PSTPIP1 (NM_003978) Human Tagged ORF Clone

SKU RG203788

PSTPIP1 (tGFP-tagged) - Human proline-serine-threonine phosphatase interacting protein 1 (PSTPIP1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PSTPIP1
• Synonyms: CD2BP1; CD2BP1L; CD2BP1S; H-PIP; PAPAS; PSTPIP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203788 representing NM_003978; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGCCCCAGCTGCAGTTCAAAGATGCCTTTTGGTGCAGGGACTTCACAGCCCACACGGGCTACGAGG; TGCTGCTGCAGCGGCTTCTGGATGGCAGGAAGATGTGCAAAGACATGGAGGAGCTACTGAGGCAGAGGGC; CCAGGCGGAGGAGCGGTACGGGAAGGAGCTGGTGCAGATCGCACGGAAGGCAGGTGGCCAGACGGAGATC; AACTCCCTGAGGGCCTCCTTTGACTCCTTGAAGCAGCAAATGGAGAATGTGGGCAGCTCACACATCCAGC; TGGCCCTGACCCTGCGTGAGGAGCTGCGGAGTCTCGAGGAGTTTCGTGAGAGGCAGAAGGAGCAGAGGAA; GAAGTATGAGGCCGTCATGGACCGGGTCCAGAAGAGCAAGCTGTCGCTCTACAAGAAGGCCATGGAGTCC; AAGAAGACATACGAGCAGAAGTGCCGGGACGCGGACGACGCGGAGCAGGCCTTCGAGCGCATTAGCGCCA; ACGGCCACCAGAAGCAGGTGGAGAAGAGTCAGAACAAAGCCAGGCAGTGCAAGGACTCGGCCACCGAGGC; AGAGCGGGTATACAGGCAGAGCATTGCGCAGCTGGAGAAGGTCCGGGCTGAGTGGGAGCAGGAGCACCGG; ACCACCTGTGAGGCCTTTCAGCTGCAAGAGTTTGACCGGCTGACCATTCTCCGCAACGCCCTGTGGGTGC; ACAGCAACCAGCTCTCCATGCAGTGTGTCAAGGATGATGAGCTCTACGAGGAAGTGCGGCTGACGCTGGA; AGGCTGCAGCATAGACGCCGACATCGACAGTTTCATCCAGGCCAAGAGCACGGGCACAGAGCCCCCCGCT; CCGGTGCCCTACCAGAACTATTACGATCGGGAGGTCACCCCGCTGACCAGCAGCCCTGGCATACAGCCGT; CCTGCGGCATGATAAAGAGGTTCTCTGGACTGCTGCACGGAAGTCCCAAGACCACTTCGTTGGCAGCTTC; TGCTGCGTCCACAGAGACCCTGACCCCCACCCCCGAGCGGAATGAGGGTGTCTACACAGCCATCGCAGTG; CAGGAGATACAGGGAAACCCGGCCTCACCAGCCCAGGAGTACCGGGCGCTCTACGATTATACAGCGCAGA; ACCCAGATGAGCTGGACCTGTCCGCGGGAGACATCCTGGAGGTGATCCTGGAAGGGGAGGATGGCTGGTG; GACTGTGGAGAGGAACGGGCAGCGTGGCTTCGTCCCTGGTTCCTACCTGGAGAAGCTT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203788 representing NM_003978; Red=Cloning site Green=Tags(s); ; MMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEI; NSLRASFDSLKQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMES; KKTYEQKCRDADDAEQAFERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHR; TTCEAFQLQEFDRLTILRNALWVHSNQLSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPA; PVPYQNYYDREVTPLTSSPGIQPSCGMIKRFSGLLHGSPKTTSLAASAASTETLTPTPERNEGVYTAIAV; QEIQGNPASPAQEYRALYDYTAQNPDELDLSAGDILEVILEGEDGWWTVERNGQRGFVPGSYLEKL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003978
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003978.5
• RefSeq Size: 1781 bp
• RefSeq ORF: 1251 bp
• Locus ID: 9051
• UniProt ID: O43586
• Cytogenetics: 15q24.3
• Domains: FCH, SH3
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• Summary: This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response. [provided by RefSeq, Mar 2016]