JIP1 (MAPK8IP1) (NM_005456) Human Tagged ORF Clone

SKU RG209734

MAPK8IP1 (tGFP-tagged) - Human mitogen-activated protein kinase 8 interacting protein 1 (MAPK8IP1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: JIP1
• Synonyms: IB1; JIP-1; JIP1; PRKM8IP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209734 representing NM_005456; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGAGCGAGAAAGCGGCGGCCTGGGAGGGGGGGCCGCGTCCCCGCCCGCCGCCTCCCCGTTCCTGG; GGCTGCACATCGCTTCGCCTCCCAATTTCAGGCTCACCCATGACATCAGCCTGGAGGAGTTTGAGGATGA; AGACCTCTCGGAGATCACTGATGAGTGTGGCATCAGCTTACAGTGCAAAGACACCCTGTCCTTACGGCCC; CCGCGCGCCGGGCTGCTCTCTGCGGGCGGCGGCGGCGCGGGGAGCCGGTTGCAGGCCGAGATGCTGCAGA; TGGACCTGATCGACGCGACGGGGGACACTCCCGGGGCCGAGGACGACGAGGAGGACGACGACGAGGAGCG; CGCGGCCCGGCGGCCGGGAGCGGGGCCGCCCAAGGCCGAGTCCGGCCAGGAGCCGGCGTCCCGCGGCCAG; GGCCAGAGCCAAGGCCAGAGCCAGGGCCCGGGCAGCGGGGACACGTACCGGCCCAAGCGGCCCACCACGC; TCAACCTCTTTCCGCAGGTGCCGCGGTCTCAGGACACACTGAATAATAATTCTCTGGGCAAAAAGCACAG; TTGGCAGGATCGGGTGTCTCGATCATCCTCACCCCTCAAGACAGGGGAGCAGACACCACCGCATGAACAC; ATCTGCGTGAGCGATGAGCTGTCCCCCCAGAGCGGCCCCGCCCCCACCACAGGTCGAGGCACCTCCACCG; ACAGCCCTTGCCGCCGCAGCACAGCCACCCAGATGGCACCTCCGGGTGGTCCCCCTGCTGCCACGCCTGG; GGGTCGGGGCCACTCGCATCGAGACCGAATCCACTACCAGGCCGATGTGCGACTAGAGGCCACTGAGGAG; ATCTACCTGACCCCAGTGCAGAGGCCCCCAGACGCTGCAGAGCCCACCTCCGCCTTCCTGCCGCCCACTG; AGAGCCGGATGTCAGTCAGCTCCGATCCAGACCCTGCCGCCTACCCCTCCACGGCAGGGCGGCCGCACCC; CTCCATCAGTGAAGAGGAAGAGGGCTTCGACTGCATGTCGTCCCCAGAGCGGGCTGAGCCCCCAGGCGGA; GGGTGGCGGGGGAGCCTGGGGGAGCCGCCGCCACCTCCACGGGCCTCTCTGAGCTCGGACACCAGCGCCC; TGTCCTATGACTCTGTCAAGTACACGCTGGTGGTAGATGAGCATGCACAGCTGGAGCTGGTGAGCCTGCG; GCCGTGCTTCGGAGACTACAGTGACGAGAGTGACTCTGCCACCGTCTATGACAACTGTGCCTCCGTCTCC; TCGCCCTATGAGTCGGCCATCGGAGAGGAATATGAGGAGGCCCCGCGGCCCCAGCCCCCTGCCTGCCTCT; CCGAGGACTCCACGCCTGATGAACCCGACGTCCATTTCTCCAAGAAATTCCTGAACGTCTTCATGAGTGG; CCGCTCCCGCTCCTCCAGTGCTGAGTCCTTCGGGCTGTTCTCCTGCATCATCAACGGGGAGGAGCAGGAG; CAGACCCACCGGGCCATATTCAGGTTTGTGCCTCGACACGAAGACGAACTTGAGCTGGAAGTGGATGACC; CTCTGCTAGTGGAGCTCCAGGCTGAAGACTACTGGTACGAGGCCTACAACATGCGCACTGGTGCCCGGGG; TGTCTTTCCTGCCTATTACGCCATCGAGGTCACCAAGGAGCCCGAGCACATGGCAGCCCTGGCCAAAAAC; AGTGACTGGGTGGACCAGTTCCGGGTGAAGTTCCTGGGCTCAGTCCAGGTTCCCTATCACAAGGGCAATG; ACGTCCTCTGTGCTGCTATGCAAAAGATTGCCACCACCCGCCGGCTCACCGTGCACTTTAACCCGCCCTC; CAGCTGTGTCCTGGAGATCAACGTGCGGGGTGTGAAGATAGGCGTCAAGGCCGATGACTCCCAGGAGGCC; AAGGGGAATAAATGTAGCCACTTTTTCCAGTTAAAAAACATCTCTTTCTGCGGATATCATCCAAAGAACA; ACAAGTACTTTGGGTTCATCACCAAGCACCCCGCCGACAACCGGTTTGCCTGCCACGTCTTTGTGTCTGA; AGACTCCACCAAAGCCCTGGCAGAGTCCGTGGGGAGAGCATTCCAGCAGTTCTACAAGCAGTTTGTGGAG; TACACCTGCCCCACAGAAGATATCTACCTGGAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209734 representing NM_005456; Red=Cloning site Green=Tags(s); ; MAERESGGLGGGAASPPAASPFLGLHIASPPNFRLTHDISLEEFEDEDLSEITDECGISLQCKDTLSLRP; PRAGLLSAGGGGAGSRLQAEMLQMDLIDATGDTPGAEDDEEDDDEERAARRPGAGPPKAESGQEPASRGQ; GQSQGQSQGPGSGDTYRPKRPTTLNLFPQVPRSQDTLNNNSLGKKHSWQDRVSRSSSPLKTGEQTPPHEH; ICVSDELSPQSGPAPTTGRGTSTDSPCRRSTATQMAPPGGPPAATPGGRGHSHRDRIHYQADVRLEATEE; IYLTPVQRPPDAAEPTSAFLPPTESRMSVSSDPDPAAYPSTAGRPHPSISEEEEGFDCMSSPERAEPPGG; GWRGSLGEPPPPPRASLSSDTSALSYDSVKYTLVVDEHAQLELVSLRPCFGDYSDESDSATVYDNCASVS; SPYESAIGEEYEEAPRPQPPACLSEDSTPDEPDVHFSKKFLNVFMSGRSRSSSAESFGLFSCIINGEEQE; QTHRAIFRFVPRHEDELELEVDDPLLVELQAEDYWYEAYNMRTGARGVFPAYYAIEVTKEPEHMAALAKN; SDWVDQFRVKFLGSVQVPYHKGNDVLCAAMQKIATTRRLTVHFNPPSSCVLEINVRGVKIGVKADDSQEA; KGNKCSHFFQLKNISFCGYHPKNNKYFGFITKHPADNRFACHVFVSEDSTKALAESVGRAFQQFYKQFVE; YTCPTEDIYLE; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005456
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005456.2, NP_005447.1
• RefSeq Size: 3079 bp
• RefSeq ORF: 2136 bp
• Locus ID: 9479
• UniProt ID: Q9UQF2
• Cytogenetics: 11p11.2
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway
• Summary: This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011]