Cytochrome b5 Outer Mitochondrial Membrane (CYB5B) (NM_030579) Human Untagged Clone

SKU
SC107995
CYB5B (untagged)-Human cytochrome b5 type B (outer mitochondrial membrane) (CYB5B), nuclear gene encoding mitochondrial protein
$150.00
In Stock*
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Cytochrome b5 Outer Mitochondrial Membrane
Synonyms CYB5-M; CYPB5M; OMB5
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_030579, the custom clone sequence may differ by one or more nucleotides


ATGTCCGGTTCAATGGCGACTGCGGAAGCTAGCGGCAGCGATGGGAAAGGGCAGGAAGTCGAGACCTCAG
TCACCTATTACCGGTTGGAGGAGGTGGCAAAGCGCAACTCCTTGAAGGAACTGTGGCTTGTGATCCATGG
GCGAGTCTACGATGTCACCCGCTTCCTCAACGAGCACCCTGGAGGAGAAGAGGTTCTGCTGGAACAAGCT
GGTGTAGATGCAAGTGAAAGCTTTGAAGATGTAGGACACTCTTCTGATGCCAGAGAAATGCTAAAGCAGT
ACTACATTGGTGATATCCATCCGAGTGACCTTAAACCTGAAAGTGGTAGCAAGGACCCTTCAAAAAATGA
TACATGCAAAAGTTGCTGGGCATATTGGATTTTACCCATCATAGGCGCTGTTCTCTTAGGTTTCCTGTAC
CGCTACTACACATCGGAAAGCAAATCCTCCTGA


5' Read Nucleotide Sequence
>OriGene 5' read for NM_030579 unedited
CACTATATTTGTATCACGACTCACTATAGGGCGGCCGCGCAATTCGCACGAGGGTCCGGT
TCAATGGCGACTGCGGAAGCTAGCGGCAGCGATGGGAAAGGGCAGGAAGTCGAGACCTCA
GTCACCTATTACCGGTTGGAGGAGGTGGCAAAGCGCAACTCCTTGAAGGAACTGTGGCTT
GTGATCCATGGGCGAGTCTACGATGTCACCCGCTTCCTCAACGAGCACCCTGGAGGAGAA
GAGGTTCTGCTGGAACAAGCTGGTGTAGATGCAAGTGAAAGCTTTGAAGATGTAGGACAC
TCTTCTGATGCCAGAGAAATGCTAAAGCAGTACTACATTGTGTGATATCCATCCGAGTGA
CCTTAAACCTGAAAGTGGTAGCAAGGACCCTTCAAAAAATGATACATGCAAAAGTTGCTG
GGCATATTGGATTTTACCCATCATAGGCGCTGTTCTCTTAGGTTTCCTGTACCGCTACTA
CACATCGGAAAGCAAATCCTCCTGAGGAGGCCTTGCTGAAGTTAGAAAGTGCATCCACTT
TGGGGCGAAAACTAGAGACTTGCTTGGGGGCTGCAGAAGTGCCCTCTCCTTGAATCCTGC
CAGTTGCATTCTTTCCCCTTGGACCAAGACCATTGGCCAGACATTACCTCAGATCTGAGA
CCAACGTTCTCCATCTTTCAGAGCCTTACTCCCAAAGTACCTGCTCACTGTTCCGTGTTG
AACCATTGCCGGCGTTTCTTCTTTTCACTGGGTTCCTGCGCACCTTCAATTTTACAACCT
TTTGTTCTTAAATTTATGGACATTGCTTTTTGGAAAAAAGCTGCTTTCCCACACTTTGTT
GCATATAAACATCCTTAACAGGCGGCATCTTATGTTAAAGGTTATTTTTCCTTTTTTTTT
AAGGAAATTTTTCAAAACTTCGGTTACGGTT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_030579 unedited
GACAGGCACGCTTTATTCTAGTGTACGAGCTTTTCTTTCTTTTTCTTTTCTCTCGAAAAA
AACACGCCAGCTATCTTTATCTCTCATGGGAAAAACAGAACAAACCCACAAGTTGGAGTC
ACGGAGATAAAATACAGATGAAATGGAAAACGGTCTGTTGTCATGAACTCTCACTTTCAA
ATACCATTTTATATGGAAGTTACTTTACTGCGGGGCAAACAGAAGGCCATGCTGGAGTCT
CTTACTTTTGGAAAATGGAGAATCAAAAATTTGCTAATCAACAAACAAAAAAGGAGGGAA
ACTCCTTGGTAAAGCTCTACAAACATAATTATACATTTATATTTTACCAATAAAAGATAG
CTAGGGTAGAAAAAACAGATGGTTAGAAACTGGTGCCAAACCAAAGTGAAAGCTTTGGTG
CCTTCTCTAAACTCCTATCCTGTTTCTTTAGAAAACACCAGTTTTCTAAAGAAAGATTAC
TCTGAATTCACCAGGGTTCTATCACCCCAATTCATCCCTACCTTTCCACCCCCAAGACAC
GAAAGGGCCATGTAGTTTTTTGTCCGGCACCACTTGGGAAGGGGCTGGGCTCAAGGGCTG
GGAAGAGCTACCTGCTGCCCTAGCTTGAAAGGGAGGAGGTGGCCACACCATTCTTTGTAT
TTTTATCACGCACATAAAAGAACCAAAACCATTTGGCAGCTGTAAGTTTAATAAGCGCAA
ACCTTTACTTTAGGAATTAAACCCCCTATGATAAAAAAAGAAATAAATCACATTTTTTTT
TTATACAGATAGGGGTCTCGCTTGCTGCCCAGCCTGGTCTGAACCCTTGCCCTTAGCGAT
CTTTCCACCTTGGCTCCAAATGGCAGGATTACAGCCCGAGCCACCACCTACCCTTATCAA
AATTTTTAAAAATATTACTCAAAAAAGAGGCAAATAAACCTTCAACCTCACTGCCCCCTC
TTAAAATGCAAATGCATCAAAC
Restriction Sites NotI-NotI
ACCN NM_030579
Insert Size 1820 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030579.1, NP_085056.1
RefSeq Size 4286 bp
RefSeq ORF 441 bp
Locus ID 80777
UniProt ID O43169
Cytogenetics 16q22.1
Domains heme_1
Protein Families Transmembrane
Summary Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.