Frizzled 2 (FZD2) (NM_001466) Human Untagged Clone

SKU SC127603

FZD2 (untagged)-Human frizzled family receptor 2 (FZD2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Frizzled 2
• Synonyms: fz-2; Fz2; fzE2; hFz2; OMOD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001466 edited; GAATTCGGCACGAGGGGAGAGAGGGCAGCAGCGCGCGGTGTCTCCGGCTGCTCAGTCCGA; CCGCGGCAAGCAAGCGGGCAGGCGCACCGCCCCCTCCCCCGCCCGGCCTCCCCAACTCTG; CGGCCGCGAGTAAAGTTTGCAAAGAGGCGCGGGAGGCGGCAGCCGCAGCGAGGAGGCGGC; GGGGAAGAAGCGCAGTCTCCGGGTTGGGGGCGGGGGCGGGGGGGGCGCCAAGGAGCCGGG; TGGGGGGCGGCGGCCAGCATGCGGCCCCGCAGCGCCCTGCCCCGCCTGCTGCTGCCGCTG; CTGCTGCTGCCCGCCGCCGGGCCGGCCCAGTTCCACGGGGAGAAGGGCATCTCCATCCCG; GACCACGGCTTCTGCCAGCCCATCTCCATCCCGCTGTGCACGGACATCGCCTACAACCAG; ACCATCATGCCCAACCTTCTGGGCCACACGAACCAGGAGGACGCAGGCCTAGAGGTGCAC; CAGTTCTATCCGCTGGTGAAGGTGCAGTGCTCGCCCGAACTGCGCTTCTTCCTGTGCTCC; ATGTACGCACCCGTGTGCACCGTGCTGGAACAGGCCATCCCGCCGTGCCGCTCTATCTGT; GAGCGCGCGCGCCAGGGCTGCGAAGCCCTCATGAACAAGTTCGGTTTTCAGTGGCCCGAG; CGCCTGCGCTGCGAGCACTTCCCGCGCCACGGCGCCGAGCAGATCTGCGTCGGCCAGAAC; CACTCCGAGGACGGAGCTCCCGCGCTACTCACCACCGCGCCGCCGCCGGGACTGCAGCCG; GGTGCCGGGGGCACCCCGGGTGGCCCGGGCGGCGGCGGCGCTCCCCCGCGCTACGCCACG; CTGGAGCACCCCTTCCACTGCCCGCGCGTCCTCAAGGTGCCATCCTATCTCAGCTACAAG; TTTCTGGGCGAGCGTGATTGTGCTGCGCCCTGCGAACCTGCGCGGCCCGATGGTTCCATG; TTCTTCTCACAGGAGGAGACGCGTTTCGCGCGCCTCTGGATCCTCACCTGGTCGGTGCTG; TGCTGCGCTTCCACCTTCTTCACTGTCACCACGTACTTGGTAGACATGCAGCGCTTCCGC; TACCCAGAGCGGCCTATCATTTTTCTGTCGGGCTGCTACACCATGGTGTCGGTGGCCTAC; ATCGCGGGCTTCGTGCTCCAGGAGCGCGTGGTGTGCAACGAGCGCTTCTCCGAGGACGGT; TACCGCACGGTGGTGCAGGGCACCAAGAAGGAGGGCTGCACCATCCTCTTCATGATGCTC; TACTTCTTCAGCATGGCCAGCTCCATCTGGTGGGTCATCCTGTCGCTCACCTGGTTCCTG; GCAGCCGGCATGAAGTGGGGCCACGAGGCCATCGAGGCCAACTCTCAGTACTTCCACCTG; GCCGCCTGGGCCGTGCCGGCCGTCAAGACCATCACCATCCTGGCCATGGGCCAGATCGAC; GGCGACCTGCTGAGCGGCGTGTGCTTCGTAGGCCTCAACAGCCTGGACCCGCTGCGGGGC; TTCGTGCTAGCGCCGCTCTTCGTGTACCTGTTCATCGGCACGTCCTTCCTCCTGGCCGGC; TTCGTGTCGCTCTTCCGCATCCGCACCATCATGAAGCACGACGGCACCAAGACCGAAAAG; CTGGAGCGGCTCATGGTGCGCATCGGCGTCTTCTCCGTGCTCTACACAGTGCCCGCCACC; ATCGTCATCGCTTGCTACTTCTACGAGCAGGCCTTCCGCGAGCACTGGGAGCGCTCGTGG; GTGAGCCAGCACTGCAAGAGCCTGGCCATCCCGTGCCCGGCGCACTACACGCCGCGCATG; TCGCCCGACTTCACGGTCTACATGATCAAATACCTCATGACGCTCATCGTGGGCATCACG; TCGGGCTTCTGGATCTGGTCGGGCAAGACGCTGCACTCGTGGAGGAAGTTCTACACTCGC; CTCACCAACAGCCGACACGGTGAGACCACCGTGTGAGGGACGCCCCCAGGCCGGAACCGC; GCGGCGCTTTCCTCCGCCCGGGGTGGGGCCCCTACAGACTCCGTATTTTATTTTTTTAAA; TAAAAAACGATCGAAACCATTTCACTTTTAGGTTGCTTTTTAAAAGAGAACTCTCTGCCC; AACACCCCCACAAGGTTTGTAATTAAAACTGTAAATAGTCTTTGTAAATTTAATTATATA; TATTTTCTATTTAAAAGAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001466 unedited; GGTCGGATTTTGTATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGGGAGAGA; GGGCAGCAGCGCGCGGTGTCTCCGGCTGCTCAGTCCGACCGCGGCAAGCAAGCGGGCAGG; CGCACCGCCCCCTCCCCCGCCCGGCCTCCCCAACTCTGCGGCCGCGAGTAAAGTTTGCAA; AGAGGCGCGGGAGGCGGCAGCCGCAGCGAGGAGGCGGCGGGGAAGAAGCGCAGTCTCCGG; GTTGGGGGCGGGGGCGGGGGGGGCGCCAAGGAGCCGGGTGGGGGGGCGGNNNNNNNNNTN; TNNNTTNTNNNNNNNNTTTNNTNTTNNTTTTTTTGTNTTTTNTNTTTTTTTTTTTTTTNN; NTTNNNNNNNTTTNTTTNNNNTTGGNNNTTTTNNTTNTTNNTTTTTTTTTTTNTTTTNNN; TTNNTTTTTTTTTTTTTNNTTTTTTTTTTTTTNNTTTTTTNNNTTTTTTTTTTTTTTTTT; TTTTNTTNNTNNTTTNNTTTTTTTTTTTTTTTNTNTTNTTNNTTTTTNNNNNTTNTTTTT; TTTTTTTTTTTTTTTTTTTTNTTTTTTTTTTGGTTTTTTTTTTTTTTTTTTNNNTTTTTC; CTTTTTTTTTNTTTTTTTTTTTNTTTTTTTTTNNTTTTTTTTNTTTTTTTTTTTTTTTTT; TCTTTTTTTTTNTNNTTNTTTTTTTTTTTTTTTNNNNTTNNTTTTNTTTTTTTTTTTNTT; TTTTNTTTTTTTNNTNTTTTTTTTTTTTTTTTTTTTTTTTNTTTNNTTTNNTTTTTTTTT; TTTTTTTTTTTTNNTTTTTTTTTTTTNNNTTTTTTTTNNTTTTTTTTTTTTTTTNTTTTN; TNNTTTNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTTTNTTTTTTTTTTTTTNNT; TNNNTTTTTTTTCCTCCTTTTTTTTTTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001466 unedited; CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTCTTTTAAATAGAAAATATAT; ATAATTAAATTTACAAAGACTATTTACAGTTTTAATTACAAACCTTGTGGGGGTGTTGGG; CAGAGAGTTCTCTTTTAAAAAGCAACCTAAAAGTGAAATGGTTTCGATCGTTTTTTATTT; AAAAAAATAAAATACGGAGTCTGTAGGGGCCCCACCCCGGGCGGAGGAAAGCGCCGCGCG; GTTCCGGCCTGGGGGCGTCCCTCACACGGTGGTCTCACCGTGTCGGCTGTTGGTGAGGCG; AGTGTAAAACTTCCTCCACGAGTGCAGCGTCTTGCCCGACCAGATCCAGAAGCCCGACGT; GATGCCCACGATGAGCGTCATGAGGTATTTGATCATGTAGACCGTGAAGTCGGGCGACAT; GCGCGGCGTGTAGTGCGCCGGGCACGGGATGGCCAGGCTCTTGCAGTGCTGGCTCACCCA; CGAGCGCTTCCAGTGCTCGCGGAAGGCCTGCTCGTAAAAGTAGCAAGCGATGACGATGGT; GGGCGGCACTGTGTAGAGCACGGAGAAGACGCCGATGCGCACCATGAGCCCGCTCAGCTT; TTCGGTCTTGGTGCCGTCGTGCTTCATGATGGGTGCGATGCCGAAGAGCGACACGAAGCC; GCCCAGAGGAAGACGTGCCCATGACAAGTACACGAAGAAGCGCGCTAGCACGAACCCCGC; AGGGGTCCAGCCTGTTGAGCCTACGAGCACACGCCGTTCACAAGTCGCCGTCATCTGCCA; TGGCCAGATGGGATTGTCTTGAAGCGGCCGCCCAGGCGGCAGGGGAGTACTGAAGTTGCC; CTCATTGCTCGTGGCCCACTTATGCCGGTTGCGGAACCCGGTGAGGAAGGATGACCCACA; ATGAGCTGCCTGCTGAAAAGTAACCTCTGAAAGGATGGGCCCCCTTTTTTTGGCCCTGCC; CACGGCGGGAACCGTCTGGAAAAGCTGTTGCACCCCCCCTTCGGAACCAAACCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001466
• Insert Size: 2170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001466.2, NP_001457.1
• RefSeq Size: 1983 bp
• RefSeq ORF: 1698 bp
• Locus ID: 2535
• UniProt ID: Q14332
• Cytogenetics: 17q21.31
• Domains: FRI, Frizzled
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Summary: This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010]