TORC2 (CRTC2) (NM_181715) Human Tagged ORF Clone

SKU RG208836

CRTC2 (tGFP-tagged) - Human CREB regulated transcription coactivator 2 (CRTC2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TORC2
• Synonyms: TORC-2; TORC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG208836 representing NM_181715; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGACGTCGGGGGCGAACGGGCCTGGTTCGGCCACGGCCTCGGCTTCCAATCCGCGCAAATTTAGTG; AGAAGATTGCGCTGCAGAAGCAGCGTCAGGCCGAGGAGACGGCGGCCTTCGAGGAGGTGATGATGGACAT; CGGCTCCACCCGGTTACAGGCCCAAAAACTGCGACTGGCATACACAAGGAGCTCTCATTATGGTGGGTCT; CTGCCCAATGTTAACCAGATTGGCTCTGGCCTGGCCGAGTTCCAGAGCCCCCTCCACTCACCTTTGGATT; CATCTCGGAGCACTCGGCACCATGGGCTGGTGGAACGGGTGCAGCGAGATCCTCGAAGAATGGTGTCCCC; ACTTCGCCGATACACCCGCCACATTGACAGCTCTCCCTATAGTCCTGCCTACTTATCTCCTCCCCCAGAG; TCTAGCTGGCGAAGGACGATGGCCTGGGGCAATTTCCCTGCAGAGAAGGGGCAGTTGTTTCGACTACCAT; CTGCACTTAACAGGACAAGCTCTGACTCTGCCCTTCATACAAGTGTGATGAACCCCAGTCCCCAGGATAC; CTACCCAGGCCCCACACCTCCCAGCATCCTGCCCAGCCGACGTGGGGGTATTCTGGATGGTGAAATGGAC; CCCAAAGTACCTGCTATTGAGGAGAACTTGCTAGATGACAAGCATTTGCTGAAGCCATGGGATGCTAAGA; AGCTATCCTCATCCTCTTCCCGACCTCGGTCCTGTGAAGTCCCTGGAATTAACATCTTTCCATCTCCTGA; CCAGCCTGCCAATGTGCCTGTCCTCCCACCTGCCATGAACACGGGGGGCTCCCTACCTGACCTCACCAAC; CTGCACTTTCCCCCACCACTGCCCACCCCCCTGGACCCTGAAGAGACAGCCTACCCTAGCCTGAGTGGGG; GCAACAGTACCTCCAATTTGACCCACACCATGACTCACCTGGGCATCAGCAGGGGCATGGGCCTGGGCCC; AGGCTATGATGCACCAGGACTTCATTCACCTCTCAGCCACCCATCCCTGCAGTCCTCCCTAAGCAATCCC; AACCTCCAGGCTTCCCTGAGCAGTCCTCAGCCCCAGCTTCAGGGCTCCCACAGCCACCCCTCTCTGCCTG; CCTCCTCCTTGGCCCGCCATGTACTGCCCACCACCTCCCTGGGCCACCCCTCACTCAGTGCTCCGGCTCT; TTCCTCCTCCTCTTCCTCCTCCTCCACTTCATCTCCTGTTTTGGGCGCCCCCTCTTACCCTGCTTCTACC; CCTGGGGCCTCCCCCCACCACCGCCGTGTGCCCCTCAGCCCCCTGAGTTTGCTCGCGGGCCCAGCCGACG; CCAGAAGGTCCCAACAGCAGCTGCCCAAACAGTTTTCGCCAACAATGTCACCCACCTTGTCTTCCATCAC; TCAGGGCGTCCCCCTGGATACCAGTAAACTGTCCACTGACCAGCGGTTACCCCCATACCCATACAGCTCC; CCAAGTCTGGTTCTGCCTACCCAGCCCCACACCCCAAAGTCTCTACAGCAGCCAGGGCTGCCCTCTCAGT; CTTGTTCAGTGCAGTCCTCAGGTGGGCAGCCCCCAGGCAGGCAGTCTCATTATGGGACACCGTACCCACC; TGGGCCCAGTGGGCATGGGCAACAGTCTTACCACCGGCCAATGAGTGACTTCAACCTGGGGAATCTGGAG; CAGTTCAGCATGGAGAGCCCATCAGCCAGCCTGGTGCTGGATCCCCCTGGCTTTTCTGAAGGGCCTGGAT; TTTTAGGGGGTGAGGGGCCAATGGGTGGCCCCCAGGATCCCCACACCTTCAACCACCAGAACTTGACCCA; CTGTTCCCGCCATGGCTCAGGGCCTAACATCATCCTCACAGGGGACTCCTCTCCAGGTTTCTCTAAGGAG; ATTGCAGCAGCCCTGGCCGGAGTGCCTGGCTTTGAGGTGTCAGCAGCTGGATTGGAGCTAGGGCTTGGGC; TAGAAGATGAGCTGCGCATGGAGCCACTGGGCCTGGAAGGGCTAAACATGCTGAGTGACCCCTGTGCCCT; GCTGCCTGATCCTGCTGTGGAGGAGTCATTCCGCAGTGACCGGCTCCAA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG208836 representing NM_181715; Red=Cloning site Green=Tags(s); ; MATSGANGPGSATASASNPRKFSEKIALQKQRQAEETAAFEEVMMDIGSTRLQAQKLRLAYTRSSHYGGS; LPNVNQIGSGLAEFQSPLHSPLDSSRSTRHHGLVERVQRDPRRMVSPLRRYTRHIDSSPYSPAYLSPPPE; SSWRRTMAWGNFPAEKGQLFRLPSALNRTSSDSALHTSVMNPSPQDTYPGPTPPSILPSRRGGILDGEMD; PKVPAIEENLLDDKHLLKPWDAKKLSSSSSRPRSCEVPGINIFPSPDQPANVPVLPPAMNTGGSLPDLTN; LHFPPPLPTPLDPEETAYPSLSGGNSTSNLTHTMTHLGISRGMGLGPGYDAPGLHSPLSHPSLQSSLSNP; NLQASLSSPQPQLQGSHSHPSLPASSLARHVLPTTSLGHPSLSAPALSSSSSSSSTSSPVLGAPSYPAST; PGASPHHRRVPLSPLSLLAGPADARRSQQQLPKQFSPTMSPTLSSITQGVPLDTSKLSTDQRLPPYPYSS; PSLVLPTQPHTPKSLQQPGLPSQSCSVQSSGGQPPGRQSHYGTPYPPGPSGHGQQSYHRPMSDFNLGNLE; QFSMESPSASLVLDPPGFSEGPGFLGGEGPMGGPQDPHTFNHQNLTHCSRHGSGPNIILTGDSSPGFSKE; IAAALAGVPGFEVSAAGLELGLGLEDELRMEPLGLEGLNMLSDPCALLPDPAVEESFRSDRLQ; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181715
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_181715.1, NP_859066.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 2082 bp
• Locus ID: 200186
• UniProt ID: Q53ET0
• Cytogenetics: 1q21.3
• Summary: This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]