Tbp7 (PSMC4) (NM_006503) Human Tagged ORF Clone

SKU RG204932

PSMC4 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Tbp7
• Synonyms: MIP224; RPT3; S6; TBP-7; TBP7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204932 representing NM_006503; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGGAGATAGGCATCTTGGTGGAGAAGGCTCAGGATGAGATCCCAGCACTGTCCGTGTCCCGGCCCC; AGACCGGCCTGTCCTTCCTGGGCCCTGAGCCTGAGGACCTGGAGGACCTGTACAGCCGCTACAAGAAGCT; GCAGCAAGAGCTGGAGTTCCTGGAGGTGCAGGAGGAATACATCAAAGATGAGCAAAAGAACCTGAAAAAG; GAATTTCTCCATGCCCAGGAGGAGGTGAAGCGAATCCAAAGCATCCCGCTGGTCATCGGACAATTTCTGG; AGGCTGTGGATCAGAATACAGCCATCGTGGGCTCTACCACAGGCTCCAACTATTATGTGCGCATCCTGAG; CACCATCGATCGGGAGCTGCTCAAGCCCAACGCCTCAGTGGCCCTCCACAAGCACAGCAATGCACTGGTG; GACGTGCTGCCCCCCGAAGCCGACAGCAGCATCATGATGCTCACCTCAGACCAGAAGCCAGATGTGATGT; ACGCGGACATCGGAGGCATGGACATCCAGAAGCAGGAGGTGCGGGAGGCCGTGGAGCTCCCGCTCACGCA; TTTCGAGCTCTACAAGCAGATCGGCATCGATCCCCCCCGAGGCGTCCTCATGTATGGCCCACCTGGCTGT; GGGAAGACCATGTTGGCAAAGGCGGTGGCACATCACACAACAGCTGCATTCATCCGGGTCGTGGGCTCGG; AGTTTGTACAGAAGTATCTGGGTGAGGGCCCCCGCATGGTCCGGGATGTGTTCCGCCTGGCCAAGGAGAA; TGCACCTGCCATCATCTTCATAGACGAGATTGATGCCATCGCCACCAAGAGATTCGATGCTCAGACAGGG; GCCGACAGGGAGGTTCAGAGGATCCTGCTGGAGCTGCTGAATCAGATGGATGGATTTGATCAGAATGTCA; ATGTCAAGGTAATCATGGCCACAAACAGAGCAGACACCCTGGATCCGGCCCTGCTACGGCCAGGACGGCT; GGACCGTAAAATTGAATTTCCACTTCCTGACCGCCGCCAGAAGAGATTGATTTTCTCCACTATCACTAGC; AAGATGAACCTCTCTGAGGAGGTTGACTTGGAAGACTATGTGGCCCGGCCAGATAAGATTTCAGGAGCTG; ATATTAACTCCATCTGTCAGGAGAGTGGAATGTTGGCTGTCCGTGAAAACCGCTACATTGTCCTGGCCAA; GGACTTCGAGAAAGCATACAAGACTGTCATCAAGAAGGACGAGCAGGAGCATGAGTTTTACAAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204932 representing NM_006503; Red=Cloning site Green=Tags(s); ; MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKK; EFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALV; DVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGC; GKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTG; ADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITS; KMNLSEEVDLEDYVARPDKISGADINSICQESGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006503
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006503.4
• RefSeq Size: 1450 bp
• RefSeq ORF: 1257 bp
• Locus ID: 5704
• UniProt ID: P43686
• Cytogenetics: 19q13.2
• Domains: AAA
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]