Gclm (NM_017305) Rat Tagged ORF Clone
Lenti ORF clone of Gclm (mGFP-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)
|Type||Rat Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Mammalian Cell Selection||Puromycin|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||822 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1524 bp|
|RefSeq ORF||825 bp|
|Gene Summary||an integral part of the enzyme gamma-glutamylcysteine synthetase, which catalyzes the first step of glutathione (GSH) synthesis [RGD, Feb 2006]|
|cDNA Clone Resources|
|RN210532||Gclm (untagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)||
|RR210532||Gclm (Myc-DDK-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)||
|RR210532L3||Lenti ORF clone of Gclm (Myc-DDK-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)||