GMP Synthase (GMPS) (NM_003875) Human Untagged Clone

SKU SC117688

GMPS (untagged)-Human guanine monphosphate synthetase (GMPS)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GMP Synthase
• Synonyms: GATD7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117688 sequence for NM_003875 edited (data generated by NextGen Sequencing); ATGGCTCTGTGCAACGGAGACTCCAAGCTGGAGAATGCTGGAGGAGACCTTAAGGATGGC; CACCACCACTATGAAGGAGCTGTTGTCATTCTGGATGCTGGTGCTCAGTACGGGAAAGTC; ATAGACCGAAGAGTGAGGGAACTGTTCGTGCAGTCTGAAATTTTCCCCTTGGAAACACCA; GCATTTGCTATAAAGGAACAAGGATTCCGTGCTATTATCATCTCTGGAGGACCTAATTCT; GTGTATGCTGAAGATGCTCCCTGGTTTGATCCAGCAATATTCACTATTGGCAAGCCTGTT; CTTGGAATTTGCTATGGTATGCAGATGATGAATAAGGTATTTGGAGGTACTGTGCACAAA; AAAAGTGTCAGAGAAGATGGAGTTTTCAACATTAGTGTGGATAATACATGTTCATTATTC; AGGGGCCTTCAGAAGGAAGAAGTTGTTTTGCTTACACATGGAGATAGTGTAGACAAAGTA; GCTGATGGATTCAAGGTTGTGGCACGTTCTGGAAACATAGTAGCAGGCATAGCAAATGAA; TCTAAAAAGTTATATGGAGCACAGTTCCACCCTGAAGTTGGCCTTACAGAAAATGGAAAA; GTAATACTGAAGAATTTCCTTTATGATATAGCTGGATGCAGTGGAACCTTCACCGTGCAG; AACAGAGAACTTGAGTGTATTCGAGAGATCAAAGAGAGAGTAGGCACGTCAAAAGTTTTG; GTTTTACTCAGTGGTGGAGTAGACTCAACAGTTTGTACAGCTTTGCTAAATCGTGCTTTG; AACCAAGAACAAGTCATTGCTGTGCACATTGATAATGGCTTTATGAGAAAACGAGAAAGC; CAGTCTGTTGAAGAGGCCCTCAAAAAGCTTGGAATTCAGGTCAAAGTGATAAATGCTGCT; CATTCTTTCTACAATGGAACAACAACCCTACCAATATCAGATGAAGATAGAACCCCACGG; AAAAGAATTAGCAAAACGTTAAATATGACCACAAGTCCTGAAGAGAAAAGAAAAATCATT; GGGGATACTTTTGTTAAGATTGCCAATGAAGTAATTGGAGAAATGAACTTGAAACCAGAG; GAGGTTTTCCTTGCCCAAGGTACTTTACGGCCTGATCTAATTGAAAGTGCATCCCTTGTT; GCAAGTGGCAAAGCTGAACTCATCAAAACCCATCACAATGACACAGAGCTCATCAGAAAG; TTGAGAGAGGAGGGAAAAGTAATAGAACCTCTGAAAGATTTTCATAAAGATGAAGTGAGA; ATTTTGGGCAGAGAACTTGGACTTCCAGAAGAGTTAGTTTCCAGGCATCCATTTCCAGGT; CCTGGCCTGGCAATCAGAGTAATATGTGCTGAAGAACCTTATATTTGTAAGGACTTTCCT; GAAACCAACAATATTTTGAAAATAGTAGCTGATTTTTCTGCAAGTGTTAAAAAGCCACAT; ACCCTATTACAGAGAGTCAAAGCCTGCACAACAGAAGAGGATCAGGAGAAGCTGATGCAA; ATTACCAGTCTGCATTCACTGAATGCCTTCTTGCTGCCAATTAAAACTGTAGGTGTGCAG; GGTGACTGTCGTTCCTACAGTTACGTGTGTGGAATCTCCAGTAAAGATGAACCTGACTGG; GAATCACTTATTTTTCTGGCTAGGCTTATACCTCGCATGTGTCACAACGTTAACAGAGTT; GTTTATATATTTGGCCCACCAGTTAAAGAACCTCCTACAGATGTTACTCCCACTTTCTTG; ACAACAGGGGTGCTCAGTACTTTACGCCAAGCTGATTTTGAGGCCCATAACATTCTCAGG; GAGTCTGGGTATGCTGGGAAAATCAGCCAGATGCCGGTGATTTTGACACCATTACATTTT; GATCGGGACCCACTTCAAAAGCAGCCTTCATGCCAGAGATCTGTGGTTATTCGAACCTTT; ATTACTAGTGACTTCATGACTGGTATACCTGCAACACCTGGCAATGAGATCCCTGTAGAG; GTGGTATTAAAGATGGTCACTGAGATTAAGAAGATTCCTGGTATTTCTCGAATTATGTAT; GACTTAACATCAAAGCCCCCAGGAACTACTGAGTGGGAGTAA; ; Clone variation with respect to NM_003875.