RECQL5 (NM_001003716) Human Tagged ORF Clone
SKU
RG216761
RECQL5 (tGFP-tagged) - Human RecQ protein-like 5 (RECQL5), transcript variant 3
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TrueORF®
TrueORF®
Expression-ready ORF plasmid with C-terminal tag(s)
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Product Data | |
Type | Human Tagged ORF Clone |
---|---|
Target Symbol | RECQL5 |
Synonyms | RECQ5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RG216761 representing NM_001003716
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCCACCATACCACCTTTCCTTTTGACCCTGAGCGGCGAGTCCGGAGTACGCTGAAGAAGGTCT TTGGGTTTGACTCTTTTAAGACGCCTTTACAGGAGAGTGCGACCATGGCTGTAGTAAAAGGTAACAAGGA CGTCTTTGTGTGCATGCCCACAGGGGCAGGAAAATCCCTATGCTATCAGCTCCCTGCTCTGTTGGCCAAA GGCATCACCATTGTAGTCTCTCCTCTCATTGCTTTGATTCAGGACCAAGTGGACCACTTGCTAACCCTAA AGGTACGAGTAAGTTCCCTGAACTCGAAGCTCTCTGCACAGGAAAGGAAGGAGCTGCTTGCTGACCTGGA GCGAGAAAAGCCCCAGACCAAGATTCTGTACATCACCCCAGAGATGGCAGCTTCATCCTCCTTCCAGCCC ACCCTGAACTCCCTGGTGTCCCGCCACCTGCTGTCTTACTTGGTGGTGGATGAAGCTCATTGTGTTTCCC AATGGGGGCATGACTTTCGTCCTGACTACTTGCGTCTGGGTGCCCTGCGCTCCCGCCTGGGACATGCCCC TTGTGTGGCTCTGACCGCCACAGCCACCCCACAGGTCCAAGAGGACGTGTTTGCTGCCCTGCACCTGAAG AAACCAGTTGCCATCTTCAAGACTCCCTGCTTCCGGGCCAACCTCTTCTATGATGTGCAATTCAAGGAAC TGATTTCTGATCCCTATGGGAACCTGAAGGACTTCTGCCTTAAGGCTCTTGGACAGGAGGCTGATAAAGG GTTATCTGGCTGCGGCATTGTGTACTGCAGGACTAGAGAGGCTTGTGAACAGCTGGCCATAGAGCTCAGC TGCAGGGGTGTGAACGCCAAGGCTTACCATGCAGGGCTGAAGGCCTCTGAAAGAACGCTGGTGCAGAACG ACTGGATGGAGGAGAAGGTCCCTGTAATTGTTGCAACCATTAGTTTTGGGATGGGAGTGGATAAAGCCAA TGTCAGGTTTGTCGCCCATTGGAATATTGCCAAGTCTATGGCTGGGTACTACCAGGAGTCTGGCCGGGCT GGCAGGGATGGGAAGCCTTCCTGGTGCCGTCTCTATTACTCCAGGAATGACCGGGACCAAGTCAGCTTCC TGATCAGGAAGGAAGTAGCAAAACTCCAGGAAAAGAGAGGAAACAAAGCATCTGATAAAGCCACTATCAT GGCCTTTGATGCCCTGGTGACCTTCTGTGAAGAACTGGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG216761 representing NM_001003716
Red=Cloning site Green=Tags(s) MSSHHTTFPFDPERRVRSTLKKVFGFDSFKTPLQESATMAVVKGNKDVFVCMPTGAGKSLCYQLPALLAK GITIVVSPLIALIQDQVDHLLTLKVRVSSLNSKLSAQERKELLADLEREKPQTKILYITPEMAASSSFQP TLNSLVSRHLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLGHAPCVALTATATPQVQEDVFAALHLK KPVAIFKTPCFRANLFYDVQFKELISDPYGNLKDFCLKALGQEADKGLSGCGIVYCRTREACEQLAIELS CRGVNAKAYHAGLKASERTLVQNDWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGRA GRDGKPSWCRLYYSRNDRDQVSFLIRKEVAKLQEKRGNKASDKATIMAFDALVTFCEELG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001003716 |
ORF Size | 1230 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_001003716.4 |
RefSeq Size | 3710 bp |
RefSeq ORF | 1233 bp |
Locus ID | 9400 |
UniProt ID | O94762 |
Cytogenetics | 17q25.1 |
Summary | The protein encoded by this gene is a helicase that is important for genome stability. The encoded protein also prevents aberrant homologous recombination by displacing RAD51 from ssDNA. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC216761 | RECQL5 (Myc-DDK-tagged)-Human RecQ protein-like 5 (RECQL5), transcript variant 3 | 10 ug |
$457.00
|
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RC216761L3 | Lenti ORF clone of Human RecQ protein-like 5 (RECQL5), transcript variant 3, Myc-DDK-tagged | 10 ug |
$757.00
|
|
RC216761L4 | Lenti ORF clone of Human RecQ protein-like 5 (RECQL5), transcript variant 3, mGFP tagged | 10 ug |
$757.00
|
|
SC300533 | RECQL5 (untagged)-Human RecQ protein-like 5 (RECQL5), transcript variant 3 | 10 ug |
$503.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.