Frizzled 2 (FZD2) (NM_001466) Human Tagged ORF Clone

SKU RG220921

FZD2 (tGFP-tagged) - Human frizzled family receptor 2 (FZD2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Frizzled 2
• Synonyms: fz-2; Fz2; fzE2; hFz2; OMOD2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220921 representing NM_001466; Red=Cloning site Blue=ORF Green=Tags(s); ; ; ; ATGCGGCCCCGCAGCGCCCTGCCCCGCCTGCTGCTGCCGCTGCTGCTGCTGCCCGCCGCCGGGCCGGCCC; AGTTCCACGGGGAGAAGGGCATCTCCATCCCGGACCACGGCTTCTGCCAGCCCATCTCCATCCCGCTGTG; CACGGACATCGCCTACAACCAGACCATCATGCCCAACCTTCTGGGCCACACGAACCAGGAGGACGCAGGC; CTAGAGGTGCACCAGTTCTATCCGCTGGTGAAGGTGCAGTGCTCGCCCGAACTGCGCTTCTTCCTGTGCT; CCATGTACGCACCCGTGTGCACCGTGCTGGAACAGGCCATCCCGCCGTGCCGCTCTATCTGTGAGCGCGC; GCGCCAGGGCTGCGAAGCCCTCATGAACAAGTTCGGTTTTCAGTGGCCCGAGCGCCTGCGCTGCGAGCAC; TTCCCGCGCCACGGCGCCGAGCAGATCTGCGTCGGCCAGAACCACTCCGAGGACGGAGCTCCCGCGCTAC; TCACCACCGCGCCGCCGCCGGGACTGCAGCCGGGTGCCGGGGGCACCCCGGGTGGCCCGGGCGGCGGCGG; CGCTCCCCCGCGCTACGCCACGCTGGAGCACCCCTTCCACTGCCCGCGCGTCCTCAAGGTGCCATCCTAT; CTCAGCTACAAGTTTCTGGGCGAGCGTGATTGTGCTGCGCCCTGCGAACCTGCGCGGCCCGATGGTTCCA; TGTTCTTCTCACAGGAGGAGACGCGTTTCGCGCGCCTCTGGATCCTCACCTGGTCGGTGCTGTGCTGCGC; TTCCACCTTCTTCACTGTCACCACGTACTTGGTAGACATGCAGCGCTTCCGCTACCCAGAGCGGCCTATC; ATTTTTCTGTCGGGCTGCTACACCATGGTGTCGGTGGCCTACATCGCGGGCTTCGTGCTCCAGGAGCGCG; TGGTGTGCAACGAGCGCTTCTCCGAGGACGGTTACCGCACGGTGGTGCAGGGCACCAAGAAGGAGGGCTG; CACCATCCTCTTCATGATGCTCTACTTCTTCAGCATGGCCAGCTCCATCTGGTGGGTCATCCTGTCGCTC; ACCTGGTTCCTGGCAGCCGGCATGAAGTGGGGCCACGAGGCCATCGAGGCCAACTCTCAGTACTTCCACC; TGGCCGCCTGGGCCGTGCCGGCCGTCAAGACCATCACCATCCTGGCCATGGGCCAGATCGACGGCGACCT; GCTGAGCGGCGTGTGCTTCGTAGGCCTCAACAGCCTGGACCCGCTGCGGGGCTTCGTGCTAGCGCCGCTC; TTCGTGTACCTGTTCATCGGCACGTCCTTCCTCCTGGCCGGCTTCGTGTCGCTCTTCCGCATCCGCACCA; TCATGAAGCACGACGGCACCAAGACCGAAAAGCTGGAGCGGCTCATGGTGCGCATCGGCGTCTTCTCCGT; GCTCTACACAGTGCCCGCCACCATCGTCATCGCTTGCTACTTCTACGAGCAGGCCTTCCGCGAGCACTGG; GAGCGCTCGTGGGTGAGCCAGCACTGCAAGAGCCTGGCCATCCCGTGCCCGGCGCACTACACGCCGCGCA; TGTCGCCCGACTTCACGGTCTACATGATCAAATACCTCATGACGCTCATCGTGGGCATCACGTCGGGCTT; CTGGATCTGGTCGGGCAAGACGCTGCACTCGTGGAGGAAGTTCTACACTCGCCTCACCAACAGCCGACAC; GGTGAGACCACCGTG; ;
  Protein Sequence

  >RG220921 representing NM_001466; Red=Cloning site Green=Tags(s); ; MRPRSALPRLLLPLLLLPAAGPAQFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAG; LEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEH; FPRHGAEQICVGQNHSEDGAPALLTTAPPPGLQPGAGGTPGGPGGGGAPPRYATLEHPFHCPRVLKVPSY; LSYKFLGERDCAAPCEPARPDGSMFFSQEETRFARLWILTWSVLCCASTFFTVTTYLVDMQRFRYPERPI; IFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVVQGTKKEGCTILFMMLYFFSMASSIWWVILSL; TWFLAAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNSLDPLRGFVLAPL; FVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHW; ERSWVSQHCKSLAIPCPAHYTPRMSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNSRH; GETTV; ;
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001466
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001466.4
• RefSeq Size: 3834 bp
• RefSeq ORF: 1698 bp
• Locus ID: 2535
• UniProt ID: Q14332
• Cytogenetics: 17q21.31
• Domains: FRI, Frizzled
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• MW: 63.6 kDa
• Summary: This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010]