INCENP (NM_020238) Human Untagged Clone

SKU SC304727

INCENP (untagged)-Human inner centromere protein antigens 135/155kDa (INCENP), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: INCENP
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020238, the custom clone sequence may differ by one or more nucleotides; ATGGGGACGACGGCCCCAGGGCCCATTCACCTGCTGGAGCTATGTGACCAGAAGCTCATG; GAGTTTCTCTGCAACATGGATAATAAGGACTTGGTGTGGCTTGAGGAAATCCAAGAGGAG; GCCGAGCGCATGTTCACCAGAGAATTCAGCAAAGAGCCAGAGCTGATGCCCAAAACACCT; TCTCAGAAGAACCGACGGAAGAAGAGACGGATTTCTTATGTTCAGGATGAAAACAGAGAT; CCCATCAGGAGAAGGTTATCCCGCAGAAAGTCTCGGAGCAGCCAGCTGAGCTCCCGACGC; CTCCGCAGCAAGGACAGTGTAGAGAAGCTGGCTACAGTGGTCGGGGAGAACGGCTCCGTC; CTGCGGCGTGTGACCCGTGCTGCGGCTGCAGCTGCCGCGGCTACCATGGCATTGGCTGCA; CCTTCTTCACCCACCCCTGAGTCTCCCACGATGCTGACTAAGAAGCCCGAGGATAACCAC; ACCCAGTGCCAGCTGGTGCCTGTGGTGGAGATCGGCATCAGTGAGCGCCAGAATGCTGAG; CAGCATGTCACCCAGCTCATGTCCACCGAGCCTCTGCCCCGCACTCTGTCCCCGACTCCA; GCTTCAGCCACAGCTCCAACCTCCCAGGGCATCCCGACATCAGATGAGGAATCAACACCT; AAGAAGTCGAAGGCCAGGATACTGGAGTCCATCACAGTGAGCTCCCTGATGGCTACACCC; CAGGACCCCAAGGGTCAAGGGGTCGGGACGGGGCGGTCTGCGTCTAAGCTCAGGATTGCG; CAGGTCTCCCCTGGCCCACGGGACTCGCCAGCCTTTCCAGATTCTCCATGGCGGGAGCGG; GTGCTGGCTCCCATCCTGCCGGATAACTTCTCCACGCCCACGGGCTCTCGCACGGACTCT; CAATCGGTGCGGCACAGCCCGATCGCCCCGTCTTCCCCGAGTCCCCAAGTCTTAGCCCAG; AAGTACTCTCTGGTGGCCAAACAGGAAAGTGTTGTCCGCAGGGCGAGCAGAAGGCTTGCC; AAGAAGACTGCCGAAGAGCCAGCTGCCTCTGGCCGCATCATCTGTCACAGTTACCTGGAG; AGGCTCCTGAATGTTGAGGTGCCCCAGAAAGTTGGTTCTGAGCAGAAGGAACCCCCCGAG; GAGGCTGAGCCTGTGGCGGCAGCTGAGCCAGAGGTCCCTGAGAACAATGGAAATAACTCG; TGGCCCCACAATGACACGGAGATTGCCAACAGCACACCCAACCCGAAGCCTGCAGCCAGC; AGCCCGGAAACACCCTCTGCAGGGCAGCAAGAGGCCAAGACGGACCAAGCAGATGGACCC; AGAGAGCCACCGCAGAGTGCCAGGAGGAAGCGCAGCTACAAGCAGGCCGTGAGTGAGCTG; GACGAGGAGCAGCACCTGGAGGATGAGGAGCTGCAGCCCCCCAGGAGCAAGACCCCTTCC; TCACCCTGCCCAGCCAGCAAGGTGGTACGGCCCCTCCGGACCTTTCTGCACACAGTGCAG; AGGAACCAGATGCTCATGACCCCGACCTCAGCCCCACGCAGCGTCATGAAGTCCTTTATT; AAGCGCAACACTCCCCTGCGCATGGACCCCAAGGAGAAGGAGCGGCAGCGCCTGGAGAAT; CTGCGGCGGAAGGAGGAGGCCGAGCAGCTGCGCAGGCAGAAGGTGGAGGAGGACAAGCGG; CGGCGGCTGGAGGAGGTGAAGCTGAAGCGTGAGGAACGCCTCCGCAAGGTGCTGCAGGCC; CGCGAGCGGGTGGAGCAGATGAAGGAGGAGAAGAAGAAGCAGATTGAGCAGAAGTTTGCT; CAGATCGACGAGAAGACTGAGAAGGCCAAGGAGGAGCGGCTGGCAGAGGAGAAGGCCAAG; AAAAAGGCGGCGGCCAAGAAGATGGAGGAGGTGGAAGCACGCAGGAAGCAGGAAGAGGAG; GCACGTAGGCTCAGGTGGCTGCAGCAGGAGGAGGAAGAGCGGCGGCACCAAGAGCTGCTG; CAGAAGAAGAAGGAAGAGGAGCAGGAGCGGCTGCGGAAGGCGGCCGAGGCTAAGCGGCTG; GCAGAGCAGCGGGAGCAGGAGCGGCGGGAGCAGGAGCGGCGCGAGCAGGAGCGGCGCGAG; CAGGAGCGGCGGGAGCAGGAGCGGCGCGAGCAGGAGCGACAGCTGGCAGAGCAGGAGCGT; CGGCGGGAGCAGGAGCGGCTCCAGGCCGAGAGGGAGCTGCAGGAGCGGGAGAAGGCCCTG; CGGCTGCAGAAGGAGCAGCTGCAGAGGGAACTGGAGGAGAAGAAGAAGAAGGAAGAGCAG; CAGCGTCTGGCTGAGCGGCAGCTGCAGGAGGAGCAAGAGAAGAAAGCCAAGGAGGCAGCA; GGGGCCAGCAAGGCCCTGAATGTGACTGTGGACGTGCAGTCTCCAGCTTGTACCTCATAT; CAGATGACTCCGCAAGGGCACAGGGCCCCTCCCAAGATCAACCCAGATAACTACGGGATG; GATCTGAATAGCGACGACTCCACCGATGATGAGGCCCATCCCCGGAAGCCCATCCCCACC; TGGGCCCGAGGCACCCCGCTCAGCCAGGCTATCATTCACCAGTACTACCACCCACCGAAC; CTTCTGGAGCTCTTTGGAACCATTCTCCCACTGGACTTGGAGGATATCTTCAAGAAGAGC; AAGCCCCGCTATCACAAGCGCACCAGCTCTGCTGTCTGGAACTCACCGCCCCTGCAGGGC; GCCAGGGTCCCCAGCAGCCTGGCCTACAGCCTGAAGAAGCACTGA;
• Restriction Sites: Please inquire
• ACCN: NM_020238
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020238.1, NP_064623.1
• RefSeq Size: 3536 bp
• RefSeq ORF: 2760 bp
• Locus ID: 3619
• UniProt ID: Q9NQS7
• Cytogenetics: 11q12.3
• Protein Families: Druggable Genome
• Summary: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.