FAM107A (NM_001076778) Human Tagged ORF Clone

SKU RG203787

FAM107A (tGFP-tagged) - Human family with sequence similarity 107, member A (FAM107A), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FAM107A
• Synonyms: DRR1; TU3A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203787 representing NM_001076778; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTACTCGGAGATCCAGAGGGAGCGGGCAGACATTGGGGGCCTGATGGCCCGGCCAGAATACAGAGAGT; GGAATCCGGAGCTCATCAAGCCCAAGAAGCTGCTGAACCCCGTGAAGGCCTCTCGGAGTCACCAGGAGCT; CCACCGGGAGCTGCTCATGAACCACAGAAGGGGCCTTGGTGTGGACAGCAAGCCAGAGCTGCAGCGTGTC; CTAGAGCACCGCCGGCGGAACCAGCTCATCAAGAAGAAGAAGGAGGAGCTGGAAGCCAAGCGGCTGCAGT; GCCCCTTTGAGCAGGAGCTGCTGAGACGGCAGCAGAGGCTGAACCAGCTGGAAAAACCACCAGAGAAGGA; AGAGGATCACGCCCCCGAGTTTATTAAAGTCAGGGAAAACCTGCGGAGAATTGCCACACTGACCAGCGAA; GAGAGAGAGCTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203787 representing NM_001076778; Red=Cloning site Green=Tags(s); ; MYSEIQRERADIGGLMARPEYREWNPELIKPKKLLNPVKASRSHQELHRELLMNHRRGLGVDSKPELQRV; LEHRRRNQLIKKKKEELEAKRLQCPFEQELLRRQQRLNQLEKPPEKEEDHAPEFIKVRENLRRIATLTSE; EREL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001076778
• ORF Size: 432 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001076778.3
• RefSeq Size: 3367 bp
• RefSeq ORF: 435 bp
• Locus ID: 11170
• UniProt ID: O95990
• Cytogenetics: 3p14.3-p14.2
• Summary: Stress-inducible actin-binding protein that plays a role in synaptic and cognitive functions by modulating actin filamentous (F-actin) dynamics. Mediates polymerization of globular actin to F-actin. Also binds to, stabilizes and bundles F-actin. Involved in synaptic function by regulating neurite outgrowth in an actin-dependent manner and for the acquisition of hippocampus-dependent cognitive function, such as learning and long-term memory (By similarity). Plays a role in the actin and microtubule cytoskeleton organization; negatively regulates focal adhesion (FA) assembly promoting malignant glial cell migration in an actin-, microtubule- and MAP1A-dependent manner (PubMed:20543869). Also involved in neuroblastoma G1/S phase cell cycle progression and cell proliferation inhibition by stimulating ubiquitination of NF-kappa-B subunit RELA and NF-kappa-B degradation in a COMMD1- and actin-dependent manner (PubMed:10564580, PubMed:28604741). May play a role in tumor development (PubMed:10564580).[UniProtKB/Swiss-Prot Function]