FBXL11 (KDM2A) (NM_012308) Human Tagged ORF Clone

SKU RG223440

KDM2A (tGFP-tagged) - Human lysine (K)-specific demethylase 2A (KDM2A), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FBXL11
• Synonyms: CXXC8; FBL7; FBL11; FBXL11; JHDM1A; LILINA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223440 representing NM_012308; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAACCCGAAGAAGAAAGGATTCGTTACAGCCAGAGATTGCGTGGTACCATGCGACGACGCTATGAAG; ATGATGGCATTTCAGATGATGAAATTGAAGGAAAAAGAACTTTTGACTTGGAAGAGAAACTGCACACCAA; CAAATATAATGCCAATTTTGTTACTTTTATGGAAGGAAAAGATTTTAATGTAGAGTATATTCAGCGGGGT; GGCTTGAGAGATCCTCTGATTTTCAAGAATTCTGATGGACTCGGAATAAAAATGCCGGATCCAGACTTCA; CTGTGAATGATGTCAAAATGTGTGTGGGGAGTCGTCGCATGGTGGATGTCATGGACGTGAACACACAGAA; AGGCATTGAAATGACCATGGCTCAGTGGACACGCTACTATGAGACCCCAGAGGAGGAGCGAGAGAAACTC; TATAATGTCATCAGCCTCGAGTTTAGCCACACCAGGCTGGAGAATATGGTGCAGAGGCCCTCCACGGTGG; ATTTCATTGACTGGGTAGACAACATGTGGCCAAGGCACTTGAAGGAAAGCCAGACTGAATCAACAAATGC; CATCTTGGAGATGCAGTACCCTAAAGTGCAGAAGTACTGTCTAATGAGTGTTCGAGGCTGCTATACTGAC; TTCCATGTGGACTTTGGTGGTACCTCTGTTTGGTATCACATCCATCAAGGGGGAAAGGTCTTCTGGCTCA; TCCCCCCTACAGCCCACAACCTGGAGCTGTACGAGAATTGGCTGCTGTCAGGGAAACAGGGAGACATCTT; TCTGGGTGACCGGGTATCAGATTGTCAGCGCATTGAGCTCAAGCAGGGCTATACCTTCGTCATTCCCTCA; GGCTGGATTCATGCTGTGTATACTCCTACAGACACATTAGTGTTTGGGGGCAATTTTTTGCATAGCTTCA; ACATCCCTATGCAGTTAAAAATATACAACATTGAAGATCGGACACGGGTTCCAAATAAGTTTCGCTATCC; ATTCTACTATGAGATGTGTTGGTATGTGTTGGAGCGCTATGTGTACTGCATAACCAACCGTTCCCACCTA; ACCAAGGAATTTCAGAAAGAGTCCCTCAGCATGGATTTGGAGTTAAATGGGTTGGAGTCTGGGAATGGGG; ATGAGGAAGCAGTGGATCGAGAACCCCGACGCTTGAGCAGCAGGCGTTCTGTCCTCACTAGCCCTGTAGC; GAATGGAGTCAACCTGGATTATGATGGACTGGGCAAAACCTGCCGAAGTCTTCCAAGTCTGAAGAAAACT; TTGGCTGGGGACTCATCTTCTGACTGTAGCCGGGGCTCCCACAATGGACAAGTGTGGGATCCCCAGTGTG; CTCCCCGAAAGGACAGGCAAGTGCATCTGACCCATTTTGAGCTTGAAGGCCTTCGCTGCCTTGTAGATAA; GTTGGAGTCTCTGCCACTGCACAAGAAATGTGTCCCCACAGGGATAGAAGATGAAGATGCTCTCATTGCT; GATGTAAAGATTTTGCTGGAGGAGCTTGCCAACAGCGATCCCAAGTTAGCCCTCACTGGAGTTCCTATAG; TACAGTGGCCAAAAAGGGATAAGCTTAAATTCCCCACTCGGCCAAAGGTGCGGGTTCCTACCATCCCCAT; TACGAAGCCTCACACTATGAAACCAGCTCCACGGTTAACACCTGTGAGGCCAGCTGCTGCCTCCCCGATT; GTGTCAGGAGCCAGACGGAGACGAGTGCGATGTCGAAAATGCAAAGCCTGTGTGCAAGGAGAGTGTGGTG; TTTGCCACTACTGCAGAGACATGAAGAAGTTTGGGGGGCCTGGACGCATGAAGCAGTCCTGTGTCCTCCG; ACAGTGCTTGGCACCCAGACTGCCTCACTCAGTCACATGTTCCCTCTGTGGAGAGGTGGATCAGAATGAA; GAGACACAAGACTTTGAGAAGAAACTCATGGAATGCTGTATCTGCAATGAGATTGTTCATCCTGGCTGCC; TCCAGATGGACGGAGAGGGGTTGCTTAACGAAGAATTGCCAAATTGCTGGGAATGTCCAAAGTGCTACCA; GGAGGACAGCTCGGAGAAAGCCCAGAAGCGGAAAATGGAAGAGAGTGACGAAGAAGCTGTGCAAGCCAAA; GTCCTGCGGCCCCTGCGGAGCTGCGATGAGCCTCTCACGCCCCCGCCTCATTCACCCACTTCCATGCTGC; AGCTCATCCATGACCCGGTTTCCCCCCGGGGTATGGTGACTCGGTCATCCCCTGGGGCTGGCCCCAGCGA; CCACCACAGTGCCAGCCGCGATGAGCGCTTCAAACGGCGGCAGTTGCTGCGGCTGCAGGCCACAGAGCGC; ACCATGGTACGGGAAAAGGAGAACAATCCCAGCGGCAAAAAGGAGCTGTCTGAAGTTGAGAAAGCCAAGA; TCCGGGGATCGTACCTCACTGTCACGCTACAGAGGCCCACCAAAGAGCTCCACGGGACATCCATTGTGCC; CAAGCTGCAGGCCATCACGGCCTCCTCTGCCAACCTTCGCCATTCCCCCCGTGTGCTAGTGCAGCACTGC; CCAGCCCGAACCCCCCAGCGTGGGGATGAGGAGGGGCTGGGGGGAGAGGAGGAGGAAGAGGAGGAGGAGG; AGGAGGAAGATGACAGTGCAGAGGAGGGGGGTGCAGCCAGGCTGAATGGCCGGGGCAGTTGGGCTCAGGA; TGGAGACGAAAGCTGGATGCAGCGGGAGGTCTGGATGTCTGTCTTCCGCTACCTCAGCCGCAGAGAACTT; TGTGAATGTATGCGAGTGTGCAAGACGTGGTATAAATGGTGCTGCGACAAGAGACTTTGGACAAAAATTG; ACTTGAGTAGGTGTAAGGCCATTGTGCCCCAGGCCCTCAGTGGCATCATCAAGAGGCAGCCAGTCAGCCT; TGACCTCAGTTGGACCAACATCTCTAAAAAGCAACTGACATGGCTCGTCAATAGGCTGCCAGGACTGAAA; GACCTCCTCCTAGCAGGCTGCTCCTGGTCTGCAGTCTCTGCCCTCAGCACCTCCAGCTGCCCCCTTCTCA; GGACCCTTGATCTTCGGTGGGCAGTAGGAATCAAGGACCCTCAAATTCGGGACTTGCTTACTCCACCGGC; TGATAAACCAGGTCAGGACAATCGCAGCAAGCTCCGGAACATGACCGACTTCCGGCTGGCAGGCCTTGAC; ATCACAGATGCCACGCTTCGCCTCATAATTCGCCACATGCCCCTCCTGTCTCGACTCGACCTCAGTCACT; GCAGCCACCTTACAGATCAGTCCTCCAATCTACTCACTGCTGTCGGGTCTTCCACTCGCTACTCTCTCAC; AGAGCTCAATATGGCAGGTTGCAATAAATTGACAGACCAGACCCTGATCTACCTACGGCGCATTGCCAAC; GTCACCTTGATCGACCTTCGAGGATGCAAGCAGATCACTCGAAAAGCCTGCGAGCACTTCATCTCAGACT; TGTCCATCAACAGCCTCTACTGCCTGTCTGACGAGAAGCTGATACAGAAGATCAGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223440 representing NM_012308; Red=Cloning site Green=Tags(s); ; MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLHTNKYNANFVTFMEGKDFNVEYIQRG; GLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKL; YNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTD; FHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPS; GWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYNIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHL; TKEFQKESLSMDLELNGLESGNGDEEAVDREPRRLSSRRSVLTSPVANGVNLDYDGLGKTCRSLPSLKKT; LAGDSSSDCSRGSHNGQVWDPQCAPRKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIA; DVKILLEELANSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPI; VSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNE; ETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSEKAQKRKMEESDEEAVQAK; VLRPLRSCDEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATER; TMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRHSPRVLVQHC; PARTPQRGDEEGLGGEEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRREL; CECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLK; DLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPADKPGQDNRSKLRNMTDFRLAGLD; ITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLIYLRRIAN; VTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012308
• ORF Size: 3486 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012308.1, NP_036440.1
• RefSeq Size: 6210 bp
• RefSeq ORF: 3489 bp
• Locus ID: 22992
• UniProt ID: Q9Y2K7
• Cytogenetics: 11q13.2
• Domains: F-box, JmjC, LRR_CC, PHD, zf-CXXC
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]