Gar1 (NM_001024306) Rat Untagged Clone

SKU RN211245

Gar1 (untagged ORF) - Rat nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) (Nola1), (10 ug)

Product Data

• Type: Rat Untagged Clone
• Target Symbol: Gar1
• Synonyms: Nola1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211245 representing NM_001024306; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTTTCCGAGGCGGAGGTCGCGGAGGCTTTAATCGCGGTGGTGGAGGTGGAGGCTTCAATCGTGGCG; GCGGCAGCAACAACCACTTCCGAGGGGGCGGCGGCGGTGGTGGCGGCGGCGGCAATTTCAGGGGCGGCGG; CCGAGGAGGATTTGGACGAGGGGGCGGTCGTGGAGGCTTCAATAAATTCCAAGATCAAGGGCCTCCAGAA; CGAGTCGTCTTATTAGGAGAGTTCATGCATCCCTGTGAAGATGACATCGTTTGTAAATGTACCACAGAAG; AAAACAAGGTGCCTTACTTCAATGCTCCTGTTTATTTAGAAAACAAAGAACAAATCGGGAAAGTGGATGA; AATATTTGGACAACTTAGAGATTTTTATTTTTCAGTTAAATTGTCAGAAAACATGAAGGCATCTTCTTTT; AAAAAGCTACAGAAGTTTTACATAGACCCATATAAGCTGCTGCCACTGCAGAGGTTTCTGCCTCGGCCTC; CTGGTGAGAAAGGACCTCCCAGAGGTGGTGGCGGTGGAGGTGGTGGTGGCAGGGGAGGTCGAGGAGGAGG; AAGAGGAGGCGGTGGCCGAGGTGGTGGAAGAGGTGGTGGTTTTAGAGGTGGAAGAGGAGGAGGTGGGGGC; TTCAGAGGAGGAAGAGGAGGAGGCGGCGGATTCCGAGGAAGGGGACATTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001024306
• Insert Size: 681 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001024306.1, NP_001019477.1
• RefSeq Size: 1386 bp
• RefSeq ORF: 681 bp
• Locus ID: 499709
• UniProt ID: Q6AYA1
• Cytogenetics: 2q43
• Summary: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (By similarity).[UniProtKB/Swiss-Prot Function]