Gclm (NM_017305) Rat Tagged ORF Clone

CAT#: RR210532

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  • TrueORF®

Gclm (Myc-DDK-tagged ORF) - Rat glutamate cysteine ligase, modifier subunit (Gclm), (10 ug)



  "NM_017305" in other vectors (3)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 473.00


Availability*
4 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Gclm
Synonyms Glclr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR210532 representing NM_017305
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCACCGACAGCCGCGCGGCCGGAGCACTTCTGGCGCGGGCCAGCACCCTGCACCTGCAGACCGGGA
ACCTGCTCAACTGGGGCCGCCTGCGGAAAAAGTGTCCGTCCACGCACAGCGAGGAGCTTCGAGACTGTAT
CCAAAAGACCTTGAATGAATGGAGCTCCCAAATCAGCCCTGATTTGGTCAGGGAGTTTCCAGATGTTTTG
GAATGTACCATGTCCCATGCAGTGGAAAAGATAAACCCTGATGAAAGAGAAGAAATGAAAGTTTCTGCTA
AACTGTTCATTGTAGGATCGAATTCTTCATCATCAACTAGAAATGCAGTTGACATGGCATGCTCAGTCCT
TGGAGTTGCACAGCTGGACTCTGTCATCATGGCTTCCCCTCCAATTGAAGATGGAGTTAATCTTTCCTTG
GAGCATTTGCAGCCTTACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAGAAGATTGTTGCTATAGGCA
CCTCGGATCTAGACAAAACACAGTTGGAGCAGCTGTACCAGTGGGCACAGGTAAAACCCAATAGTAATCA
AGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTGACTGCATTTGCTAAACAGTTTGACATACAG
CTACTGACTCACAATGACCCAAAAGAACTGCTCTCTGAGGCAAGTTTCCAGGAAGCTCTTCAAGAAAGCA
TCCCTGACATTGAAGCCCAGGAGTGGGTGCCACTGTGGCTGCTGAGGTACTCGGTCATCGTGAAAAGCAG
AGGAATCATCAAGTCAAAAGGATACATTTTGCAAGCCAAAAGAAAGGGTTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR210532 representing NM_017305
Red=Cloning site Green=Tags(s)

MGTDSRAAGALLARASTLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVL
ECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRNAVDMACSVLGVAQLDSVIMASPPIEDGVNLSL
EHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQ
LLTHNDPKELLSEASFQEALQESIPDIEAQEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRKGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017305
ORF Size 822 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017305.2, NP_059001.1
RefSeq Size 1524 bp
RefSeq ORF 825 bp
Locus ID 29739
UniProt ID P48508
Cytogenetics 2q41
MW 30.5 kDa
Gene Summary an integral part of the enzyme gamma-glutamylcysteine synthetase, which catalyzes the first step of glutathione (GSH) synthesis [RGD, Feb 2006]

Other Versions

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