAGTCAAACCGCTATCCAGCCCATTGTTGTACTGGCAAACCGCATACCCTGTAAT; ACCGATGACTATACGTAAATGTACTGCCAGTACGAAAGTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_032955
• Insert Size: 600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032955.1, NP_116573.1
• RefSeq Size: 503 bp
• RefSeq ORF: 282 bp
• Locus ID: 199
• UniProt ID: P55008
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Summary: This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1, also known as G1) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 3. The encoded isoform (1) has a shorter N-terminus than isoform 3. Variants 1 and 4 encode the same isoform (1).