INCENP (NM_020238) Human Tagged ORF Clone

SKU RG220448

INCENP (tGFP-tagged) - Human inner centromere protein antigens 135/155kDa (INCENP), transcript variant 2. Note: ORF is codon optimized

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: INCENP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220448 ORF sequence, codon optimized.; Due to the complexity of NM_020238, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCACCACCGCCCCCGGCCCCATACATCTGTTGGAGCTTTGTGACCAGAAGCTGATGGAATTTCTTT; GTAACATGGACAACAAGGATCTCGTGTGGCTGGAGGAAATCCAAGAAGAGGCTGAACGGATGTTTACACG; CGAATTCAGTAAAGAGCCAGAACTGATGCCCAAAACACCCTCCCAGAAGAACCGGCGCAAGAAAAGAAGG; ATTTCATACGTTCAGGATGAGAACCGCGACCCGATTCGAAGACGGCTCTCAAGACGAAAATCCCGGTCAA; GCCAGCTGAGTTCTAGGCGCCTGCGATCCAAAGATTCCGTGGAGAAGTTGGCTACAGTGGTGGGCGAGAA; TGGATCCGTGCTCCGCCGAGTTACAAGGGCCGCTGCCGCTGCGGCAGCAGCCACGATGGCCCTGGCAGCC; CCGAGCTCCCCAACTCCTGAGAGCCCTACAATGCTTACCAAGAAGCCCGAGGATAATCACACCCAGTGTC; AGCTGGTACCCGTGGTGGAAATCGGAATCAGCGAACGCCAAAACGCAGAACAGCACGTCACCCAGCTCAT; GTCCACCGAGCCGCTCCCCCGGACTTTGAGTCCTACCCCAGCTAGTGCCACAGCCCCTACAAGCCAGGGA; ATACCTACAAGCGATGAAGAATCCACGCCGAAAAAGAGTAAAGCTCGCATCCTGGAGTCCATCACCGTGT; CTTCACTGATGGCGACACCCCAGGACCCAAAGGGTCAAGGCGTCGGTACGGGAAGGTCTGCCTCTAAACT; GCGGATCGCGCAGGTGTCCCCCGGGCCTCGGGATTCACCAGCTTTTCCTGACAGCCCCTGGCGCGAGCGC; GTGCTGGCTCCTATCCTGCCGGACAATTTTTCAACACCAACGGGCTCTAGAACAGATTCCCAGAGCGTCA; GACACAGCCCGATAGCCCCTTCCTCCCCTAGCCCCCAAGTGTTGGCTCAGAAGTATTCTCTTGTGGCCAA; ACAAGAGAGTGTGGTTCGAAGAGCCAGCCGACGCCTCGCTAAGAAGACAGCAGAGGAGCCTGCAGCCAGC; GGTCGGATAATATGTCACTCTTACCTCGAAAGACTGCTCAATGTGGAAGTGCCCCAGAAAGTCGGGTCCG; AGCAGAAGGAACCTCCTGAAGAGGCTGAGCCCGTCGCCGCGGCTGAACCCGAAGTTCCAGAAAATAATGG; CAACAACTCTTGGCCACACAACGACACAGAAATTGCCAATTCTACACCCAATCCTAAACCGGCTGCCTCT; AGCCCTGAGACGCCATCCGCTGGGCAGCAGGAGGCCAAGACGGATCAGGCCGACGGTCCTCGCGAACCCC; CTCAGTCAGCCCGCAGGAAACGCAGCTACAAACAGGCAGTGTCCGAGCTGGATGAAGAACAGCATCTCGA; GGATGAGGAGCTCCAGCCTCCAAGGAGCAAAACCCCTAGCTCCCCCTGTCCCGCTTCCAAAGTTGTGCGC; CCTCTGCGCACTTTTCTTCACACCGTGCAGCGGAACCAGATGCTCATGACCCCAACGAGTGCGCCCCGAT; CAGTGATGAAATCCTTTATTAAGAGGAATACCCCTCTCCGAATGGATCCCAAGGAGAAGGAGCGACAGCG; ATTGGAAAACCTCCGACGAAAAGAAGAGGCGGAGCAGCTGCGCAGGCAGAAGGTGGAGGAGGATAAGAGA; AGACGCCTGGAAGAAGTGAAATTGAAACGAGAGGAAAGGCTGAGAAAGGTGCTCCAGGCCCGAGAACGAG; TCGAGCAGATGAAAGAGGAAAAGAAAAAACAGATCGAACAGAAGTTTGCACAGATTGACGAGAAAACTGA; AAAAGCCAAAGAGGAAAGACTGGCAGAGGAGAAGGCAAAGAAGAAGGCAGCCGCTAAGAAAATGGAAGAG; GTCGAGGCTCGGCGCAAACAGGAGGAAGATGCACGGCGCCTTCGCTGGCTTCAACAGGAGGAGGAGGAGA; GGAGGCATCAGGAACTCCTGCAGAAAAAAAAAGAAGAGGAGCAGGAGAGACTGAGAAAGGCCGCCGAAGC; CAAGCGCCTCGCCGAGCAGCGGGAGCAAGAGCGCAGGGAGCAAGAAAGGCGGGAACAGGAACGCCGCGAG; CAGGAACGCAGAGAACAAGAGAGGCGAGAACAGGAGAGAAGAGAACAGGAGAGGCAACTGGCCGAGCAGG; AGAGAAGGAGAGAGCAAGAGCGACTCCAAGCCGAAAGGGAACTGCAGGAGAGGGAGAAAGCACTGAGGCT; CCAAAAGGAACAGCTGCAGCGGGAACTGGAAGAAAAGAAAAAAAAAGAGGAACAACAGCGACTTGCTGAG; CGACAACTGCAGGAAGAGCAGGAGAAGAAGGCCAAAGAAGCCGCCGGAGCGTCTAAAGCCTTGAACGTGA; CCGTCGACGTTCAGTCTCCCGCCTGCACATCCTCACCTATAACCCCCCAGGGACACAAAGCACCCCCTCA; GATTAACCCACATAACTACGGAATGGACCTCAATTCTGACGACAGTACTGATGACGAGGCCCACCCCCGA; AAGCCTATACCCACCTGGGCGCGCGGAACTCCTCTCAGCCAGGCGATCATCCACCAATATTACCAACCTC; CAAACCTGCTGGAACTCTTTGGCACCATTTTGCCCCTGGATCTGGAGGACATCTTTAAGAAAAGCAAGCC; GAGATATCATAAAAGGACTAGCTCTGCCGTCTGGAACTCCCCACCACTGCAGGGCGCTCGCGTGCCCAGT; TCCTTGGCATATTCACTCAAAAAACAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220448 representing NM_020238; Red=Cloning site Green=Tags(s); ; MGTTAPGPIHLLELCDQKLMEFLCNMDNKDLVWLEEIQEEAERMFTREFSKEPELMPKTPSQKNRRKKRR; ISYVQDENRDPIRRRLSRRKSRSSQLSSRRLRSKDSVEKLATVVGENGSVLRRVTRAAAAAAAATMALAA; PSSPTPESPTMLTKKPEDNHTQCQLVPVVEIGISERQNAEQHVTQLMSTEPLPRTLSPTPASATAPTSQG; IPTSDEESTPKKSKARILESITVSSLMATPQDPKGQGVGTGRSASKLRIAQVSPGPRDSPAFPDSPWRER; VLAPILPDNFSTPTGSRTDSQSVRHSPIAPSSPSPQVLAQKYSLVAKQESVVRRASRRLAKKTAEEPAAS; GRIICHSYLERLLNVEVPQKVGSEQKEPPEEAEPVAAAEPEVPENNGNNSWPHNDTEIANSTPNPKPAAS; SPETPSAGQQEAKTDQADGPREPPQSARRKRSYKQAVSELDEEQHLEDEELQPPRSKTPSSPCPASKVVR; PLRTFLHTVQRNQMLMTPTSAPRSVMKSFIKRNTPLRMDPKEKERQRLENLRRKEEAEQLRRQKVEEDKR; RRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEE; VEARRKQEEDARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERRE; QERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAE; RQLQEEQEKKAKEAAGASKALNVTVDVQSPACTSSPITPQGHKAPPQINPHNYGMDLNSDDSTDDEAHPR; KPIPTWARGTPLSQAIIHQYYQPPNLLELFGTILPLDLEDIFKKSKPRYHKRTSSAVWNSPPLQGARVPS; SLAYSLKKH; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020238
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020238.1, NP_064623.1
• RefSeq Size: 4134 bp
• RefSeq ORF: 2745 bp
• Locus ID: 3619
• UniProt ID: Q9NQS7
• Cytogenetics: 11q12.3
• Protein Families: Druggable Genome
• Summary: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]