SLC25A19 (NM_001126122) Human Tagged ORF Clone

SKU RG225460

SLC25A19 (tGFP-tagged) - Human solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 (SLC25A19), nuclear gene encoding mitochondrial protein, transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SLC25A19
• Synonyms: DNC; MCPHA; MUP1; THMD3; THMD4; TPC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225460 representing NM_001126122; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTTGGCTATGACCCCAAACCAGATGGCAGGAATAACACCAAGTTCCAGGTGGCAGTGGCTGGGTCTG; TGTCTGGACTTGTTACTCGGGCGCTGATCAGTCCCTTCGACGTCATCAAGATCCGTTTCCAGCTTCAGCA; TGAGCGCCTGTCTCGCAGTGACCCCAGCGCAAAGTACCATGGCATCCTCCAGGCCTCTAGGCAGATTCTG; CAGGAGGAGGGTCCGACAGCTTTCTGGAAAGGACACGTCCCAGCTCAGATTCTCTCCATAGGCTATGGAG; CTGTCCAATTCTTGTCATTTGAAATGCTGACGGAGCTGGTCCACAGAGGCAGCGTGTATGACGCCCGGGA; ATTCTCAGTGCACTTTGTATGTGGTGGCCTGGCTGCCTGTATGGCCACCCTCACTGTGCACCCCGTGGAT; GTTCTGCGCACCCGCTTTGCAGCTCAGGGTGAGCCCAAGGTCTATAATACGCTGCGCCACGCCGTGGGGA; CCATGTATAGGAGCGAAGGCCCCCAGGTTTTCTACAAAGGCTTGGCTCCCACCTTGATCGCCATCTTCCC; CTACGCCGGGCTGCAGTTCTCTTGCTACAGCTCCTTGAAGCACCTGTACAAGTGGGCCATACCAGCCGAA; GGAAAGAAAAATGAGAACCTCCAAAACCTGCTTTGTGGCAGTGGAGCTGGTGTCATCAGCAAGACCCTGA; CATATCCGCTGGACCTCTTCAAGAAGCGGCTACAGGTTGGAGGGTTTGAGCATGCCAGAGCTGCCTTTGG; CCAGGTACGGAGATACAAGGGCCTCATGGACTGTGCCAAGCAGGTGCTGCAAAAGGAAGGCGCCCTGGGC; TTCTTCAAGGGCCTGTCCCCCAGCTTGCTGAAGGCTGCCCTCTCCACAGGCTTCATGTTCTTCTCGTATG; AATTCTTCTGTAATGTCTTCCACTGCATGAACAGGACAGCCAGCCAGCGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225460 representing NM_001126122; Red=Cloning site Green=Tags(s); ; MVGYDPKPDGRNNTKFQVAVAGSVSGLVTRALISPFDVIKIRFQLQHERLSRSDPSAKYHGILQASRQIL; QEEGPTAFWKGHVPAQILSIGYGAVQFLSFEMLTELVHRGSVYDAREFSVHFVCGGLAACMATLTVHPVD; VLRTRFAAQGEPKVYNTLRHAVGTMYRSEGPQVFYKGLAPTLIAIFPYAGLQFSCYSSLKHLYKWAIPAE; GKKNENLQNLLCGSGAGVISKTLTYPLDLFKKRLQVGGFEHARAAFGQVRRYKGLMDCAKQVLQKEGALG; FFKGLSPSLLKAALSTGFMFFSYEFFCNVFHCMNRTASQR; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126122
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001126122.1, NP_001119594.1
• RefSeq Size: 1585 bp
• RefSeq ORF: 963 bp
• Locus ID: 60386
• UniProt ID: Q9HC21
• Cytogenetics: 17q25.1
• Protein Families: Druggable Genome
• Summary: This gene encodes a mitochondrial protein that is a member of the solute carrier family. Although this protein was initially thought to be the mitochondrial deoxynucleotide carrier involved in the uptake of deoxynucleotides into the matrix of the mitochondria, further studies have demonstrated that this protein instead functions as the mitochondrial thiamine pyrophosphate carrier, which transports thiamine pyrophosphates into mitochondria. Mutations in this gene cause microcephaly, Amish type, a metabolic disease that results in severe congenital microcephaly, severe 2-ketoglutaric aciduria, and death within the first year. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]