PIGK (NM_005482) Human Untagged Clone

SKU SC101219

PIGK (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class K (PIGK)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PIGK
• Synonyms: GPI8; NEDHCAS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101219 sequence for NM_005482 edited (data generated by NextGen Sequencing); ATGGCCGTCACCGACAGCCTCAGCCGGGCTGCGACTGTCTTGGCAACTGTGTTGCTCTTG; TCCTTCGGCAGCGTGGCCGCTAGTCATATCGAGGATCAAGCAGAACAATTCTTTAGAAGT; GGCCATACAAACAACTGGGCTGTTCTGGTGTGTACATCCCGATTCTGGTTTAATTATCGA; CATGTTGCAAATACCCTTTCTGTTTATAGAAGTGTCAAGAGGCTAGGTATTCCTGACAGT; CACATTGTCCTAATGCTTGCAGATGATATGGCCTGTAATCCTAGAAATCCCAAACCAGCT; ACAGTGTTTAGTCACAAGAATATGGAACTAAATGTGTATGGAGATGATGTGGAAGTGGAT; TATAGAAGTTATGAGGTAACTGTGGAGAATTTTTTACGGGTATTAACTGGGAGGATCCCA; CCTAGTACTCCTCGGTCAAAACGTCTTCTTTCTGATGACAGAAGCAATATTCTAATTTAT; ATGACAGGGCATGGTGGAAATGGTTTCTTAAAATTTCAAGATTCTGAAGAAATTACCAAC; ATAGAACTCGCGGATGCTTTTGAACAAATGTGGCAGAAAAGACGCTACAATGAGCTACTG; TTTATTATTGATACTTGCCAAGGAGCATCCATGTATGAACGATTTTATTCTCCTAACATA; ATGGCTCTAGCTAGTAGTCAAGTGGGAGAAGATTCACTCTCGCATCAACCTGATCCTGCA; ATTGGAGTCCATCTTATGGATAGATACACATTTTATGTCTTGGAATTTTTGGAAGAAATT; AACCCAGCTAGCCAAACTAATATGAATGACCTTTTTCAGGTATGTCCCAAAAGTCTGTGT; GTGTCTACTCCTGGACATCGCACTGATCTTTTTCAGAGGGATCCTAAAAATGTACTGATA; ACTGATTTCTTTGGAAGTGTACGGAAAGTGGAAATTACAACAGAGACTATTAAATTGCAA; CAGGATTCAGAAATCATGGAAAGCAGCTATAAGGAAGACCAGATGGATGAGAAACTAATG; GAACCTCTGAAATATGCTGAACAACTTCCTGTAGCTCAGATAATACACCAGAAACCGAAG; CTGAAAGACTGGCATCCTCCTGGGGGCTTTATTCTGGGATTATGGGCACTTATTATCATG; GTTTTCTTCAAAACTTATGGAATTAAGCATATGAAGTTCATTTTTTAG; ; Clone variation with respect to NM_005482.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005482 unedited; GTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTGAAGCCGGTA; AACATGGCCGTCACCGACAGCCTCAGCCGGGCTGCGACTGTCTTGGCAACTGTGTTGCTC; TTGTCCTTCGGCAGCGTGGCCGCTAGTCATATCGAGGATCAAGCAGAACAATTCTTTAGA; AGTGGCCATACAAACAACTGGGCTGTTCTGGTGTGTACATCCCGATTCTGGTTTAATTAT; CGACATGTTGCAAATACCCTTTCTGTTTATAGAAGTGTCAAGAGGCTAGGTATTCCTGAC; AGTCACATTGTCCTAATGCTTGCAGATGATATGGCCTGTAATCCTAGAAATCCCAAACCA; GCTACAGTGTTTAGTCACAAGAATATGGAACTAAATGTGTATGGAGATGATGTGGAAGTG; GATTATAGAAGTTATGAGGTAACTGTGGAGAATTTTTTACGGGTATTAACTGGGAGGATC; CCACCTAGTACTCCTCGGTCAAAACGTCTTCTTTCTGATGACAGAAGCAATATTCTAATT; TATATGACAGGGCATGGTGGAAATGGTTTCTTAAAATTTCAAGATTCTGAAGAAATTACC; AACATAGAACTCGCGGATGCTTTTGAACAAATGTGGCAGAAAAGACGCTACAATGAGCTA; CTGTTTATTATTGATACTTGCCAAGGAGCATCCATGTATGAACGATTTTATTCTCCTAAC; ATAATGGCTCTAGCTAGTAGTCAAGTGGGAGAAGATTCACTCTCGCATCAACCTGATCCT; GNCATTGGAGTCCATCTTATGGATAGATACACATTNTATGTCTTGGAATTTTGGNAAGAA; ATAACCCAGCTAGCCAAACTAATATGAATGACCTTNNTCAGGTATGTCCCANAAGTCTTG; TGTGTGTCTACTCCTGGCATCGCACTGATCTTTTNCANAGGGATCCTAAAATGTACTGGA; TACTGATTCTTTTGGAAGGN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005482 unedited; AATTTACTTGNACCGCGGCCCGCTTNCTAGNGATCGATTTTTTTTTTTTTTTTTTCATCT; TTGCTTTTAATTAAAGCTGCAGGATTCCATGAGTGACCAGCAGTAAAATATTAATACATT; AAAGAATAAACCAAAGGAAAACATTTGGTACAATGTAAGTGGTCCAACAATAGAAGACTG; TGAAATGAATTAAATATTCATAAAGTGAATACTATGCAGTCATTCACAATGTATTTTTGA; GAGGGAAAAGGTTGATAATACATTGTAAAATGAAAAAAGACATTACAAAATAGTACATTC; CATATGATCTCCATATTGGGAAAAAATATGTGCATAAGTCAAAAGATTGGGAGGATATAC; AAAAAATAATTAACCAAAGTTACTTCTGTTAGTGAGAATTTAATGTCTTCACAATTGTTT; CTTTTCCAAATGAAAAGGGGATAAGGGGAAATTACTATTTTCAAAAACAGTCAATGTATT; CTGATCTCTGTCTCATTCGCTTTTATAATACACAAAATACAAAACTGGAATATATTTAGT; GCCATTAAGCAGTTGCATTAACAATTATTTTTCTTTAAGTTATAAAATTAATAGTTGATT; CAAATTTAGTTTCCTTATACTTATTTCCAATTCATACAAGAGAAACACATTTTTAAAAAT; ATATAATGACATAAATTATTATCCAAGTTTGCAGTCCCTCATGCATTCTTCATTCATCAT; CAACTCTAAAAAATGAACTTCATATGCTTAATTCCATAAGTTTTGAAGAAAACCATGATA; ATAAGTGCCCATAATCCCAGAATAAAGCCCCCAGGAGGATGCCAGTCTTTCAGCTTCGGT; TTCTGGTGTATTATCTGAGCTACAGGAAGTTTGTCCACCATATTTCAGAGTTTCCCATAG; TTTCCTCACCCATCTGGGTCTTCCTTATAGCTGCTTTTCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005482
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: A TrueClone.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005482.1, NP_005473.1
• RefSeq Size: 1897 bp
• RefSeq ORF: 1188 bp
• Locus ID: 10026
• UniProt ID: Q92643
• Cytogenetics: 1p31.1
• Domains: Peptidase_C13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Summary: This gene encodes a member of the cysteine protease family C13 that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is a member of the multisubunit enzyme, GPI transamidase and is thought to be its enzymatic component. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008]