Lhb (NM_012858) Rat Tagged ORF Clone

SKU
RR200432
Lhb (Myc-DDK-tagged ORF) - Rat luteinizing hormone beta (Lhb), transcript variant 1, (10 ug)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$289.00
4 Weeks*
Specifications
Product Data
Type Rat Tagged ORF Clone
Target Symbol Lhb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_012858
ORF Size 423 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012858.2, NP_036990.1
RefSeq Size 533 bp
RefSeq ORF 426 bp
Locus ID 25329
UniProt ID P01230
Cytogenetics 1q22
MW 15.2 kDa
Summary subunit of luteinizing hormone; involved in spermatogenesis and oogenesis [RGD, Feb 2006]
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SKU Description Size Price
RN200432 Lhb (untagged ORF) - Rat luteinizing hormone beta (Lhb), transcript variant 1, (10 ug) 10 ug
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RR200432L4 Lenti ORF clone of Lhb (mGFP-tagged ORF) - Rat luteinizing hormone beta (Lhb), transcript variant 1, (10 ug) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.