Il1rn (NM_022194) Rat Tagged ORF Clone
SKU
RR213138
Il1rn (Myc-DDK-tagged ORF) - Rat interleukin 1 receptor antagonist (Il1rn), (10 ug)
-
TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
Click here to learn more.
Product Data | |
Target Symbol | Il1rn |
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Synonyms | IL-1ra |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RR213138 representing NM_022194
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATCTGCAGGGGACCTTACAGTCACCTAATCTCTCTCCTTCTCATCCTTCTGTTTCGTTCAGAGT CAGCTGGCCACCCTGCTGGGAAAAGACCCTGCAAGATGCAAGCCTTCAGAATCTGGGATACTAACCAGAA GACCTTCTACCTGAGGAACAACCAGCTCATTGCTGGGTACTTACAAGGACCAAATACCAAACTAGAAGAA AAGATAGACATGGTGCCTATTGACTTTCGGAATGTGTTCTTGGGCATCCACGGGGGCAAGCTGTGCCTGT CTTGTGTCAAGTCTGGAGATGACACCAAGCTCCAGCTGGAGGAGGTTAACATCACTGATCTGAACAAGAA CAAAGAAGAAGACAAGCGCTTTACCTTCATCCGCTCCGAGACAGGCCCTACCACCAGCTTCGAATCACTT GCCTGTCCAGGATGGTTCCTCTGCACAACACTAGAGGCTGATCATCCCGTGAGCCTCACCAACACACCAA AAGAGCCCTGTACAGTCACAAAGTTCTACTTCCAGGAAGACCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RR213138 representing NM_022194
Red=Cloning site Green=Tags(s) MEICRGPYSHLISLLLILLFRSESAGHPAGKRPCKMQAFRIWDTNQKTFYLRNNQLIAGYLQGPNTKLEE KIDMVPIDFRNVFLGIHGGKLCLSCVKSGDDTKLQLEEVNITDLNKNKEEDKRFTFIRSETGPTTSFESL ACPGWFLCTTLEADHPVSLTNTPKEPCTVTKFYFQEDQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022194 |
ORF Size | 534 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_022194.2, NP_071530.1 |
RefSeq Size | 1942 bp |
RefSeq ORF | 537 bp |
Locus ID | 60582 |
UniProt ID | P25086 |
Cytogenetics | 3p13 |
MW | 20.7 kDa |
Summary | binds to IL-1 receptor, blocking the binding of IL-1 alpha and -beta [RGD, Feb 2006] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RN213138 | Il1rn (untagged ORF) - Rat interleukin 1 receptor antagonist (Il1rn), (10 ug) | 10 ug |
$480.00
|
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RR213138L3 | Lenti ORF clone of Il1rn (Myc-DDK-tagged ORF) - Rat interleukin 1 receptor antagonist (Il1rn), (10 ug) | 10 ug |
$750.00
|
|
RR213138L4 | Lenti ORF clone of Il1rn (mGFP-tagged ORF) - Rat interleukin 1 receptor antagonist (Il1rn), (10 ug) | 10 ug |
$750.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.