TEM8 (ANTXR1) (NM_032208) Human Tagged ORF Clone

SKU RG222030

ANTXR1 (tGFP-tagged) - Human anthrax toxin receptor 1 (ANTXR1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TEM8
• Synonyms: ATR; GAPO; TEM8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222030 representing NM_032208; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCACGGCGGAGCGGAGAGCCCTCGGCATCGGCTTCCAGTGGCTCTCTTTGGCCACTCTGGTGCTCA; TCTGCGCCGGGCAAGGGGGACGCAGGGAGGATGGGGGTCCAGCCTGCTACGGCGGATTTGACCTGTACTT; CATTTTGGACAAATCAGGAAGTGTGCTGCACCACTGGAATGAAATCTATTACTTTGTGGAACAGTTGGCT; CACAAATTCATCAGCCCACAGTTGAGAATGTCCTTTATTGTTTTCTCCACCCGAGGAACAACCTTAATGA; AACTGACAGAAGACAGAGAACAAATCCGTCAAGGCCTAGAAGAACTCCAGAAAGTTCTGCCAGGAGGAGA; CACTTACATGCATGAAGGATTTGAAAGGGCCAGTGAGCAGATTTATTATGAAAACAGACAAGGGTACAGG; ACAGCCAGCGTCATCATTGCTTTGACTGATGGAGAACTCCATGAAGATCTCTTTTTCTATTCAGAGAGGG; AGGCTAATAGGTCTCGAGATCTTGGTGCAATTGTTTACTGTGTTGGTGTGAAAGATTTCAATGAGACACA; GCTGGCCCGGATTGCGGACAGTAAGGATCATGTGTTTCCCGTGAATGACGGCTTTCAGGCTCTGCAAGGC; ATCATCCACTCAATTTTGAAGAAGTCCTGCATCGAAATTCTAGCAGCTGAACCATCCACCATATGTGCAG; GAGAGTCATTTCAAGTTGTCGTGAGAGGAAACGGCTTCCGACATGCCCGCAACGTGGACAGGGTCCTCTG; CAGCTTCAAGATCAATGACTCGGTCACACTCAATGAGAAGCCCTTTTCTGTGGAAGATACTTATTTACTG; TGTCCAGCGCCTATCTTAAAAGAAGTTGGCATGAAAGCTGCACTCCAGGTCAGCATGAACGATGGCCTCT; CTTTTATCTCCAGTTCTGTCATCATCACCACCACACACTGTTCTGACGGTTCCATCCTGGCCATCGCCCT; GCTGATCCTGTTCCTGCTCCTAGCCCTGGCTCTCCTCTGGTGGTTCTGGCCCCTCTGCTGCACTGTGATT; ATCAAGGAGGTCCCTCCACCCCCTGCCGAGGAGAGTGAGGAAGAAGATGATGATGGTCTGCCTAAGAAAA; AGTGGCCAACGGTAGACGCCTCTTATTATGGTGGGAGAGGCGTTGGAGGCATTAAAAGAATGGAGGTTCG; TTGGGGAGAAAAGGGCTCCACAGAAGAAGGTGCTAAGTTGGAAAAGGCAAAGAATGCAAGAGTCAAGATG; CCGGAGCAGGAATATGAATTCCCTGAGCCGCGAAATCTCAACAACAATATGCGTCGGCCTTCTTCCCCCC; GGAAGTGGTACTCTCCAATCAAGGGAAAACTCGATGCCTTGTGGGTCCTACTGAGGAAAGGATATGATCG; TGTGTCTGTGATGCGTCCACAGCCAGGAGACACGGGGCGCTGCATCAACTTCACCAGGGTCAAGAACAAC; CAGCCAGCCAAGTACCCACTCAACAACGCCTACCACACCTCCTCGCCGCCTCCTGCCCCCATCTACACTC; CCCCACCTCCTGCGCCCCACTGCCCTCCCCCGCCCCCCAGCGCCCCTACCCCTCCCATCCCGTCCCCACC; TTCCACCCTTCCCCCTCCTCCCCAGGCTCCACCTCCCAACAGGGCACCTCCTCCCTCCCGCCCTCCTCCA; AGGCCTTCTGTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222030 representing NM_032208; Red=Cloning site Green=Tags(s); ; MATAERRALGIGFQWLSLATLVLICAGQGGRREDGGPACYGGFDLYFILDKSGSVLHHWNEIYYFVEQLA; HKFISPQLRMSFIVFSTRGTTLMKLTEDREQIRQGLEELQKVLPGGDTYMHEGFERASEQIYYENRQGYR; TASVIIALTDGELHEDLFFYSEREANRSRDLGAIVYCVGVKDFNETQLARIADSKDHVFPVNDGFQALQG; IIHSILKKSCIEILAAEPSTICAGESFQVVVRGNGFRHARNVDRVLCSFKINDSVTLNEKPFSVEDTYLL; CPAPILKEVGMKAALQVSMNDGLSFISSSVIITTTHCSDGSILAIALLILFLLLALALLWWFWPLCCTVI; IKEVPPPPAEESEEEDDDGLPKKKWPTVDASYYGGRGVGGIKRMEVRWGEKGSTEEGAKLEKAKNARVKM; PEQEYEFPEPRNLNNNMRRPSSPRKWYSPIKGKLDALWVLLRKGYDRVSVMRPQPGDTGRCINFTRVKNN; QPAKYPLNNAYHTSSPPPAPIYTPPPPAPHCPPPPPSAPTPPIPSPPSTLPPPPQAPPPNRAPPPSRPPP; RPSV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032208
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032208.3
• RefSeq Size: 5909 bp
• RefSeq ORF: 1695 bp
• Locus ID: 84168
• UniProt ID: Q9H6X2
• Cytogenetics: 2p13.3
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]