SPPL3 (NM_139015) Human Tagged ORF Clone

SKU RG210096

SPPL3 (tGFP-tagged) - Human signal peptide peptidase 3 (SPPL3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SPPL3
• Synonyms: IMP2; MDHV1887; PRO4332; PSH1; PSL4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210096 representing NM_139015; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGAGCAGACCTACTCGTGGGCCTATTCCCTGGTGGATTCCAGTCAAGTGTCTACATTTCTGATTT; CCATTCTTCTTATAGTCTATGGTAGTTTCAGGTCCCTTAATATGGACTTTGAAAATCAAGATAAGGAGAA; AGACAGTAATAGTTCTTCTGGGTCTTTCAATGGCAACAGCACCAATAATAGCATCCAAACAATTGACTCT; ACCCAGGCTCTGTTCCTTCCAATTGGAGCATCTGTCTCTCTTTTAGTAATGTTCTTCTTCTTTGACTCAG; TTCAAGTAGTTTTTACAATATGTACAGCAGTTCTTGCAACGATAGCTTTTGCTTTTCTTCTCCTCCCGAT; GTGCCAGTATTTAACAAGACCCTGCTCACCTCAGAACAAGATTTCCTTTGGTTGCTGTGGACGTTTCACT; GCTGCTGAGTTGCTGTCATTCTCTCTGTCTGTCATGCTCGTCCTCATCTGGGTTCTCACTGGCCATTGGC; TTCTCATGGATGCACTGGCCATGGGCCTCTGTGTCGCCATGATCGCCTTTGTCCGCCTGCCGAGCCTCAA; GGTCTCCTGCCTGCTTCTCTCAGGGCTTCTCATCTATGATGTCTTTTGGGTATTTTTCTCAGCCTACATC; TTCAATAGCAACGTCATGGTGAAGGTGGCCACTCAGCCGGCTGACAATCCCCTTGACGTTCTATCCCGGA; AGCTCCACCTGGGGCCCAATGTTGGGCGTGATGTTCCTCGCCTGTCTCTGCCTGGAAAACTGGTCTTCCC; AAGCTCCACTGGCAGCCACTTCTCCATGTTGGGCATCGGAGACATCGTTATGCCTGGTCTCCTACTATGC; TTTGTCCTTCGCTATGACAACTACAAAAAGCAAGCCAGTGGGGACTCCTGTGGGGCCCCTGGACCTGCCA; ACATCTCCGGGCGCATGCAGAAGGTCTCCTACTTTCACTGCACCCTCATCGGATACTTTGTAGGCCTGCT; CACTGCTACTGTGGCGTCTCGCATTCACCGGGCCGCCCAGCCCGCCCTTCTCTATTTGGTGCCATTTACT; TTATTGCCACTCCTCACGATGGCCTATTTAAAGGGCGACCTCCGGCGGATGTGGTCTGAGCCTTTCCACT; CCAAGTCCAGCAGCTCCCGATTCCTGGAAGTA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210096 representing NM_139015; Red=Cloning site Green=Tags(s); ; MAEQTYSWAYSLVDSSQVSTFLISILLIVYGSFRSLNMDFENQDKEKDSNSSSGSFNGNSTNNSIQTIDS; TQALFLPIGASVSLLVMFFFFDSVQVVFTICTAVLATIAFAFLLLPMCQYLTRPCSPQNKISFGCCGRFT; AAELLSFSLSVMLVLIWVLTGHWLLMDALAMGLCVAMIAFVRLPSLKVSCLLLSGLLIYDVFWVFFSAYI; FNSNVMVKVATQPADNPLDVLSRKLHLGPNVGRDVPRLSLPGKLVFPSSTGSHFSMLGIGDIVMPGLLLC; FVLRYDNYKKQASGDSCGAPGPANISGRMQKVSYFHCTLIGYFVGLLTATVASRIHRAAQPALLYLVPFT; LLPLLTMAYLKGDLRRMWSEPFHSKSSSSRFLEV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139015
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_139015.5
• RefSeq Size: 3432 bp
• RefSeq ORF: 1155 bp
• Locus ID: 121665
• UniProt ID: Q8TCT6
• Cytogenetics: 12q24.31
• Domains: DUF435, PSN
• Protein Families: Protease, Transmembrane
• Summary: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries (PubMed:16873890, PubMed:25354954, PubMed:25827571). Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Catalyzes the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis (PubMed:23132852). May also have the ability to serve as a shedding protease for subsequent intramembrane proteolysis by SPPL2A and SPPL2B of the envelope glycoprotein gp130 (PubMed:23132852). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971).[UniProtKB/Swiss-Prot Function]