GMP Synthase (GMPS) (NM_003875) Human Tagged ORF Clone

SKU RG204267

GMPS (tGFP-tagged) - Human guanine monphosphate synthetase (GMPS)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GMP Synthase
• Synonyms: GATD7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204267 representing NM_003875; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTCTGTGCAACGGAGACTCCAAGCTGGAGAATGCTGGAGGAGACCTTAAGGATGGCCACCACCACT; ATGAAGGAGCTGTTGTCATTCTGGATGCTGGTGCTCAGTACGGGAAAGTCATAGACCGAAGAGTGAGGGA; ACTGTTCGTGCAGTCTGAAATTTTCCCCTTGGAAACACCAGCATTTGCTATAAAGGAACAAGGATTCCGT; GCTATTATCATCTCTGGAGGACCTAATTCTGTGTATGCTGAAGATGCTCCCTGGTTTGATCCAGCAATAT; TCACTATTGGCAAGCCTGTTCTTGGAATTTGCTATGGTATGCAGATGATGAATAAGGTATTTGGAGGTAC; TGTGCACAAAAAAAGTGTCAGAGAAGATGGAGTTTTCAACATTAGTGTGGATAATACATGTTCATTATTC; AGGGGCCTTCAGAAGGAAGAAGTTGTTTTGCTTACACATGGAGATAGTGTAGACAAAGTAGCTGATGGAT; TCAAGGTTGTGGCACGTTCTGGAAACATAGTAGCAGGCATAGCAAATGAATCTAAAAAGTTATATGGAGC; ACAGTTCCACCCTGAAGTTGGCCTTACAGAAAATGGAAAAGTAATACTGAAGAATTTCCTTTATGATATA; GCTGGATGCAGTGGAACCTTCACCGTGCAGAACAGAGAACTTGAGTGTATTCGAGAGATCAAAGAGAGAG; TAGGCACGTCAAAAGTTTTGGTTTTACTCAGTGGTGGAGTAGACTCAACAGTTTGTACAGCTTTGCTAAA; TCGTGCTTTGAACCAAGAACAAGTCATTGCTGTGCACATTGATAATGGCTTTATGAGAAAACGAGAAAGC; CAGTCTGTTGAAGAGGCCCTCAAAAAGCTTGGAATTCAGGTCAAAGTGATAAATGCTGCTCATTCTTTCT; ACAATGGAACAACAACCCTACCAATATCAGATGAAGATAGAACCCCACGGAAAAGAATTAGCAAAACGTT; AAATATGACCACAAGTCCTGAAGAGAAAAGAAAAATCATTGGGGATACTTTTGTTAAGATTGCCAATGAA; GTAATTGGAGAAATGAACTTGAAACCAGAGGAGGTTTTCCTTGCCCAAGGTACTTTACGGCCTGATCTAA; TTGAAAGTGCATCCCTTGTTGCAAGTGGCAAAGCTGAACTCATCAAAACCCATCACAATGACACAGAGCT; CATCAGAAAGTTGAGAGAGGAGGGAAAAGTAATAGAACCTCTGAAAGATTTTCATAAAGATGAAGTGAGA; ATTTTGGGCAGAGAACTTGGACTTCCAGAAGAGTTAGTTTCCAGGCATCCATTTCCAGGTCCTGGCCTGG; CAATCAGAGTAATATGTGCTGAAGAACCTTATATTTGTAAGGACTTTCCTGAAACCAACAATATTTTGAA; AATAGTAGCTGATTTTTCTGCAAGTGTTAAAAAGCCACATACCCTATTACAGAGAGTCAAAGCCTGCACA; ACAGAAGAGGATCAGGAGAAGCTGATGCAAATTACCAGTCTGCATTCACTGAATGCCTTCTTGCTGCCAA; TTAAAACTGTAGGTGTGCAGGGTGACTGTCGTTCCTACAGTTACGTGTGTGGAATCTCCAGTAAAGATGA; ACCTGACTGGGAATCACTTATTTTTCTGGCTAGGCTTATACCTCGCATGTGTCACAACGTTAACAGAGTT; GTTTATATATTTGGCCCACCAGTTAAAGAACCTCCTACAGATGTTACTCCCACTTTCTTGACAACAGGGG; TGCTCAGTACTTTACGCCAAGCTGATTTTGAGGCCCATAACATTCTCAGGGAGTCTGGGTATGCTGGGAA; AATCAGCCAGATGCCGGTGATTTTGACACCATTACATTTTGATCGGGACCCACTTCAAAAGCAGCCTTCA; TGCCAGAGATCTGTGGTTATTCGAACCTTTATTACTAGTGACTTCATGACTGGTATACCTGCAACACCTG; GCAATGAGATCCCTGTAGAGGTGGTATTAAAGATGGTCACTGAGATTAAGAAGATTCCTGGTATTTCTCG; AATTATGTATGACTTAACATCAAAGCCCCCAGGAACTACTGAGTGGGAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204267 representing NM_003875; Red=Cloning site Green=Tags(s); ; MALCNGDSKLENAGGDLKDGHHHYEGAVVILDAGAQYGKVIDRRVRELFVQSEIFPLETPAFAIKEQGFR; AIIISGGPNSVYAEDAPWFDPAIFTIGKPVLGICYGMQMMNKVFGGTVHKKSVREDGVFNISVDNTCSLF; RGLQKEEVVLLTHGDSVDKVADGFKVVARSGNIVAGIANESKKLYGAQFHPEVGLTENGKVILKNFLYDI; AGCSGTFTVQNRELECIREIKERVGTSKVLVLLSGGVDSTVCTALLNRALNQEQVIAVHIDNGFMRKRES; QSVEEALKKLGIQVKVINAAHSFYNGTTTLPISDEDRTPRKRISKTLNMTTSPEEKRKIIGDTFVKIANE; VIGEMNLKPEEVFLAQGTLRPDLIESASLVASGKAELIKTHHNDTELIRKLREEGKVIEPLKDFHKDEVR; ILGRELGLPEELVSRHPFPGPGLAIRVICAEEPYICKDFPETNNILKIVADFSASVKKPHTLLQRVKACT; TEEDQEKLMQITSLHSLNAFLLPIKTVGVQGDCRSYSYVCGISSKDEPDWESLIFLARLIPRMCHNVNRV; VYIFGPPVKEPPTDVTPTFLTTGVLSTLRQADFEAHNILRESGYAGKISQMPVILTPLHFDRDPLQKQPS; CQRSVVIRTFITSDFMTGIPATPGNEIPVEVVLKMVTEIKKIPGISRIMYDLTSKPPGTTEWE; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003875
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003875.3
• RefSeq Size: 2457 bp
• RefSeq ORF: 2082 bp
• Locus ID: 8833
• UniProt ID: P49915
• Cytogenetics: 3q25.31
• Domains: GATase, GMP_synt_C
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Summary: In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]