LASS3 (CERS3) (NM_178842) Human Tagged ORF Clone

SKU RG205511

CERS3 (tGFP-tagged) - Human LAG1 homolog, ceramide synthase 3 (LASS3)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: LASS3
• Synonyms: ARCI9; LASS3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205511 representing NM_178842; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAAAGATTCTGGCTTCCTCCAACAATAAAGTGGTCAG; ATCTTGAGGATCACGATGGACTCGTCTTTGTAAAACCTTCTCATTTATACGTGACAATTCCATATGCTTT; TCTCTTGCTGATTATCAGGCGTGTATTTGAAAAATTTGTTGCTTCACCTCTAGCAAAATCATTTGGCATT; AAAGAGACAGTTCGAAAGGTTACACCAAATACTGTCTTAGAGAATTTTTTCAAACATTCCACAAGGCAAC; CATTGCAAACTGATATTTATGGACTGGCAAAGAAGTGTAACTTGACGGAGCGCCAGGTGGAAAGATGGTT; TAGGAGTCGGCGGAATCAAGAGAGGCCTTCCAGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGATTTGCA; TTTTACTTAATGATCACTGTTGCTGGAATTGCGTTTCTTTATGATAAACCTTGGCTATATGACTTATGGG; AGGTTTGGAATGGCTATCCCAAACAGCCCCTGCTGCCATCCCAGTACTGGTACTACATTTTAGAAATGAG; TTTTTATTGGTCTCTGTTATTTAGACTTGGCTTTGATGTCAAGAGAAAGGATTTTCTAGCTCATATCATC; CACCACCTGGCTGCTATTAGTCTGATGAGCTTCTCTTGGTGTGCTAATTATATTCGCAGTGGGACCCTCG; TGATGATTGTACACGATGTGGCTGACATTTGGCTGGAGTCTGCTAAGATGTTTTCTTATGCTGGATGGAC; GCAGACCTGTAACACCCTGTTTTTCATCTTCTCCACCATATTTTTCATCAGCCGCCTCATTGTTTTTCCT; TTCTGGATTTTATATTGCACGCTGATCTTGCCTATGTATCACCTCGAGCCTTTCTTTTCATACATCTTCC; TCAACCTACAGCTCATGATCTTGCAGGTCCTTCACCTTTACTGGGGTTATTACATCTTGAAGATGCTCAA; CAGATGTATATTCATGAAGAGCATCCAGGATGTGAGGAGTGATGACGAGGATTATGAAGAGGAAGAGGAA; GAGGAAGAAGAAGAGGCTACCAAAGGCAAAGAGATGGATTGTTTAAAGAACGGCCTCGGGGCTGAGAGGC; ACCTCATTCCCAATGGCCAGCATGGCCAT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205511 representing NM_178842; Red=Cloning site Green=Tags(s); ; MFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIPYAFLLLIIRRVFEKFVASPLAKSFGI; KETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSRRNQERPSRLKKFQEACWRFA; FYLMITVAGIAFLYDKPWLYDLWEVWNGYPKQPLLPSQYWYYILEMSFYWSLLFRLGFDVKRKDFLAHII; HHLAAISLMSFSWCANYIRSGTLVMIVHDVADIWLESAKMFSYAGWTQTCNTLFFIFSTIFFISRLIVFP; FWILYCTLILPMYHLEPFFSYIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKSIQDVRSDDEDYEEEEE; EEEEEATKGKEMDCLKNGLGAERHLIPNGQHGH; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178842
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178842.2
• RefSeq Size: 3894 bp
• RefSeq ORF: 1152 bp
• Locus ID: 204219
• UniProt ID: Q8IU89
• Cytogenetics: 15q26.3
• Protein Families: Transcription Factors, Transmembrane
• Summary: This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]