PSTPIP1 (NM_003978) Human Untagged Clone

SKU SC117638

PSTPIP1 (untagged)-Human proline-serine-threonine phosphatase interacting protein 1 (PSTPIP1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PSTPIP1
• Synonyms: CD2BP1; CD2BP1L; CD2BP1S; H-PIP; PAPAS; PSTPIP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003978, the custom clone sequence may differ by one or more nucleotides; ; ; ATGATGCCCCAGCTGCAGTTCAAAGATGCCTTTTGGTGCAGGGACTTCACAGCCCACACGGGCTACGAGG; TGCTGCTGCAGCGGCTTCTGGATGGCAGGAAGATGTGCAAAGACATGGAGGAGCTACTGAGGCAGAGGGC; CCAGGCGGAGGAGCGGTACGGGAAGGAGCTGGTGCAGATCGCACGGAAGGCAGGTGGCCAGACGGAGATC; AACTCCCTGAGGGCCTCCTTTGACTCCTTGAAGCAGCAAATGGAGAATGTGGGCAGCTCACACATCCAGC; TGGCCCTGACCCTGCGTGAGGAGCTGCGGAGTCTCGAGGAGTTTCGTGAGAGGCAGAAGGAGCAGAGGAA; GAAGTATGAGGCCGTCATGGACCGGGTCCAGAAGAGCAAGCTGTCGCTCTACAAGAAGGCCATGGAGTCC; AAGAAGACATACGAGCAGAAGTGCCGGGACGCGGACGACGCGGAGCAGGCCTTCGAGCGCATTAGCGCCA; ACGGCCACCAGAAGCAGGTGGAGAAGAGTCAGAACAAAGCCAGGCAGTGCAAGGACTCGGCCACCGAGGC; AGAGCGGGTATACAGGCAGAGCATTGCGCAGCTGGAGAAGGTCCGGGCTGAGTGGGAGCAGGAGCACCGG; ACCACCTGTGAGGCCTTTCAGCTGCAAGAGTTTGACCGGCTGACCATTCTCCGCAACGCCCTGTGGGTGC; ACAGCAACCAGCTCTCCATGCAGTGTGTCAAGGATGATGAGCTCTACGAGGAAGTGCGGCTGACGCTGGA; AGGCTGCAGCATAGACGCCGACATCGACAGTTTCATCCAGGCCAAGAGCACGGGCACAGAGCCCCCCGCT; CCGGTGCCCTACCAGAACTATTACGATCGGGAGGTCACCCCGCTGACCAGCAGCCCTGGCATACAGCCGT; CCTGCGGCATGATAAAGAGGTTCTCTGGACTGCTGCACGGAAGTCCCAAGACCACTTCGTTGGCAGCTTC; TGCTGCGTCCACAGAGACCCTGACCCCCACCCCCGAGCGGAATGAGGGTGTCTACACAGCCATCGCAGTG; CAGGAGATACAGGGAAACCCGGCCTCACCAGCCCAGGAGTACCGGGCGCTCTACGATTATACAGCGCAGA; ACCCAGATGAGCTGGACCTGTCCGCGGGAGACATCCTGGAGGTGATCCTGGAAGGGGAGGATGGCTGGTG; GACTGTGGAGAGGAACGGGCAGCGTGGCTTCGTCCCTGGTTCCTACCTGGAGAAGCTTTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003978 unedited; AGGATTTTGTAATACGACTTACTTATAGGGCGGCCGCGAATTCGCACGAGCATGGCCCTT; CATCCGGGGCTGCCCTGGCTGGACAGCCCCCCAGAGCACAGCTGTGTCTGCTCCCAGACT; ATGGGCTCCTTGAGGGCAGGACCTGGTTTGGGTCCCAGAGCAGCCAGGGTTTGATGAATG; ACAGATGCCCTAGACCTGGAAGCTCCTCTCTGGCTCGTGGCTGCCTTCTGAGTGTTGCAG; ACGGCGCCGGCCGGGAAGGGGGGCCTGGGCCAGCCCTGCCAGGACTGGGACGCTGCTGCT; GGCGCCTGGCCCTCCATCAGGCCAGCCTGTGGCAGGAGAGTGAGCTTTGCCGCGGCAGAC; GCCTGAGGATGATGCCCCAGCTGCAGTTCAAAGATGCCTTTTGGTGCAGGGACTTCACAG; CCCACACGGGCTACGAGGTGCTGCTGCAGCGGCTTCTGGATGGCAGGAAGATGTGCAAAG; ACATGGAGGAGCTACTGAGGCAGAGGGCCCAGGCGGAGGAGCGGTACGGGAAGGAGCTGG; TGCAGATCGCACGGAAGGCAGGTGGCCAGACGGAGATCAACTCCCTGAGGGCCTCCTTTG; ACTCCTTGAAGCAGCAAATGGAGAATGTGGGCAGCTCACACATCCAGCTGGCCCTGACCC; TGCGTGAGGAGCTGCGGAGTCTCGAGGAGTTTCGTGAGAGGCAGAAGGAGCAGAGGAAGA; AGTATGAGGCCGTCATGGACCGGGTCCAGAAGAGCAAGCTGTCGCTCTANCAGAAGCCAT; GGAGTCCAAGAAGAATACGAGCANAAGTGCCGGGACGCGGACGACGNCGNAGCAGCCTTC; GAGCGCATTAGCGCCAACGGNCACCAGAAAGCAGTGGAGAAGAGTCAGAACAAGCCCAGC; AGTGCAAGTACTTCGNCCACCGAGCAGAGCGGGTATACAGNCANAGCATGCGCACTTGGA; GAAAGTCCGGCCTGANTGGGAGCAGNAGNACCGTACCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003978 unedited; CGGGCCCCCCCCACCCTCCTTCCCCCNNCGGNAATGGTGAACCCGGCCGCATTTTANGAT; CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAACAAAA; CGCACTCCTTTATTCCCTGGGGAAACCACAGGCTCCCTTTTGGGGCTGGGGGACCCTTGG; TTTTGGGCCCTCACAAGGGGGGACAGTGCTGGGGGCACTGCTGGCTCCACTGGCAGGGCA; GGTCCCAAGGGGCTCCTGGCCCTTCCTCAAAGCTTTTCCAGGTAGGAACCAGGGACCAAA; CCCCCCTGCCCGTTCCTTTCCACAGTCCACCAGCCATTCTCCCCTTTCAGGAACACCTCC; AGGATGTCTCCCGCGGACAGGGCCAACTCATTTGGGTTCTGCCCTGTATAATCGTAAAGC; GCCCGGGACTCCTGGGCTGGGGAGGCCCGGTTTCCCTGTATCTCCTGCACTGGGATGGCT; GGGTAAACACCCTTATTCCCCTCGGGGGTGGGGGGCAGGGTCTTAACAAAAACTGCCAAC; CAAAGGGTCTTGGGACTTCCGGGACAAAACCAAAAAACCTTTTTTTATGCCCCAGGAACG; GTGTTTCCACGGCTGTTTGTCACCGGGGGGAACCTCCCAACGTAAAAATTTTTGGAAGGC; ACCCGGAACCGGGGGCCCTTGGGCCCCCGCCTTTGGGCCCTGAAAAAAATGTCCAATGTC; GGCGGTTTTTTCTTGAAACCTTTTCAAGGTGAAGCCCCACTTTCCCTGAAAAAGTTTTAT; ATCTCTGACCCACATTTATTGAAAAACTTTTTTCTTTGGCCCCCCACAGGGCCTTTCCGG; AAAAAGGGAAACCCGGCCAAACTTTTTGTACTTAGAAGGGCTCCACAGGGGGGTCCGGTG; TTTTTTGTTTCCCATATAAACCGGGACTCTTTTACAAATGGGCAAAGGTTTTGGTCTTGT; AACCCCCTTTTTGCCTGGGGGGCCGAACCCTTCCACACGCCTGGGTTTTTTTCGACACTT; TTTCCCCGGCTCTTGTGGGGCCCTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_003978
• Insert Size: 1830 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003978.2, NP_003969.2
• RefSeq Size: 1781 bp
• RefSeq ORF: 1251 bp
• Locus ID: 9051
• UniProt ID: O43586
• Cytogenetics: 15q24.3
• Domains: FCH, SH3
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• Summary: This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response. [provided by RefSeq, Mar 2016]