TRMT2B (NM_001167970) Human Untagged Clone

SKU SC328828

TRMT2B (untagged)-Human TRM2 tRNA methyltransferase 2 homolog B (S. cerevisiae) (TRMT2B) transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRMT2B
• Synonyms: CXorf34; dJ341D10.3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001167970, the custom clone sequence may differ by one or more nucleotides; ATGGCAGGCCTTAAGAGAAGAGTCCCACTGCACAGCCTCAGATACTTCATCTCCATGGTG; GGTCTCTTCTCCAAACCAGGACTGCTTCCCTGGTATGCCAGAAATCCACCAGGATGGTCA; CAGCTCTTTCTGGGCACAGTATGTAAGGGAGATTTCACCCGTGTGATAGCCACGAAATGT; CAGAAAGGACAAAAAAGTCAGAAGAAACCAAGCCATCTTGGACCACTAGATGGTTCCTGG; CAGGAAAGGCTGGCTGATGTTGTGACACCACTCTGGAGGTTGAGCTATGAAGAACAGCTC; AAGGTGAAATTTGCAGCTCAGAAGAAAATTTTACAAAGACTAGAGTCTTACATCCAAATG; CTCAATGGAGTCAGTGTGACAACGGCTGTACCCAAATCTGAGAGGCTCTCTTGTCTTCTC; CATCCTATTATACCCTCTCCTGTCATCAATGGTTACCGAAATAAGTCCACCTTCTCTGTG; AACCGAGGTCCAGATGGCAATCCAAAGACTGTGGGGTTCTACCTGGGAACTTGGAGAGAT; GGGAACGTTGTCTGTGTGCAGTCTAATCATCTGAAAAACATCCCTGAGAAACACAGTCAA; GTGGCGCAGTACTATGAAGTATTCCTTCGACAGTCTCCATTGGAGCCCTGCCTTGTATTT; CATGAAGGTGGATACTGGCGTGAGCTCACAGTCCGCACCAATAGCCAAGGGCACACAATG; GCTATCATCACTTTCCATCCCCAGAAATTAAGTCAGGAGGAGCTCCATGTTCAGAAGGAG; ATTGTAAAGGAATTTTTCATCAGAGGTCCTGGAGCAGCCTGTGGCTTGACCTCACTTTAC; TTCCAGGAAAGTACCATGACCCGTTGCAGCCATCAGCAGTCTCCCTATCAGCTTCTGTTT; GGGGAACCCTACATCTTTGAAGAACTTCTGAGCTTGAAGATCCGCATCTCTCCAGATGCC; TTTTTCCAGATTAACACAGCTGGTGCAGAGATGCTGTATCGGACTGTGGGGGAGCTGACT; GGAGTGAACTCTGACACCATCCTTCTTGACATCTGCTGTGGAACTGGTGTGATTGGCCTC; TCTCTGGCTCAGCATACATCTCGGGTCCTTGGGATTGAATTGTTGGAGCAGGCAGTGGAG; GATGCAAGATGGACTGCAGCCTTCAATGGCATCACCAACTCTGAATTTCATACTGGTCAA; GCAGAGAAGATTTTGCCAGGGCTGCTAAAGTCAAAGGAAGATGGACAGTCAATTGTTGCT; GTGGTGAACCCAGCCCGTGCCGGACTGCATTACAAGGTGATTCAAGCCATTCGAAACTTC; AGGGCCATCCACACGCTAGTTTTTGTTTCCTGCAAGCTCCATGGTGAATCCACTAGGAAT; GTCATTGAGCTGTGCTGTCCTCCAGACCCTGCTAAGAAGCTCTTAGGCGAGCCCTTTGTC; CTGCAGCAAGCTGTACCTGTGGATTTGTTCCCTCACACCCCACATTGTGAGCTGGTGCTC; CTCTTTACTCGATAA;
• Restriction Sites: Please inquire
• ACCN: NM_001167970
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001167970.1, NP_001161442.1
• RefSeq Size: 3181 bp
• RefSeq ORF: 1515 bp
• Locus ID: 79979
• UniProt ID: Q96GJ1
• Cytogenetics: Xq22.1
• Protein Families: Druggable Genome
• Summary: This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Nov 2009]; Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 4 all encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.