TORC2 (CRTC2) (NM_181715) Human Untagged Clone

SKU SC107319

CRTC2 (untagged)-Human CREB regulated transcription coactivator 2 (CRTC2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TORC2
• Synonyms: TORC-2; TORC2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107319 sequence for NM_181715 edited (data generated by NextGen Sequencing); ATGGCGACGTCGGGGGCGAACGGGCCTGGTTCGGCCACGGCCTCGGCTTCCAATCCGCGC; AAATTTAGTGAGAAGATTGCGCTGCAGAAGCAGCGTCAGGCCGAGGAGACGGCGGCCTTC; GAGGAGGTGATGATGGACATCGGCTCCACCCGGTTACAGGCCCAAAAACTGCGACTGGCA; TACACAAGGAGCTCTCATTATGGTGGGTCTCTGCCCAATGTTAACCAGATTGGCTCTGGC; CTGGCCGAGTTCCAGAGCCCCCTCCACTCACCTTTGGATTCATCTCGGAGCACTCGGCAC; CATGGGCTGGTGGAACGGGTGCAGCGAGATCCTCGAAGAATGGTGTCCCCACTTCGCCGA; TACACCCGCCACATTGACAGCTCTCCCTATAGTCCTGCCTACTTATCTCCTCCCCCAGAG; TCTAGCTGGCGAAGGACGGTGGCCTGGGGCAATTTCCCTGCAGAGAAGGGGCAGTTGTTT; CGACTACCATCTGCACTTAACAGGACAAGCTCTGACTCTGCCCTTCATACAAGTGTGATG; AACCCCAGTCCCCAGGATACCTACCCAGGCCCCACACCTCCCAGCATCCTGCCCAGCCGA; CGTGGGGGTATTCTGGATGGTGAAATGGACCCCAAAGTACCTGCTATTGAGGAGAACTTG; CTAGATGACAAGCATTTGCTGAAGCCATGGGATGCTAAGAAGCTATCCTCATCCTCTTCC; CGACCTCGGTCCTGTGAAGTCCCTGGAATTAACATCTTTCCATCTCCTGACCAGCCTGCC; AATGTGCCTGTCCTCCCACCTGCCATGAACACGGGGGGCTCCCTACCTGACCTCACCAAC; CTGCACTTTCCCCCACCACTGCCCACCCCCCTGGACCCTGAAGAGACAGCCTACCCTAGC; CTGAGTGGGGGCAACAGTACCTCCAATTTGACCCACACCATGACTCACCTGGGCATCAGC; AGGGGCATGGGCCTGGGCCCAGGCTATGATGCACCAGGACTTCATTCACCTCTCAGCCAC; CCATCCCTGCAGTCCTCCCTAAGCAATCCCAACCTCCAGGCTTCCCTGAGCAGTCCTCAG; CCCCAGCTTCAGGGCTCCCACAGCCACCCCTCTCTGCCTGCCTCCTCCTTGGCCCGCCAT; GTACTGCCCACCACCTCCCTGGGCCACCCCTCACTCAGTGCTCCGGCTCTCTCCTCCTCC; TCTTCCTCCTCCTCCACTTCATCTCCTGTTTTGGGCGCCCCCTCTTACCCTGCTTCTACC; CCTGGGGCCTCCCCCCACCACCGCCGTGTGCCCCTCAGCCCCCTGAGTTTGCTCGCGGGC; CCAGCCGACGCCAGAAGGTCCCAACAGCAGCTGCCCAAACAGTTTTCGCCAACAATGTCA; CCCACCTTGTCTTCCATCACTCAGGGCGTCCCCCTGGATACCAGTAAACTGTCCACTGAC; CAGCGGTTACCCCCATACCCATACAGCTCCCCAAGTCTGGTTCTGCCTACCCAGCCCCAC; ACCCCAAAGTCTCTACAGCAGCCAGGGCTGCCCTCTCAGTCTTGTTCAGTGCAGTCCTCA; GGTGGGCAGCCCCCAGGCAGGCAGTCTCATTATGGGACACCGTACCCACCTGGGCCCAGT; GGGCATGGGCAACAGTCTTACCACCGGCCAATGAGTGACTTCAACCTGGGGAATCTGGAG; CAGTTCAGCATGGAGAGCCCATCAGCCAGCCTGGTGCTGGATCCCCCTGGCTTTTCTGAA; GGGCCTGGATTTTTAGGGGGTGAGGGGCCAATGGGTGGCCCCCAGGATCCCCACACCTTC; AACCACCAGAACTTGACCCACTGTTCCCGCCATGGCTCAGGGCCTAACATCATCCTCACA; GGGGACTCCTCTCCAGGTTTCTCTAAGGAGATTGCAGCAGCCCTGGCCGGAGTGCCTGGC; TTTGAGGTGTCAGCAGCTGGATTGGAGCTAGGGCTTGGGCTAGAAGATGAGCTGCGCATG; GAGCCACTGGGCCTGGAAGGGCTAAACATGCTGAGTGACCCCTGTGCCCTGCTGCCTGAT; CCTGCTGTGGAGGAGTCATTCCGCAGTGACCGGCTCCAATGA; ; Clone