TRMT2B (NM_001167970) Human Tagged ORF Clone

SKU
RG230190
TRMT2B (tGFP-tagged) - Human TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) (TRMT2B), transcript variant 2
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

    Click here to learn more.

$669.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRMT2B
Synonyms CXorf34; dJ341D10.3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG230190 representing NM_001167970
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGCCTTAAGAGAAGAGTCCCACTGCACAGCCTCAGATACTTCATCTCCATGGTGGGTCTCTTCT
CCAAACCAGGACTGCTTCCCTGGTATGCCAGAAATCCACCAGGATGGTCACAGCTCTTTCTGGGCACAGT
ATGTAAGGGAGATTTCACCCGTGTGATAGCCACGAAATGTCAGAAAGGACAAAAAAGTCAGAAGAAACCA
AGCCATCTTGGACCACTAGATGGTTCCTGGCAGGAAAGGCTGGCTGATGTTGTGACACCACTCTGGAGGT
TGAGCTATGAAGAACAGCTCAAGGTGAAATTTGCAGCTCAGAAGAAAATTTTACAAAGACTAGAGTCTTA
CATCCAAATGCTCAATGGAGTCAGTGTGACAACGGCTGTACCCAAATCTGAGAGGCTCTCTTGTCTTCTC
CATCCTATTATACCCTCTCCTGTCATCAATGGTTACCGAAATAAGTCCACCTTCTCTGTGAACCGAGGTC
CAGATGGCAATCCAAAGACTGTGGGGTTCTACCTGGGAACTTGGAGAGATGGGAACGTTGTCTGTGTGCA
GTCTAATCATCTGAAAAACATCCCTGAGAAACACAGTCAAGTGGCGCAGTACTATGAAGTATTCCTTCGA
CAGTCTCCATTGGAGCCCTGCCTTGTATTTCATGAAGGTGGATACTGGCGTGAGCTCACAGTCCGCACCA
ATAGCCAAGGGCACACAATGGCTATCATCACTTTCCATCCCCAGAAATTAAGTCAGGAGGAGCTCCATGT
TCAGAAGGAGATTGTAAAGGAATTTTTCATCAGAGGTCCTGGAGCAGCCTGTGGCTTGACCTCACTTTAC
TTCCAGGAAAGTACCATGACCCGTTGCAGCCATCAGCAGTCTCCCTATCAGCTTCTGTTTGGGGAACCCT
ACATCTTTGAAGAACTTCTGAGCTTGAAGATCCGCATCTCTCCAGATGCCTTTTTCCAGATTAACACAGC
TGGTGCAGAGATGCTGTATCGGACTGTGGGGGAGCTGACTGGAGTGAACTCTGACACCATCCTTCTTGAC
ATCTGCTGTGGAACTGGTGTGATTGGCCTCTCTCTGGCTCAGCATACATCTCGGGTCCTTGGGATTGAAT
TGTTGGAGCAGGCAGTGGAGGATGCAAGATGGACTGCAGCCTTCAATGGCATCACCAACTCTGAATTTCA
TACTGGTCAAGCAGAGAAGATTTTGCCAGGGCTGCTAAAGTCAAAGGAAGATGGACAGTCAATTGTTGCT
GTGGTGAACCCAGCCCGTGCCGGACTGCATTACAAGGTGATTCAAGCCATTCGAAACTTCAGGGCCATCC
ACACGCTAGTTTTTGTTTCCTGCAAGCTCCATGGTGAATCCACTAGGAATGTCATTGAGCTGTGCTGTCC
TCCAGACCCTGCTAAGAAGCTCTTAGGCGAGCCCTTTGTCCTGCAGCAAGCTGTACCTGTGGATTTGTTC
CCTCACACCCCACATTGTGAGCTGGTGCTCCTCTTTACTCGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG230190 representing NM_001167970
Red=Cloning site Green=Tags(s)

MAGLKRRVPLHSLRYFISMVGLFSKPGLLPWYARNPPGWSQLFLGTVCKGDFTRVIATKCQKGQKSQKKP
SHLGPLDGSWQERLADVVTPLWRLSYEEQLKVKFAAQKKILQRLESYIQMLNGVSVTTAVPKSERLSCLL
HPIIPSPVINGYRNKSTFSVNRGPDGNPKTVGFYLGTWRDGNVVCVQSNHLKNIPEKHSQVAQYYEVFLR
QSPLEPCLVFHEGGYWRELTVRTNSQGHTMAIITFHPQKLSQEELHVQKEIVKEFFIRGPGAACGLTSLY
FQESTMTRCSHQQSPYQLLFGEPYIFEELLSLKIRISPDAFFQINTAGAEMLYRTVGELTGVNSDTILLD
ICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPGLLKSKEDGQSIVA
VVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCPPDPAKKLLGEPFVLQQAVPVDLF
PHTPHCELVLLFTR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001167970
ORF Size 1512 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001167970.1, NP_001161442.1
RefSeq Size 3181 bp
RefSeq ORF 1515 bp
Locus ID 79979
UniProt ID Q96GJ1
Cytogenetics Xq22.1
Protein Families Druggable Genome
Summary This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Nov 2009]
Write Your Own Review
You're reviewing:TRMT2B (NM_001167970) Human Tagged ORF Clone
Your Rating
SKU Description Size Price
RC230190 TRMT2B (Myc-DDK-tagged)-Human TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) (TRMT2B), transcript variant 2 10 ug
$289.00 MSRP $469.00 MSRP $469.00
RC230190L3 Lenti-ORF clone of TRMT2B (Myc-DDK-tagged)-Human TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) (TRMT2B), transcript variant 2 10 ug
$769.00
RC230190L4 Lenti-ORF clone of TRMT2B (mGFP-tagged)-Human TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) (TRMT2B), transcript variant 2 10 ug
$769.00
SC328828 TRMT2B (untagged)-Human TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) (TRMT2B) transcript variant 2 10 ug
$517.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.