Alpha SNAP (NAPA) (NM_003827) Human Tagged ORF Clone

SKU RG207037

NAPA (tGFP-tagged) - Human N-ethylmaleimide-sensitive factor attachment protein, alpha (NAPA)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Alpha SNAP
• Synonyms: SNAPA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207037 representing NM_003827; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGACAATTCCGGGAAGGAAGCGGAGGCGATGGCGCTGTTGGCCGAGGCGGAGCGCAAAGTGAAGAACT; CGCAGTCCTTCTTCTCTGGCCTCTTTGGAGGCTCATCCAAAATAGAGGAAGCATGCGAAATCTACGCCAG; AGCAGCAAACATGTTCAAAATGGCCAAAAACTGGAGTGCTGCTGGAAACGCGTTCTGCCAGGCTGCACAG; CTGCACCTGCAGCTCCAGAGCAAGCACGACGCAGCCACCTGCTTTGTGGACGCTGGCAACGCATTCAAGA; AAGCCGACCCCCAAGAGGCCATTAACTGTTTGATGCGAGCAATCGAGATCTACACAGACATGGGCCGATT; CACGATTGCGGCCAAGCACCACATCTCCATTGCTGAGATCTATGAGACAGAGTTGGTGGACATCGAGAAG; GCCATTGCCCACTACGAGCAGTCTGCAGACTACTACAAAGGCGAGGAGTCCAACAGCTCAGCCAACAAGT; GTCTGCTGAAGGTGGCTGGTTACGCTGCGCTGCTGGAGCAGTATCAGAAGGCCATTGACATCTACGAACA; GGTGGGGACCAATGCCATGGACAGCCCCCTCCTCAAGTACAGCGCCAAAGACTACTTCTTCAAGGCGGCC; CTCTGCCACTTCTGCATCGACATGCTCAACGCCAAGCTGGCTGTCCAAAAGTATGAGGAGCTGTTCCCAG; CTTTCTCTGATTCCCGGGAATGCAAGTTGATGAAAAAATTGCTAGAGGCCCACGAGGAGCAGAATGTGGA; CAGCTACACCGAGTCGGTGAAGGAATACGACTCCATCTCCCGGCTGGACCAGTGGCTCACCACCATGCTG; CTGCGCATCAAGAAGACCATCCAGGGCGATGAGGAGGACCTGCGC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207037 representing NM_003827; Red=Cloning site Green=Tags(s); ; MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQ; LHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEK; AIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAA; LCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTML; LRIKKTIQGDEEDLR; ; SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003827
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003827.4
• RefSeq Size: 1846 bp
• RefSeq ORF: 888 bp
• Locus ID: 8775
• UniProt ID: P54920
• Cytogenetics: 19q13.32-q13.33
• Domains: NSF
• Summary: This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2011]