variation with respect to NM_181715.2; 439 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_181715 unedited; AAATATAACACCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGGTCTAT; ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG; GCCGCGAATTCGGCACGAGGCTGGGGCCGGGTTCGCGGTGCTCGCTGAGGCGGCGGTGGC; TACGGCTGGAGGAGCCGGGCCGAGGCCGCGGCGGAGGCCGCGGCTGGTACTGGGAGGGTG; GCAGGGAGGGACGGGGAAGGAAGATGGCGACGTCGGGGGCGAACGGGCCTGGTTCGGCCA; CGGCCTCGGCTTCCAATCCGCGCAAATTTAGTGAGAAGATTGCGCTGCAGAAGCAGCGTC; AGGCCGAGGAGACGGCGGCCTTCGAGGAGGTGATGATGGACATCGGCTCCACCCGGTTAC; AGGCCCAAAAACTGCGACTGGCATACACAAGGAGCTCTCATTATGGTGGGTCTCTGCCCA; ATGTTAACCAGATTGGCTCTGGCCTGGCCGAGTTCCAGAGCCCCCTCCACTCACCTTTGG; ATTCATCTCGGAGCACTCGGCACCATGGGCTGGTGGAACGGGTGCAGCGAGATCCTCGAA; GAATGGTGTCCCCACTTCGCCGATACACCCGCCACATTGACAGCTCTCCCTATAGTCCTG; CCTACTTATCTCCTCCCCCAGAGTCTAGCTGGCGAAGGACGGTGGCCTGNGGCAATTTCC; CTGCAGAGAAGGGGCAGTTGTTTCGACTACCATCTGCACTTAACAGGACAAGCTCTGACT; CTGCCCTTCATACCAGTGTGATGAACCCCAGTCTCCAGGAACCTACCCAGGCCCCACACC; TCCCAGCATCCTGCCAGCCGACGTTGGGGGTATCCTTGGATGGTGCACACGGCCCCACAT; ACCTGCTATTGAG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_181715 unedited; NTTTGGGTTTCACTATGNNACCGCGGCCGCATNCTAGGATCGGATTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTGATATGATTTTTTTATTAACATATAATAATATATTTA; ATAAAAAATAGTATCCACTCTGGCTCTCTCCTCCACTGCAAGGGGAAAAATGGTGAGGAG; GGGCTGGCACTGCCCACCCCTAGGCATCCGGAAAAACCCTGGTTCCAGGGCTCCCCTCTA; CCCCTGCTTTCCAACAGGCTCTGACCTCCAGACAGACGGTTCAAAGTTACAAGTGCTTTA; GGCCCTCCCTTGAGTTCCCCCAGGCCCATCCCTTGCGCAAAATTCAGGGGCCACCTGGAC; AAACCCCTTGCTTTTTCTCATTCTTGGCATCCTTCATTCATGCTAAAAAGAGGATCTGGG; GACAGAAAGTAAAGTCTCTACCTGCCAGGGGGAAAGGAGGAAAAGAATGGTGGTGGGGGA; TGGGGCCCAAAAAGGGGATGGGGATGAGGGGCCCCCATTGGAACCGGGCACTGGGGAATG; ACTCCTCCCCAGCAAGATCAAGGAGCAGGGCACAAGGGTCACTCCACATGTTTAGCCCTT; TCAGGCCCAATGGCTCCATGCCCAACTTATTTTTTTGCCCAAACCCTAACTCCCATCCAG; GTTGGTGACACCCCAAAAGCCCGGCCCCTCCGGGCCCGGGGGTGGTTGCAATCTTCCTTA; AAGAAACCACCGAAGAAGGGAATCCCTTGGAAAGAAATGATTTAAGGCCCCTGGACCCTT; GCGCGGGAAACACTGGGGTGCAAATTCTTGGCGGGTTTAAGGGTGGGGGGGATCCTCGGG; GGGCCCACCCATTGGGCCCTCCACCCCCAAAAATACCACTCCCTCTCAGAAAAACCCCGG; GGGTACT;
• Restriction Sites: NotI-NotI
• ACCN: NM_181715
• Insert Size: 2750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_181715.1, NP_859066.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 2082 bp
• Locus ID: 200186
• UniProt ID: Q53ET0
• Cytogenetics: 1q21.3
• Summary: This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]