PPP1R15B (NM_032833) Human Tagged ORF Clone

SKU RG209489

PPP1R15B (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 15B (PPP1R15B)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PPP1R15B
• Synonyms: CREP; MSSGM2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209489 representing NM_032833; Red=Cloning site Blue=ORF Green=Tags(s); ; GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGGCGCGCCC; ; ATGGAGCCGGGGACAGGCGGATCGCGGAAACGGCTTGGCCCTCGGGCGGGCTTCCGGTTCTGGCCACCCT; TTTTCCCTCGGCGATCGCAAGCAGGCTCTTCTAAGTTCCCGACGCCTCTTGGCCCGGAAAACTCCGGGAA; CCCCACACTGCTTTCCTCTGCCCAGCCCGAGACTCGGGTCAGTTACTGGACGAAACTGCTCTCCCAGCTC; CTTGCGCCGCTCCCCGGATTGCTTCAGAAGGTGCTAATTTGGAGCCAACTTTTCGGTGGAATGTTTCCGA; CCAGATGGCTAGATTTTGCTGGAGTCTACAGCGCCCTGAGAGCCCTGAAGGGACGGGAGAAACCAGCCGC; CCCCACAGCGCAGAAATCTTTGAGTTCGCTGCAGCTCGACTCCTCAGACCCCTCGGTCACCAGTCCCCTT; GATTGGCTAGAGGAGGGGATCCACTGGCAATACTCGCCCCCAGACCTAAAATTGGAGCTTAAGGCCAAGG; GAAGTGCTTTGGACCCTGCAGCACAGGCTTTTCTCTTAGAGCAGCAGCTGTGGGGAGTGGAGCTGTTGCC; CAGTAGCCTTCAATCCCGTCTGTACTCTAACCGGGAACTTGGCTCTTCGCCCTCTGGGCCTCTAAACATT; CAACGCATAGACAATTTCAGTGTGGTATCCTATTTGCTGAACCCTTCCTACCTGGACTGCTTTCCTAGGC; TAGAAGTCAGCTATCAGAACAGTGATGGAAATAGCGAGGTAGTCGGCTTCCAGACACTAACCCCAGAGAG; CAGCTGCCTGAGAGAGGACCATTGTCATCCCCAGCCGCTGAGTGCAGAACTCATTCCGGCCTCGTGGCAG; GGATGTCCACCTCTTTCTACGGAAGGCCTACCAGAAATTCACCATCTTCGCATGAAACGGCTGGAATTCC; TTCAACAGGCTAGCAAGGGGCAAGATTTACCCACCCCTGACCAGGATAATGGCTACCACAGCCTGGAGGA; GGAACACAGCCTTCTCCGGATGGATCCAAAACACTGCAGAGATAACCCAACACAGTTTGTTCCTGCTGCT; GGAGACATTCCTGGAAACACCCAGGAATCCACTGAAGAAAAAATAGAATTATTAACTACAGAGGTTCCAC; TTGCTTTGGAAGAAGAGAGCCCTTCTGAGGGCTGTCCATCTAGTGAGATACCTATGGAAAAGGAGCCTGG; AGAGGGCCGAATAAGTGTAGTTGATTACTCATACCTAGAAGGTGACCTTCCCATTTCTGCCAGACCAGCT; TGTAGTAACAAACTGATAGATTATATTTTGGGAGGTGCATCCAGTGACCTGGAAACAAGTTCTGATCCAG; AAGGTGAGGATTGGGATGAGGAAGCTGAGGATGATGGTTTTGATAGTGATAGCTCACTGTCAGACTCAGA; CCTTGAACAAGACCCTGAAGGGCTTCACCTTTGGAACTCTTTCTGCAGTGTAGATCCTTATAATCCCCAG; AACTTTACAGCAACAATTCAGACTGCTGCCAGAATTGTTCCTGAAGAGCCTTCTGATTCAGAGAAGGATT; TGTCTGGCAAGTCTGATCTAGAGAATTCCTCCCAGTCTGGAAGCCTTCCTGAGACCCCTGAGCATAGTTC; TGGGGAGGAAGATGACTGGGAATCTAGTGCAGATGAAGCAGAGAGTCTCAAACTGTGGAACTCATTCTGT; AATTCTGATGACCCCTACAACCCTTTAAATTTTAAGGCTCCTTTTCAAACATCAGGGGAAAATGAGAAAG; GCTGTCGTGACTCAGAGACCCCATCTGAGTCCATTGTGGCCATTTCTGAGTGTCACACCTTACTTTCTTG; TAAGGTGCAGCTGTTGGGGAGCCAAGAAAGTGAATGTCCAGACTCGGTACAGCGTGACGTTCTTTCTGGA; GGAAGACACACACATGTCAAAAGAAAAAAGGTAACCTTCCTTGAAGAAGTTACTGAGTATTATATAAGTG; GTGATGAGGATCGCAAAGGACCATGGGAAGAATTTGCAAGGGATGGATGCAGGTTCCAGAAACGAATTCA; AGAAACAGAAGATGCTATTGGATATTGCTTGACATTTGAACACAGAGAAAGAATGTTTAATAGACTCCAG; GGAACATGCTTCAAAGGACTTAATGTTCTCAAGCAATGT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209489 representing NM_032833; Red=Cloning site Green=Tags(s); ; MEPGTGGSRKRLGPRAGFRFWPPFFPRRSQAGSSKFPTPLGPENSGNPTLLSSAQPETRVSYWTKLLSQL; LAPLPGLLQKVLIWSQLFGGMFPTRWLDFAGVYSALRALKGREKPAAPTAQKSLSSLQLDSSDPSVTSPL; DWLEEGIHWQYSPPDLKLELKAKGSALDPAAQAFLLEQQLWGVELLPSSLQSRLYSNRELGSSPSGPLNI; QRIDNFSVVSYLLNPSYLDCFPRLEVSYQNSDGNSEVVGFQTLTPESSCLREDHCHPQPLSAELIPASWQ; GCPPLSTEGLPEIHHLRMKRLEFLQQASKGQDLPTPDQDNGYHSLEEEHSLLRMDPKHCRDNPTQFVPAA; GDIPGNTQESTEEKIELLTTEVPLALEEESPSEGCPSSEIPMEKEPGEGRISVVDYSYLEGDLPISARPA; CSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQ; NFTATIQTAARIVPEEPSDSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFC; NSDDPYNPLNFKAPFQTSGENEKGCRDSETPSESIVAISECHTLLSCKVQLLGSQESECPDSVQRDVLSG; GRHTHVKRKKVTFLEEVTEYYISGDEDRKGPWEEFARDGCRFQKRIQETEDAIGYCLTFEHRERMFNRLQ; GTCFKGLNVLKQC; ; TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032833
• ORF Size: 2139 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032833.2, NP_116222.2
• RefSeq Size: 5264 bp
• RefSeq ORF: 2142 bp
• Locus ID: 84919
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome
• Summary: This gene encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism. [provided by RefSeq, Feb 2016]