LIMD1 (NM_014240) Human Tagged ORF Clone

SKU RG222723

LIMD1 (tGFP-tagged) - Human LIM domains containing 1 (LIMD1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: LIMD1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222723 representing NM_014240; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATAAGTATGACGACCTGGGCCTGGAGGCCAGTAAATTCATCGAGGACCTGAACATGTATGAGGCCT; CTAAGGATGGGCTCTTCCGAGTGGACAAGGGTGCAGGCAACAACCCCGAGTTTGAGGAAACTCGCAGGGT; GTTCGCCACCAAGATGGCCAAAATCCACCTCCAGCAGCAGCAGCAGCAGCTCCTGCAGGAGGAGACTCTG; CCCAGGGGGAGTAGAGGCCCTGTCAATGGAGGGGGCCGCCTGGGCCCACAGGCCCGTTGGGAAGTTGTGG; GCAGCAAGCTGACTGTGGATGGTGCTGCCAAGCCTCCTCTTGCTGCCTCGACAGGGGCACCTGGGGCAGT; CACCACCCTCGCTGCTGGGCAGCCCCCGTACCCACCGCAGGAGCAGAGATCCAGGCCATACCTGCATGGC; ACGAGGCATGGCAGCCAGGACTGTGGTTCCAGGGAGAGCCTGGCGACTTCTGAGATGTCTGCTTTCCACC; AGCCAGGCCCCTGTGAGGATCCTTCCTGCCTCACTCATGGAGACTATTATGACAACCTCTCCTTGGCAAG; CCCAAAGTGGGGTGACAAACCAGGAGTGTCCCCCAGCATCGGCCTGAGTGTAGGGAGTGGGTGGCCTAGC; TCCCCGGGGAGTGACCCACCACTGCCCAAACCCTGCGGGGACCATCCCCTAAATCACCGACAGCTCTCCC; TGAGCTCCAGCAGGTCTTCTGAGGGTAGCCTCGGTGGTCAGAATAGTGGCATTGGTGGCCGCAGCAGCGA; GAAGCCAACAGGCCTTTGGTCCACTGCCTCCTCCCAGCGGGTGAGCCCTGGCCTGCCTTCCCCAAACTTG; GAGAACGGAGCACCAGCTGTGGGGCCTGTTCAGCCCAGGACTCCTTCTGTGTCAGCACCCTTGGCCCTGA; GCTGCCCCAGGCAAGGAGGTCTTCCAAGATCAAACTCGGGGCTGGGGGGTGAGGTTTCAGGTGTGATGTC; CAAACCCAATGTGGACCCCCAACCCTGGTTCCAGGATGGGCCCAAATCTTACCTTTCCAGTTCTGCCCCG; TCATCCTCGCCAGCTGGCCTGGACGGTTCACAGCAGGGTGCGGTCCCTGGGCTGGGGCCGAAGCCTGGCT; GCACAGACCTTGGCACTGGTCCCAAGCTCAGCCCCACCAGTCTTGTCCATCCAGTGATGTCCACCCTGCC; TGAGTTATCTTGTAAAGAGGGTCCCCTGGGCTGGTCTTCTGATGGTAGCCTGGGATCTGTGCTCCTGGAC; AGCCCCAGCTCCCCTAGGGTAAGGCTGCCCTGCCAGCCCCTCGTCCCAGGTCCTGAGCTGAGACCCTCTG; CTGCTGAGTTGAAATTAGAAGCCCTCACCCAACGTCTGGAGCGAGAGATGGATGCTCACCCGAAGGCTGA; TTACTTTGGAGCCTGTGTGAAATGCAGCAAAGGGGTGTTTGGGGCTGGCCAGGCCTGTCAGGCCATGGGG; AACCTCTACCATGACACATGCTTCACCTGTGCAGCTTGCAGCCGGAAGCTGAGAGGAAAAGCCTTTTATT; TTGTCAACGGCAAAGTGTTTTGTGAAGAAGACTTCCTGTACTCTGGTTTCCAGCAGTCGGCTGACAGGTG; TTTTCTTTGTGGACATCTGATCATGGACATGATCCTGCAAGCCCTGGGGAAGTCCTACCACCCCGGCTGT; TTCCGCTGTGTCATCTGTAATGAGTGTTTGGATGGGGTGCCCTTCACCGTGGACTCAGAGAACAAGATCT; ACTGTGTCCGAGATTACCACAAGGTGCTGGCCCCCAAGTGTGCAGCCTGTGGGCTTCCCATCCTTCCACC; TGAGGGCTCAGATGAGACCATCCGTGTCGTGTCCATGGACAGAGACTACCACGTGGAGTGTTACCACTGC; GAGGACTGTGGTCTGGAGCTCAATGATGAAGATGGCCACCGCTGTTATCCGCTGGAGGACCACCTGTTCT; GTCACTCCTGCCACGTGAAGAGGCTGGAGAAGAGACCCTCATCTACAGCCCTTCACCAGCACCACTTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222723 representing NM_014240; Red=Cloning site Green=Tags(s); ; MDKYDDLGLEASKFIEDLNMYEASKDGLFRVDKGAGNNPEFEETRRVFATKMAKIHLQQQQQQLLQEETL; PRGSRGPVNGGGRLGPQARWEVVGSKLTVDGAAKPPLAASTGAPGAVTTLAAGQPPYPPQEQRSRPYLHG; TRHGSQDCGSRESLATSEMSAFHQPGPCEDPSCLTHGDYYDNLSLASPKWGDKPGVSPSIGLSVGSGWPS; SPGSDPPLPKPCGDHPLNHRQLSLSSSRSSEGSLGGQNSGIGGRSSEKPTGLWSTASSQRVSPGLPSPNL; ENGAPAVGPVQPRTPSVSAPLALSCPRQGGLPRSNSGLGGEVSGVMSKPNVDPQPWFQDGPKSYLSSSAP; SSSPAGLDGSQQGAVPGLGPKPGCTDLGTGPKLSPTSLVHPVMSTLPELSCKEGPLGWSSDGSLGSVLLD; SPSSPRVRLPCQPLVPGPELRPSAAELKLEALTQRLEREMDAHPKADYFGACVKCSKGVFGAGQACQAMG; NLYHDTCFTCAACSRKLRGKAFYFVNGKVFCEEDFLYSGFQQSADRCFLCGHLIMDMILQALGKSYHPGC; FRCVICNECLDGVPFTVDSENKIYCVRDYHKVLAPKCAACGLPILPPEGSDETIRVVSMDRDYHVECYHC; EDCGLELNDEDGHRCYPLEDHLFCHSCHVKRLEKRPSSTALHQHHF; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014240
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014240.1, NP_055055.1
• RefSeq Size: 5067 bp
• RefSeq ORF: 2031 bp
• Locus ID: 8994
• UniProt ID: Q9UGP4
• Cytogenetics: 3p21.31
• Summary: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation.[UniProtKB/Swiss-Prot Function]