PIGK (NM_005482) Human Tagged ORF Clone

SKU RG204728

PIGK (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class K (PIGK)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PIGK
• Synonyms: GPI8; NEDHCAS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204728 representing NM_005482; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCGTCACCGACAGCCTCAGCCGGGCTGCGACTGTCTTGGCAACTGTGTTGCTCTTGTCCTTCGGCA; GCGTGGCCGCTAGTCATATCGAGGATCAAGCAGAACAATTCTTTAGAAGTGGCCATACAAACAACTGGGC; TGTTCTGGTGTGTACATCCCGATTCTGGTTTAATTATCGACATGTTGCAAATACCCTTTCTGTTTATAGA; AGTGTCAAGAGGCTAGGTATTCCTGACAGTCACATTGTCCTAATGCTTGCAGATGATATGGCCTGTAATC; CTAGAAATCCCAAACCAGCTACAGTGTTTAGTCACAAGAATATGGAACTAAATGTGTATGGAGATGATGT; GGAAGTGGATTATAGAAGTTATGAGGTAACTGTGGAGAATTTTTTACGGGTATTAACTGGGAGGATCCCA; CCTAGTACTCCTCGGTCAAAACGTCTTCTTTCTGATGACAGAAGCAATATTCTAATTTATATGACAGGGC; ATGGTGGAAATGGTTTCTTAAAATTTCAAGATTCTGAAGAAATTACCAACATAGAACTCGCGGATGCTTT; TGAACAAATGTGGCAGAAAAGACGCTACAATGAGCTACTGTTTATTATTGATACTTGCCAAGGAGCATCC; ATGTATGAACGATTTTATTCTCCTAACATAATGGCTCTAGCTAGTAGTCAAGTGGGAGAAGATTCACTCT; CGCATCAACCTGATCCTGCAATTGGAGTCCATCTTATGGATAGATACACATTTTATGTCTTGGAATTTTT; GGAAGAAATTAACCCAGCTAGCCAAACTAATATGAATGACCTTTTTCAGGTATGTCCCAAAAGTCTGTGT; GTGTCTACTCCTGGACATCGCACTGATCTTTTTCAGAGGGATCCTAAAAATGTACTGATAACTGATTTCT; TTGGAAGTGTACGGAAAGTGGAAATTACAACAGAGACTATTAAATTGCAACAGGATTCAGAAATCATGGA; AAGCAGCTATAAGGAAGACCAGATGGATGAGAAACTAATGGAACCTCTGAAATATGCTGAACAACTTCCT; GTAGCTCAGATAATACACCAGAAACCGAAGCTGAAAGACTGGCATCCTCCTGGGGGCTTTATTCTGGGAT; TATGGGCACTTATTATCATGGTTTTCTTCAAAACTTATGGAATTAAGCATATGAAGTTCATTTTT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204728 representing NM_005482; Red=Cloning site Green=Tags(s); ; MAVTDSLSRAATVLATVLLLSFGSVAASHIEDQAEQFFRSGHTNNWAVLVCTSRFWFNYRHVANTLSVYR; SVKRLGIPDSHIVLMLADDMACNPRNPKPATVFSHKNMELNVYGDDVEVDYRSYEVTVENFLRVLTGRIP; PSTPRSKRLLSDDRSNILIYMTGHGGNGFLKFQDSEEITNIELADAFEQMWQKRRYNELLFIIDTCQGAS; MYERFYSPNIMALASSQVGEDSLSHQPDPAIGVHLMDRYTFYVLEFLEEINPASQTNMNDLFQVCPKSLC; VSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQMDEKLMEPLKYAEQLP; VAQIIHQKPKLKDWHPPGGFILGLWALIIMVFFKTYGIKHMKFIF; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005482
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005482.2, NP_005473.1
• RefSeq Size: 4626 bp
• RefSeq ORF: 1188 bp
• Locus ID: 10026
• UniProt ID: Q92643
• Cytogenetics: 1p31.1
• Domains: Peptidase_C13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Summary: This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]