UPF3B (NM_023010) Human Tagged ORF Clone

SKU RG221828

UPF3B (tGFP-tagged) - Human UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: UPF3B
• Synonyms: HUPF3B; MRX62; MRX82; MRXS14; RENT3B; UPF3BP1; UPF3BP2; UPF3BP3; Upf3p-X; UPF3X
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221828 representing NM_023010; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGGAAGAGAAGGAGCACAGGCCTAAGGAGAAGCGAGTAACCCTGTTAACCCCCGCCGGGGCCACAG; GCAGCGGTGGTGGGACCTCGGGGGACAGCTCCAAGGGGGAAGATAAGCAGGATCGCAACAAGGAGAAGAA; AGAAGCGCTGAGCAAGGTGGTAATTCGAAGATTACCTCCCACTTTGACCAAGGAGCAGCTTCAGGAACAT; CTTCAACCTATGCCTGAGCATGATTATTTTGAGTTTTTTTCTAATGATACGAGTTTGTATCCTCATATGT; ATGCCAGAGCATACATCAACTTTAAAAACCAAGAGGACATTATTTTGTTCAGGGATCGCTTTGATGGTTA; TGTATTCCTTGACAATAAAGGTCAGGAATATCCCGCTATAGTAGAATTTGCACCTTTTCAAAAAGCTGCA; AAAAAGAAGACTAAGAAAAGAGATACCAAAGTCGGGACTATCGATGATGATCCAGAATATAGAAAGTTTT; TGGAAAGTTATGCCACAGATAATGAGAAAATGACATCTACTCCAGAGACACTGCTAGAGGAAATAGAAGC; AAAAAATAGAGAATTAATAGCTAAAAAGACAACCCCACTTTTGAGCTTCCTGAAAAACAAGCAGAGAATG; AGAGAAGAAAAGAGAGAAGAAAGGAGGAGGAGAGAAATAGAAAGAAAAAGACAAAGAGAAGAAGAGAGGA; GGAAATGGAAAGAAGAAGAGAAACGAAAAAGGAAAGATATAGAAAAGCTAAAGAAGATAGACAGAATTCC; AGAAAGGGACAAATTAAAGGATGAACCAAAGATTAAGCTGCTCAAGAAGCCAGAAAAAGGAGATGAAAAA; GAATTGGACAAAAGAGAAAAAGCCAAGAAATTGGACAAAGAGAATCTCAGTGATGAAAGAGCCAGTGGGC; AAAGTTGTACATTGCCCAAGCGTTCTGATAGCGAACTTAAAGATGAAAAACCAAAGAGACCTGAAGATGA; GAGCGGCAGAGACTATAGGGAGAGGGAACGGGAATATGAACGAGATCAGGAGCACATACTTCGAGAAAGA; GAGAGGCTGAAGCGGCAAGAAGAAGAGCGCCGTAGGCAGAAGGAGCGCTATGAGAAAGAGAAGACTTTTA; AGAGAAAAGAAGAAGAAATGAAAAAAGAGAAAGACACACTTCGGGATAAAGGAAAGAAGGCTGAAAGTAC; AGAATCAATAGGCAGCTCAGAAAAAACTGAAAAGAAAGAAGAAGTGGTCAAGAGAGATCGAATAAGAAAC; AAGGATCGTCCAGCGATGCAGCTTTACCAACCAGGAGCTCGAAGCCGAAATCGACTCTGTCCCCCTGATG; ACAGCACCAAGTCTGGAGATTCAGCAGCAGAAAGGAAGCAGGAAAGTGGTATTAGCCATAGAAAAGAAGG; AGGAGAGGAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221828 representing NM_023010; Red=Cloning site Green=Tags(s); ; MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEH; LQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAA; KKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRM; REEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKLLKKPEKGDEK; ELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYREREREYERDQEHILRER; ERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTEKKEEVVKRDRIRN; KDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023010
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_023010.2, NP_075386.1
• RefSeq Size: 2342 bp
• RefSeq ORF: 1413 bp
• Locus ID: 65109
• UniProt ID: Q9BZI7
• Cytogenetics: Xq24
• Domains: Smg4_UPF3
• Summary: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]