DAZ2 (NM_001005786) Human Tagged ORF Clone

SKU RG221790

DAZ2 (tGFP-tagged) - Human deleted in azoospermia 2 (DAZ2), transcript variant 3

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DAZ2
• Synonyms: pDP1678
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221790 representing NM_001005786; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTGCTGCAAATCCTGAGACTCCAAACTCAACCATCTCCAGAGAGGCCAGCACCCAGTCTTCATCAG; CTGCAGCTAGCCAAGGCTGGGTGTTACCAGAAGGCAAAATCGTGCCAAACACTGTTTTTGTTGGTGGAAT; TGATGCTAGGATGGATGAAACTGAGATTGGAAGCTGCTTTGGTAGATACGGTTCAGTGAAAGAAGTGAAG; ATAATCACGAATCGAACTGGTGTGTCCAAAGGCTATGGATTTGTTTCGTTTGTTAATGACGTGGATGTCC; AGAAGATAGTAGGATCACAGATACATTTCCATGGTAAAAAGCTGAAGCTGGGCCCTGCAATCAGGAAACA; AAAGTTATGTGCTCGTCATGTGCAGCCACGTCCTTTGGTAGTTAATCCTCCTCCTCCACCACAGTTTCAG; AACGTCTGGCGGAATCCAAACACTGAAACCTACCTGCAGCCCCAAATCACGCCGAATCCTGTAACTCAGC; ACGTTCAGGCTTATTCTGCTTATCCACATTCACCAGGTCAGGTCATCACTGGATGTCAGTTGCTTGTATA; TAATTATCAGGAATATCCTACTTATCCCGATTCAGCATTTCAGGTCACCACTGGATATCAGTTGCCTGTA; TATAATTATCAGCCATTTCCTGCTTATCCAAGTTCACCATTTCAGGTCACTGCTGGATATCAGTTGCCTG; TATATAATTATCAGGCATTTCCTGCTTATCCAAGTTCACCATTTCAGGTCACCACTGGATATCAGTTGCC; TGTATATAATTATCAGGCATTTCCTGCTTATCCAAGTTCACCATTTCAGGTCACCACTGGATATCAGTTG; CCTGTATATAATTATCAGGCATTTCCTGCTTATCCAAGTTCACCATTTCAGGTCACCACTGGATATCAGT; TGCCTGTATATAATTATCAGGCATTTCCTGCTTATCCAAATTCAGCAGTTCAGGTCACCACTGGATATCA; GTTCCATGTATACAATTACCAGATGCCACCGCAGTGCCCTGTTGGGGAGCAAAGGAGAAATCTGTGGACC; GAAGCATACAAATGGTGGTATCTTGTCTGTTTAATCCAGAGAAGAGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221790 representing NM_001005786; Red=Cloning site Green=Tags(s); ; MSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVK; IITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLCARHVQPRPLVVNPPPPPQFQ; NVWRNPNTETYLQPQITPNPVTQHVQAYSAYPHSPGQVITGCQLLVYNYQEYPTYPDSAFQVTTGYQLPV; YNYQPFPAYPSSPFQVTAGYQLPVYNYQAFPAYPSSPFQVTTGYQLPVYNYQAFPAYPSSPFQVTTGYQL; PVYNYQAFPAYPSSPFQVTTGYQLPVYNYQAFPAYPNSAVQVTTGYQFHVYNYQMPPQCPVGEQRRNLWT; EAYKWWYLVCLIQRRD; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005786
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001005786.2, NP_001005786.2
• RefSeq Size: 3277 bp
• RefSeq ORF: 1101 bp
• Locus ID: 57055
• UniProt ID: Q13117
• Cytogenetics: Yq11.223
• Summary: This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]