Setsip (NM_194353) Rat Tagged ORF Clone

SKU
RR213325
Setl1 (Myc-DDK-tagged ORF) - Rat SET translocation-like 1 (Setl1), (10 ug)
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    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$330.00
4 Weeks*
Specifications
Product Data
Type Rat Tagged ORF Clone
Target Symbol Setsip
Synonyms RGD727855; Set; Setl1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RR213325 representing NM_194353
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCGAAGCGGCAATCTGCGATCCCGCCTCAGCCCAAGAAACCCAGACCCATTGCTGCCCCGAAGT
TGGAGGACAAGTTGGCCTCTCCCAGCCTGCCGAAGGGAGAAAAAGAACAGCAAGAAACTATTGACCATAT
TGATGAAGTACAAAATGAAATGGACAGACTTAATGAACAACCCAGTGAGGAAATTTTGAAAGTAGAACAA
AAATATAACAGACTCTGCCAACCATTTTTTTTTCAGAAGAGGTCAGAATTGATCGCCAAAATCCCAAATT
TTTGGGTAACAACATTTGTCAACCATCCACAAGTGTCTGCACCGCGTGGGGAGGAAGACGAGGAGGCCCT
GCATTATTTGACCAGAGTTGAAGGGACAGAATTTGGAGACATTAAATCAGGTTACAGAATAGATTTTTAT
TTTGATGAGAATCCTTACTTTGAAAATAAAGTTCTCTCCAAAGAATTTCATCTGAATGAGAGCGGTGACC
CGTCTTCAAAGTCCACCGAAATCAAATGGAAATCTGGGAAGGATTTGACAAAATGCTCAAGTCAAACGCC
GAATAAGGCCAGCAGGAAGAGGCAGCGTGAAAAGCCAGAGAGTTCCTTTCCCTGGTTTACTGACCGTTCT
GACGCAGGTGCTGACGAGTTAGGAGAGGTCGTCAAGGATAATATTTGGCCAAACCCCTTGCGGTCCTATT
TGGTTCCCGATATGGGTGATGAAGGAGAGGCAGAGGATGATGATGATGACGACGACGAAGAAGAACCGTT
GGAAGATATTGATGAAGGAGACAGGGATGAAGGTGAAGAAGATGACGATGAGGAGGAAGGGGAGGAAGGA
GGACGAAGGCGAGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RR213325 representing NM_194353
Red=Cloning site Green=Tags(s)

MAPKRQSAIPPQPKKPRPIAAPKLEDKLASPSLPKGEKEQQETIDHIDEVQNEMDRLNEQPSEEILKVEQ
KYNRLCQPFFFQKRSELIAKIPNFWVTTFVNHPQVSAPRGEEDEEALHYLTRVEGTEFGDIKSGYRIDFY
FDENPYFENKVLSKEFHLNESGDPSSKSTEIKWKSGKDLTKCSSQTPNKASRKRQREKPESSFPWFTDRS
DAGADELGEVVKDNIWPNPLRSYLVPDMGDEGEAEDDDDDDDEEEPLEDIDEGDRDEGEEDDDEEEGEEG
GRRRG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_194353
ORF Size 855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194353.1, NP_919334.1
RefSeq Size 858 bp
RefSeq ORF 858 bp
Locus ID 290432
Cytogenetics 15q21
MW 32.8 kDa
Summary protein phosphatase regulator protein; human homolog appears to be involved in histone acetylation [RGD, Feb 2006]
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SKU Description Size Price
RN213325 Setl1 (untagged ORF) - Rat SET translocation-like 1 (Setl1), (10 ug) 10 ug
$330.00
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RR213325L4 Lenti ORF clone of Setl1 (mGFP-tagged ORF) - Rat SET translocation-like 1 (Setl1), (10 ug) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.