CLACP (COL25A1) (NM_198721) Human Tagged ORF Clone

SKU RG217472

COL25A1 (tGFP-tagged) - Human collagen, type XXV, alpha 1 (COL25A1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CLACP
• Synonyms: AMY; CFEOM5; CLAC; CLAC-P; CLACP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217472 representing NM_198721; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTGCTGAAGAAGCACGCAGGGAAAGGAGGGGGCCGGGAGCCCAGATCCGAGGACCCGACCCCTGCCG; AACAGCATTGTGCCCGGACCATGCCCCCGTGTGCCGTCCTGGCGGCCCTCCTGTCAGTGGTGGCCGTGGT; GTCTTGCCTGTACCTGGGTGTGAAAACCAACGACCTCCAGGCGAGGATCGCCGCTCTCGAATCCGCCAAA; GGGGCCCCTTCCATTCATCTGCTGCCTGATACCCTGGATCACCTCAAGACTATGGTGCAAGAGAAAGTGG; AGCGACTTCTGGCTCAGAAATCCTATGAACATATGGCTAAAATAAGAATCGCAAGAGAAGCACCTTCAGA; ATGTAACTGCCCAGCAGGCCCTCCAGGGAAACGAGGTAAGAGAGGCCGAAGAGGAGAATCTGGTCCTCCT; GGACAGCCTGGTCCTCAGGGCCCTCCTGGTCCAAAAGGCGATAAGGGAGAACAAGGTGATCAGGGACCTA; GGATGGTGTTTCCTAAAATCAATCATGGGTTTCTCTCTGCTGATCAGCAGCTCATTAAACGCCGCCTGAT; TAAGGGTGACCAAGGACAGGCAGGGCCTCCAGGACCCCCTGGCCCTCCAGGCCCAAGAGGGCCACCTGGG; GACACAGGGAAAGATGGCCCCCGTGGAATGCCAGGAGTACCCGGTGAACCAGGAAAGCCAGGAGAACAAG; GCTTGATGGGTCCTCTAGGGCCTCCGGGACAAAAGGGTTCTATTGGAGCACCTGGAATTCCAGGGATGAA; TGGGCAAAAGGGTGAGCCCGGGTTGCCTGGAGCAGTAGGACAGAATGGAATACCAGGACCTAAGGGAGAA; CCTGGAGAACAAGGTGAAAAGGGAGACGCTGGAGAGAACGGCCCCAAGGGTGACACAGGCGAAAAGGGTG; ACCCTGGATCATCTGCTGCAGGAATTAAGGGAGAACCTGGGGAATCTGGTCGTCCAGGGCAAAAGGGTGA; ACCAGGGCTTCCTGGGCTTCCTGGACTTCCGGGGATAAAGGGAGAACCAGGTTTCATTGGTCCTCAAGGA; GAACCAGGCTTACCAGGTTTACCAGGAACAAAAGGTGAACGGGGGGAAGCAGGGCCTCCTGGAAGAGGTG; AGCGAGGGGAACCTGGAGCCCCCGGACCAAAGGGGAAACAAGGTGAATCAGGAACTAGAGGCCCAAAGGG; GTCAAAGGGGGATCGTGGAGAAAAAGGGGACTCTGGAGCTCAGGGACCAAGGGGTCCACCTGGTCAAAAA; GGGGATCAAGGAGCCACTGAGATCATAGACTACAACGGCAACCTCCACGAAGCCTTACAGAGGATTACCA; CCTTAACTGTCACGGGTCCCCCTGGACCTCCTGGACCTCAAGGACTACAAGGGCCAAAGGGAGAGCAGGG; ATCTCCAGGAATCCCAGGAATGGATGGAGAGCAGGGACTCAAAGGCTCAAAGGGAGACATGGGGGACCCA; GGTATGACAGGTGAAAAAGGAGGAATTGGACTTCCTGGATTACCGGGAGCCAATGGAATGAAAGGAGAAA; AAGGAGATTCTGGAATGCCGGGTCCACAGGGTCCTTCTATCATAGGCCCACCAGGCCCACCAGGTCCCCA; TGGCCCACCTGGCCCCATGGGACCTCATGGACTTCCTGGACCAAAGGGTACAGATGGTCCTATGGGACCC; CATGGCCCTGCAGGTCCCAAAGGAGAAAGAGGTGAAAAAGGAGCTATGGGAGAGCCTGGACCAAGAGGGC; CCTATGGGCTGCCTGGGAAAGATGGAGAGCCTGGTCTTGATGGCTTCCCTGGTCCACGGGGTGAGAAGGG; TGATCTAGGAGAAAAGGGAGAAAAGGGATTCCGTGGCGTTAAGGGGGAAAAAGGGGAGCCAGGCCAGCCT; GGCCTGGATGGGCTGGATGCCCCTTGCCAATTGGGGCCAGATGGCTTACCCATGCCTGGCTGTTGGCAAA; AG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217472 representing NM_198721; Red=Cloning site Green=Tags(s); ; MLLKKHAGKGGGREPRSEDPTPAEQHCARTMPPCAVLAALLSVVAVVSCLYLGVKTNDLQARIAALESAK; GAPSIHLLPDTLDHLKTMVQEKVERLLAQKSYEHMAKIRIAREAPSECNCPAGPPGKRGKRGRRGESGPP; GQPGPQGPPGPKGDKGEQGDQGPRMVFPKINHGFLSADQQLIKRRLIKGDQGQAGPPGPPGPPGPRGPPG; DTGKDGPRGMPGVPGEPGKPGEQGLMGPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGE; PGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQG; EPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQK; GDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGPKGEQGSPGIPGMDGEQGLKGSKGDMGDP; GMTGEKGGIGLPGLPGANGMKGEKGDSGMPGPQGPSIIGPPGPPGPHGPPGPMGPHGLPGPKGTDGPMGP; HGPAGPKGERGEKGAMGEPGPRGPYGLPGKDGEPGLDGFPGPRGEKGDLGEKGEKGFRGVKGEKGEPGQP; GLDGLDAPCQLGPDGLPMPGCWQK; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198721
• ORF Size: 1962 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198721.4
• RefSeq Size: 2607 bp
• RefSeq ORF: 1965 bp
• Locus ID: 84570
• UniProt ID: Q9BXS0
• Cytogenetics: 4q25
• Protein Families: Transmembrane
• Summary: This gene encodes a brain-specific membrane associated collagen. A product of proteolytic processing of the encoded protein, CLAC (collagenous Alzheimer amyloid plaque component), binds to amyloid beta-peptides found in Alzheimer amyloid plaques but CLAC inhibits rather than facilitates amyloid fibril elongation (PMID: 16300410). A study of over-expression of this collagen in mice, however, found changes in pathology and behavior suggesting that the encoded protein may promote amyloid plaque formation (PMID: 19548013). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]