Gclm (NM_017305) Rat Untagged Clone

CAT#: RN210532

Gclm (untagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gclm
• Synonyms: Glclr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN210532 representing NM_017305
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGACAGCCGCGCGGCCGGAGCACTTCTGGCGCGGGCCAGCACCCTGCACCTGCAGACCGGGA
  ACCTGCTCAACTGGGGCCGCCTGCGGAAAAAGTGTCCGTCCACGCACAGCGAGGAGCTTCGAGACTGTAT
  CCAAAAGACCTTGAATGAATGGAGCTCCCAAATCAGCCCTGATTTGGTCAGGGAGTTTCCAGATGTTTTG
  GAATGTACCATGTCCCATGCAGTGGAAAAGATAAACCCTGATGAAAGAGAAGAAATGAAAGTTTCTGCTA
  AACTGTTCATTGTAGGATCGAATTCTTCATCATCAACTAGAAATGCAGTTGACATGGCATGCTCAGTCCT
  TGGAGTTGCACAGCTGGACTCTGTCATCATGGCTTCCCCTCCAATTGAAGATGGAGTTAATCTTTCCTTG
  GAGCATTTGCAGCCTTACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAGAAGATTGTTGCTATAGGCA
  CCTCGGATCTAGACAAAACACAGTTGGAGCAGCTGTACCAGTGGGCACAGGTAAAACCCAATAGTAATCA
  AGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTGACTGCATTTGCTAAACAGTTTGACATACAG
  CTACTGACTCACAATGACCCAAAAGAACTGCTCTCTGAGGCAAGTTTCCAGGAAGCTCTTCAAGAAAGCA
  TCCCTGACATTGAAGCCCAGGAGTGGGTGCCACTGTGGCTGCTGAGGTACTCGGTCATCGTGAAAAGCAG
  AGGAATCATCAAGTCAAAAGGATACATTTTGCAAGCCAAAAGAAAGGGTTCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_017305
• Insert Size: 825 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017305.2, NP_059001.1
• RefSeq Size: 1524 bp
• RefSeq ORF: 825 bp
• Locus ID: 29739
• UniProt ID: P48508
• Cytogenetics: 2q41
• Gene Summary: an integral part of the enzyme gamma-glutamylcysteine synthetase, which catalyzes the first step of glutathione (GSH) synthesis [RGD, Feb 2006]