KAT6B (NM_001256469) Human Tagged ORF Clone

SKU RG235335

KAT6B (tGFP-tagged) - Homo sapiens K(lysine) acetyltransferase 6B (KAT6B), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: KAT6B
• Synonyms: GTPTS; MORF; MOZ2; MYST4; qkf; querkopf; ZC2HC6B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG235335 representing NM_001256469; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTAAAACTTGCAAACCCACTTTATACAGAGTGGATTCTTGAAGCTATACAGAAAATAAAAAAGCAAA; AGCAAAGGCCCTCTGAAGAGAGAATCTGCCATGCGGTCAGTACTTCCCATGGGTTGGATAAGAAGACAGT; CTCTGAACAGCTGGAACTCAGTGTTCAGGATGGCTCAGTTCTCAAAGTCACCAACAAAGGCCTTGCCTCC; TATAAGGACCCAGACAACCCTGGGCGCTTTTCATCAGTTAAACCAGGCACTTTTCCTAAGTCAGCCAAGG; GGTCTAGAGGATCATGTAATGATCTCCGCAATGTGGATTGGAATAAACTTTTAAGGAGAGCAATTGAAGG; ACTTGAGGAGCCGAATGGCTCCTCCCTGAAGAACATAGAGAAGTATCTCAGAAGTCAAAGTGATCTCACA; AGCACCACCAACAACCCAGCCTTTCAGCAGCGGCTGCGACTGGGGGCCAAACGCGCTGTGAATAATGGGA; GGTTACTGAAAGACGGACCGCAGTACAGGGTCAATTATGGGAGCTTAGATGGCAAAGGGGCACCTCAGTA; TCCCAGTGCATTCCCATCCTCGCTCCCACCTGTCAGCCTTCTACCCCATGAGAAAGACCAGCCCCGTGCT; GATCCCATTCCAATATGTAGCTTCTGTTTGGGGACTAAAGAATCAAATCGTGAAAAGAAACCAGAAGAAC; TCCTCTCTTGTGCAGATTGTGGCAGTAGTGGACACCCATCCTGTTTGAAATTTTGTCCTGAATTAACAAC; AAATGTAAAGGCCTTAAGGTGGCAGTGCATCGAATGCAAGACATGCAGTGCCTGTAGAGTCCAAGGCAGA; AATGCTGATAATATGCTTTTTTGTGATTCCTGTGATAGAGGATTTCATATGGAATGCTGTGACCCACCAC; TTTCCAGAATGCCAAAAGGGATGTGGATTTGCCAAGTCTGCAGACCAAAGAAAAAGGGAAGAAAACTACT; TCATGAGAAAGCTGCACAAATAAAACGACGATATGCAAAACCCATTGGACGACCGAAAAATAAATTAAAG; CAACGATTGTTGTCTGTAACCAGTGATGAAGGATCCATGAATGCATTCACAGGAAGGGGGTCACCTGATA; CTGAAATAAAAATAAACATCAAACAAGAAAGTGCAGATGTAAATGTGATTGGAAACAAGGATGTCGTTAC; TGAAGAGGATTTGGATGTTTTTAAGCAGGCCCAGGAACTTTCTTGGGAGAAAATAGAGTGTGAGAGTGGG; GTGGAAGACTGTGGCCGGTACCCTTCTGTGATTGAATTTGGTAAATATGAAATCCAAACCTGGTACTCCT; CGCCTTACCCACAGGAATATGCAAGATTACCAAAGCTTTACCTGTGTGAATTCTGTCTTAAATATATGAA; AAGTAAAAATATTTTGCTAAGACACTCCAAGAAGTGTGGATGGTTTCATCCTCCAGCAAATGAAATTTAC; CGAAGGAAAGACCTTTCAGTATTTGAGGTTGATGGGAATATGAGCAAAATTTATTGCCAAAACCTTTGCT; TGTTAGCCAAGCTCTTCCTGGACCACAAAACGTTGTATTATGATGTCGAGCCATTCCTTTTTTATGTCCT; TACAAAAAATGATGAAAAGGGCTGTCATCTGGTTGGATACTTCTCTAAGGAAAAGCTTTGCCAGCAGAAG; TATAATGTCTCCTGCATAATGATCATGCCCCAGCACCAAAGGCAAGGATTTGGACGGTTTCTCATTGATT; TCAGCTATTTGCTTTCTAGAAGAGAAGGCCAAGCAGGGTCTCCTGAAAAGCCTCTCTCCGATCTGGGCCG; TCTCTCCTACCTGGCATATTGGAAGAGCGTCATCTTGGAGTATCTCTACCACCACCATGAGAGGCACATC; AGCATCAAGGCAATTAGCAGAGCGACGGGCATGTGCCCACATGACATTGCCACCACTCTGCAGCACCTCC; ACATGATCGACAAGAGAGATGGCAGATTTGTCATCATTAGACGGGAAAAGTTGATATTGAGCCACATGGA; AAAGCTGAAAACCTGTTCCAGAGCCAATGAACTTGATCCAGACAGTCTGAGGTGGACCCCAATTTTAATT; TCTAATGCTGCAGTGTCTGAAGAAGAGCGAGAAGCTGAGAAAGAGGCTGAGCGGCTAATGGAACAAGCTA; GCTGCTGGGAGAAGGAGGAACAAGAAATCCTGTCAACTAGAGCTAACAGTAGGCAATCACCTGCAAAAGT; ACAATCGAAAAATAAATATTTGCATTCCCCGGAGAGCCGGCCAGTCACAGGGGAGCGAGGGCAGCTGCTG; GAGCTGTCTAAAGAGAGCAGTGAAGAAGAAGAGGAGGAGGAGGACGAGGAGGAGGAAGAAGAGGAGGAAG; AAGAGGAAGAGGATGAAGAGGAGGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGAAAATATTCAAAGCTC; TCCCCCAAGATTGACGAAACCACAGTCAGTTGCCATAAAGAGAAAGAGGCCTTTTGTACTAAAGAAGAAA; AGGGGTCGTAAACGCAGGAGGATCAACAGCAGTGTAACAACAGAGACCATTTCAGAGACGACAGAAGTAC; TGAATGAGCCCTTTGACAACTCAGATGAAGAGAGGCCAATGCCACAGCTGGAGCCTACCTGTGAGATTGA; AGTGGAGGAAGATGGCAGGAAGCCAGTCCTGAGAAAAGCATTCCAGCATCAGCCTGGGAAGAAAAGACAA; ACAGAGGAAGAGGAAGGAAAAGACAATCATTGCTTCAAGAATGCTGACCCTTGTAGAAACAATATGAATG; ATGATTCAAGTAACTTGAAAGAAGGCAGTAAAGACAATCCCGAACCTCTAAAGTGCAAACAAGTGTGGCC; AAAAGGAACAAAGCGCGGTCTATCTAAGTGGAGGCAAAACAAAGAGAGGAAGACCGGATTTAAACTGAAT; TTGTACACCCCGCCAGAAACACCCATGGAGCCTGACGAGCAGGTAACAGTGGAAGAACAGAAGGAGACTT; CAGAAGGAAAAACCAGCCCCAGTCCCATCAGGATTGAGGAGGAGGTCAAGGAAACTGGGGAAGCCCTGTT; GCCTCAAGAGGAAAACAGAAGGGAAGAAACATGTGCCCCTGTAAGTCCAAACACATCACCAGGTGAAAAA; CCAGAAGATGATCTCATCAAACCTGAGGAAGAGGAAGAGGAGGAGGAGGAGGAAGAGGAAGAAGAGGAAG; AAGAGGAAGGGGAAGAAGAAGAAGGAGGAGGAAATGTAGAAAAAGATCCAGATGGTGCTAAAAGCCAAGA; AAAAGAGGAACCAGAAATCTCCACGGAAAAAGAAGACTCTGCACGTTTGGATGATCACGAAGAGGAGGAG; GAAGAGGATGAAGAGCCATCCCACAACGAGGACCATGATGCCGATGACGAGGATGACAGCCACATGGAGT; CTGCCGAAGTGGAGAAGGAAGAGCTGCCCAGAGAAAGCTTCAAAGAAGTACTGGAAAACCAGGAGACTTT; TTTAGACCTTAATGTGCAGCCTGGTCACTCGAACCCAGAGGTCTTAATGGACTGTGGCGTCGACCTGACA; GCTTCTTGTAACAGTGAGCCCAAGGAGCTTGCTGGGGACCCTGAAGCTGTACCCGAATCTGACGAGGAGC; CACCCCCAGGAGAACAGGCACAGAAGCAGGACCAAAAGAACAGCAAGGAAGTCGATACAGAGTTCAAAGA; GGGAAACCCAGCAACCATGGAAATCGACTCTGAGACTGTCCAGGCCGTTCAGTCTTTGACCCAGGAGAGC; AGCGAACAGGACGACACCTTTCAGGATTGTGCCGAGACTCAAGAGGCCTGTAGAAGCCTACAGAACTACA; CCCGTGCAGACCAAAGTCCACAGATTGCCACCACGCTCGACGATTGCCAACAGTCGGACCACAGTAGCCC; AGTTTCATCCGTCCACTCCCATCCTGGCCAGTCCGTACGTTCTGTCAACAGCCCAAGTGTCCCTGCTCTG; GAAAACAGCTACGCCCAAATCAGCCCAGATCAAAGTGCCATCTCAGTGCCATCTCTGCAGAACATGGAAA; CCAGTCCCATGATGGATGTCCCATCAGTTTCAGATCATTCACAGCAAGTCGTAGACAGTGGATTTAGTGA; CCTGGGCAGTATCGAGAGCACAACTGAGAACTACGAAAACCCAAGCAGCTACGATTCTACTATGGGAGGC; AGCATCTGTGGAAACGGCTCTTCACAGAACAGCTGCTCCTATAGCAACCTCACCTCCAGCAGTCTGACAC; AGAGCAGCTGTGCTGTCACCCAGCAGATGTCCAACATCAGCGGGAGCTGCAGCATGCTGCAGCAAACCAG; CATCAGCTCCCCTCCGACCTGCAGCGTCAAGTCTCCTCAAGGCTGTGTGGTGGAGAGGCCTCCGAGCAGC; AGCCAGCAGCTGGCTCAGTGCAGCATGGCTGCTAACTTCACCCCACCCATGCAGCTGGCTGAAATCCCCG; AGACGAGCAACGCCAACATTGGCTTATACGAGCGAATGGGTCAGAGTGATTTTGGGGCTGGGCATTACCC; GCAGCCGTCAGCCACCTTCAGCCTTGCCAAACTGCAGCAGTTAACTAATACACTTATTGATCATTCATTG; CCTTACAGCCATTCCGCTGCTGTGACTTCCTATGCAAACAGTGCCTCTTTGTCCACACCATTAAGTAACA; CAGGGCTTGTTCAACTTTCTCAGTCTCCACACTCCGTCCCTGGGGGACCCCAAGCACAAGCTACCATGAC; CCCACCCCCCAACCTGACTCCTCCTCCAATGAATCTGCCGCCGCCTCTTTTGCAACGGAACATGGCTGCA; TCAAATATTGGCATCTCTCACAGCCAAAGACTGCAAACCCAGATTGCCAGCAAGGGCCACATCTCCATGA; GAACCAAGTCAGCGTCTCTGTCACCAGCCGCTGCCACCCATCAGTCACAAATCTATGGGCGCTCCCAGAC; TGTAGCCATGCAGGGTCCTGCACGGACTTTAACGATGCAAAGAGGCATGAACATGAGTGTGAACCTGATG; CCAGCGCCAGCCTACAATGTCAACTCTGTGAACATGAACATGAACACTCTCAACGCCATGAATGGGTACA; GCATGTCCCAGCCAATGATGAACAGTGGCTACCACAGCAATCATGGCTATATGAATCAAACGCCCCAATA; CCCTATGCAGATGCAGATGGGCATGATGGGCACCCAGCCATATGCCCAGCAGCCAATGCAGACCCCACCC; CACGGTAACATGATGTACACGGCCCCCGGACATCACGGCTACATGAACACAGGCATGTCCAAACAGTCTC; TCAATGGCTCCTACATGAGAAGG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG235335 representing NM_001256469; Red=Cloning site Green=Tags(s); ; MVKLANPLYTEWILEAIQKIKKQKQRPSEERICHAVSTSHGLDKKTVSEQLELSVQDGSVLKVTNKGLAS; YKDPDNPGRFSSVKPGTFPKSAKGSRGSCNDLRNVDWNKLLRRAIEGLEEPNGSSLKNIEKYLRSQSDLT; STTNNPAFQQRLRLGAKRAVNNGRLLKDGPQYRVNYGSLDGKGAPQYPSAFPSSLPPVSLLPHEKDQPRA; DPIPICSFCLGTKESNREKKPEELLSCADCGSSGHPSCLKFCPELTTNVKALRWQCIECKTCSACRVQGR; NADNMLFCDSCDRGFHMECCDPPLSRMPKGMWICQVCRPKKKGRKLLHEKAAQIKRRYAKPIGRPKNKLK; QRLLSVTSDEGSMNAFTGRGSPDTEIKINIKQESADVNVIGNKDVVTEEDLDVFKQAQELSWEKIECESG; VEDCGRYPSVIEFGKYEIQTWYSSPYPQEYARLPKLYLCEFCLKYMKSKNILLRHSKKCGWFHPPANEIY; RRKDLSVFEVDGNMSKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVLTKNDEKGCHLVGYFSKEKLCQQK; YNVSCIMIMPQHQRQGFGRFLIDFSYLLSRREGQAGSPEKPLSDLGRLSYLAYWKSVILEYLYHHHERHI; SIKAISRATGMCPHDIATTLQHLHMIDKRDGRFVIIRREKLILSHMEKLKTCSRANELDPDSLRWTPILI; SNAAVSEEEREAEKEAERLMEQASCWEKEEQEILSTRANSRQSPAKVQSKNKYLHSPESRPVTGERGQLL; ELSKESSEEEEEEEDEEEEEEEEEEEEDEEEEEEEEEEEEEENIQSSPPRLTKPQSVAIKRKRPFVLKKK; RGRKRRRINSSVTTETISETTEVLNEPFDNSDEERPMPQLEPTCEIEVEEDGRKPVLRKAFQHQPGKKRQ; TEEEEGKDNHCFKNADPCRNNMNDDSSNLKEGSKDNPEPLKCKQVWPKGTKRGLSKWRQNKERKTGFKLN; LYTPPETPMEPDEQVTVEEQKETSEGKTSPSPIRIEEEVKETGEALLPQEENRREETCAPVSPNTSPGEK; PEDDLIKPEEEEEEEEEEEEEEEEEEGEEEEGGGNVEKDPDGAKSQEKEEPEISTEKEDSARLDDHEEEE; EEDEEPSHNEDHDADDEDDSHMESAEVEKEELPRESFKEVLENQETFLDLNVQPGHSNPEVLMDCGVDLT; ASCNSEPKELAGDPEAVPESDEEPPPGEQAQKQDQKNSKEVDTEFKEGNPATMEIDSETVQAVQSLTQES; SEQDDTFQDCAETQEACRSLQNYTRADQSPQIATTLDDCQQSDHSSPVSSVHSHPGQSVRSVNSPSVPAL; ENSYAQISPDQSAISVPSLQNMETSPMMDVPSVSDHSQQVVDSGFSDLGSIESTTENYENPSSYDSTMGG; SICGNGSSQNSCSYSNLTSSSLTQSSCAVTQQMSNISGSCSMLQQTSISSPPTCSVKSPQGCVVERPPSS; SQQLAQCSMAANFTPPMQLAEIPETSNANIGLYERMGQSDFGAGHYPQPSATFSLAKLQQLTNTLIDHSL; PYSHSAAVTSYANSASLSTPLSNTGLVQLSQSPHSVPGGPQAQATMTPPPNLTPPPMNLPPPLLQRNMAA; SNIGISHSQRLQTQIASKGHISMRTKSASLSPAAATHQSQIYGRSQTVAMQGPARTLTMQRGMNMSVNLM; PAPAYNVNSVNMNMNTLNAMNGYSMSQPMMNSGYHSNHGYMNQTPQYPMQMQMGMMGTQPYAQQPMQTPP; HGNMMYTAPGHHGYMNTGMSKQSLNGSYMRR; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256469
• ORF Size: 5343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001256469.1, NP_001243398.1
• RefSeq Size: 7633 bp
• RefSeq ORF: 5346 bp
• Locus ID: 23522
• UniProt ID: Q8WYB5
• Cytogenetics: 10q22.2
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is a histone acetyltransferase and component of the MOZ/MORF protein complex. In addition to its acetyltransferase activity, the encoded protein has transcriptional activation activity in its N-terminal end and transcriptional repression activity in its C-terminal end. This protein is necessary for RUNX2-dependent transcriptional activation and could be involved in brain development. Mutations have been found in patients with genitopatellar syndrome. A translocation of this gene and the CREBBP gene results in acute myeloid leukemias. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]