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003875 unedited; GGTTGTCACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTGGT; CTTCTCTCCCGCGGCGCTGGGGCCCGCGCTCCGCTGCTGTTGCTCCATTCGGCGCTTTTC; TGGCGGCTGGCTCCTCTCCGCTGCCGGCTGCTCCTCGACCAGGCCTCCTTCTCAACCTCA; GCCCGCGGCGCCGACCCTTCCGGCACCCTCCCGCCCCGTCTCGTACTGTCGCCGTCACCG; CCGCGGCTCCGGCCCTGGCCCCGATGGCTCTGTGCAACGGAGACTCCAAGCTGGAGAATG; CTGGAGGAGACCTTAAGGATGGCCACCACCACTATGAAGGAGCTGTTGTCATTCTGGATG; CTGGTGCTCAGTACGGGAAAGTCATAGACCGAAGAGTGAGGGAACTGTTCGTGCAGTCTG; AAATTTTCCCCTTGGAAACACCAGCATTTGCTATAAAGGAACAAGGATTCCGTGCTATTA; TCATCTCTGGAGGACCTAATTCTGTGTATGCTGAAGATGCTCCCTGGTTTGATCCAGCAA; TATTCACTATTGGCAAGCCTGTTCTTGGAATTTGCTATGGTATGCAGATGATGAATAAGG; TATTTGGAGGTACTGTGCACAAAAAAAGTGTCAGAGAAGATGGAGTTTTCAACATTAGTG; TGGATAATACATGTTCATTATTCAGGGGCCTTCAGAAGGAAGAAGTTGTTTTGCTTACAC; ATGGAGATAGTGTAGACAAAGTAGCTGATGGATTCAAGGTTGTGGCACGTTCTGGAAACA; TAGTAGCAGGCATAGCANATGAATCTAAAAAGTTATATGGGAGCACAGTTNCACCCCTGA; AGNTGGCCTTACANGAAATGGNNAAAGTATACTGAAGAATTTCCTTTATGATATAGCTGG; ATGCAGTGGAACCTTCACGTGCAGAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003875 unedited; GTACCGCGGCACGCAATCTAGTGTCGAGTTTTTTTTTTTTTTTTTTACTTTAATAGAACA; AGAAGTTTATTACTCCCACTCAGTAGTTCCTGGGGGCTTTGATGTTAAGTCATACATAAT; TCGAGAAATACCAGGAATCTTCTTAATCTCAGTGACCATCTTTAATACCACCTCTACAGG; GATCTCATTGCCAGGTGTTGCAGGTATACCAGTCATGAAGTCACTAGTAATAAAGGTTCG; AATAACCACAGATCTCTGGCATGAAAGCTGCTTTTGAAGTGGGTCCCGATCAAAATGTAA; TGGTGTCAAAATCACCGGCATCTGGCTGATTTTCCCAGCATACCCAGACTCCCTGAGAAT; GTTATGGGCCTCAAAATCAGCTTGGCGTAAAGTACTGAGCACCCCTGTTGTCAAGATAGT; GGGAGTAACATCTGTAAGAGGTTCTTTAACTGGTGGGCCAAATATATAAACAACTCTGTT; AACGTTGTGACACATGCGATGTATAAGCCTAGCCAGATAATAAGTGATTCCCAGTCAGGT; TCATCTTTACTGGAGATTCCACACACGTAACTGTAAGAACGACAGTCACCCTGCACACCT; ACAGTTNTAATTGGCAGCAAGATAGCATTCAGTGAATGCAGACTGGTAATTTGCATCAGC; TTCTCCTGATCCTCTTCTAGTGTGCAAGCTTTGACTCTCTGTAATAAGGTATGTAGGCTT; TTTAACACTTGCAGAAAATCAGCTACTATTTTCAAATATTGGTAGGTTCAGGAAAATCCT; TACAATATAAAGTNTTCAGCACATATTACTCTGATTGCCANGCCAGGACCCTGGAATGGA; TGCCCTGGAACTAACTCTTCTGGAAGTCCAGTTCTCTGCCCAAATTCCACCTCATCTTAT; GAAAATCTTCAAAGTNCTATTACTTTTCCCTCCTCTCTAACTTCTGATGGCTCTGGGCCA; TGGGATGGCCTTTGAGAGNTCACTCTTGCACCGGCAACAGGGATGCACTTNNCATANATC; AGGCCGTAAGACN;
• Restriction Sites: NotI-NotI
• ACCN: NM_003875
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003875.2, NP_003866.1
• RefSeq Size: 2457 bp
• RefSeq ORF: 2082 bp
• Locus ID: 8833
• UniProt ID: P49915
• Cytogenetics: 3q25.31
• Domains: GATase, GMP_synt_C
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Summary: In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]