GAPex 5 (GAPVD1) (NM_001282680) Human Tagged ORF Clone

SKU RC240155

GAPVD1 (myc-DDK-tagged) - Human GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GAPex 5
• Synonyms: GAPex-5; GAPEX5; RAP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240155 representing NM_001282680; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGAAACTAGATATTCATACTCTGGCTCATCACCTCAAGCAGGAACGCTTATATGTAAACTCTGAGA; AACAGCTCATTCAGAGGCTCAATGCAGATGTACTTAAGACAGCTGAAAAGTTGTATCGTACAGCATGGAT; TGCGAAGCAACAGAGAATCAATTTGGATCGGCTTATCATAACCAGTGCTGAAGCTTCCCCTGCTGAATGT; TGCCAACATGCCAAAATTTTGGAAGATACACAATTTGTTGATGGGTATAAGCAATTGGGATTTCAGGAGA; CTGCTTATGGAGAATTCTTGAGTCGATTGAGGGAAAATCCTCGTCTTATTGCCTCCTCTTTGGTTGCTGG; AGAGAAACTTAATCAGGAGAACACACAAAGTGTTATTTACACAGTTTTTACCTCCCTGTATGGCAATTGC; ATCATGCAAGAAGATGAAAGCTACCTCCTTCAGGTTTTGCGATACTTGATTGAATTTGAACTTAAAGAAA; GTGACAACCCTAGGCGACTTTTGAGGAGAGGAACTTGTGCCTTCAGCATCTTATTTAAACTTTTTTCTGA; AGGACTGTTTTCTGCCAAACTTTTCCTCACAGCCACTTTACATGAGCCAATTATGCAACTGCTTGTTGAA; GATGAAGATCACCTGGAAACAGATCCAAACAAGCTAATTGAGAGGTTCTCTCCATCTCAGCAGGAAAAAC; TCTTTGGAGAGAAGGGCTCAGATAGATTCAGGCAAAAAGTTCAAGAAATGGTGGAGTCCAATGAAGCAAA; GCTAGTGGCTTTGGTGAACAAATTTATTGGTTATCTCAAACAGAACACATATTGTTTTCCACATAGTTTA; AGGTGGATCGTGTCTCAGATGTACAAAACCCTCTCCTGTGTAGATAGGCTGGAAGTTGGGGAGGTCAGGG; CAATGTGTACTGATCTCCTGTTGGCCTGCTTCATTTGTCCTGCAGTTGTCAATCCAGAACAATATGGAAT; AATTTCCGATGCTCCTATTAATGAAGTAGCACGATTTAATCTGATGCAGGTAGGCCGCCTTTTGCAGCAG; TTAGCAATGACTGGCTCTGAAGAGGGAGATCCCCGAACAAAGAGCAGCCTTGGAAAGTTTGACAAAAGCT; GTGTTGCCGCTTTCCTTGATGTTGTGATTGGGGGCCGTGCAGTGGAGACCCCTCCATTGTCTTCCGTCAA; TCTTCTGGAAGGATTGAGCAGAACTGTGGTTTATATAACCTACAGTCAGCTTATTACTCTGGTGAATTTT; ATGAAGAGTGTGATGTCTGGAGATCAACTGAGAGAAGATAGAATGGCTCTTGACAATTTATTGGCAAACC; TACCCCCGGCCAAGCCAGGAAAAAGTAGCAGTTTAGAAATGACTCCCTACAATACACCTCAGCTATCTCC; AGCAACCACTCCAGCAAATAAAAAGAATCGATTACCTATAGCAACTCGGAGCAGAAGCCGCACCAATATG; CTAATGGACCTACATATGGACCATGAAGGATCATCTCAAGAAACCATCCAGGAGGTGCAACCAGAAGAGG; TGTTGGTCATTTCCTTAGGTACAGGTCCCCAGCTTACTCCAGGGATGATGTCAGAAAATGAGGTCCTAAA; CATGCAGCTTTCGGATGGAGGACAAGGAGATGTCCCTGTTGATGAAAACAAACTCCATGGTAAACCTGAT; AAAACCTTGCGCTTTTCCCTCTGCAGTGATAATCTGGAAGGAATATCTGAAGGTCCTTCAAATCGCTCCA; ATTCAGTGTCCTCCCTAGACCTAGAAGGAGAGTCTGTGTCAGAACTTGGAGCAGGACCTTCTGGCAGTAA; TGGAGTTGAAGCTCTACAGCTGTTAGAACATGAGCAAGCTACAACACAGGATAACCTTGATGATAAGCTA; AGGAAGTTTGAAATTCGTGACATGATGGGATTAACAGATGATAGGGACATATCAGAAACAGTGAGTGAGA; CCTGGAGTACAGACGTCTTGGGAAGTGACTTTGACCCTAATATTGATGAAGATCGCTTGCAAGAAATTGC; AGGTGCTGCAGCAGAGAACATGTTAGGCAGTTTGCTGTGCCTCCCAGGTTCAGGGTCAGTGCTTCTTGAC; CCCTGCACTGGTTCTACCATATCAGAGACAACAAGTGAAGCTTGGAGTGTAGAGGTATTGCCAAGTGACT; CAGAGGCCCCAGACCTAAAGCAGGAGGAGCGTCTGCAAGAACTGGAGAGCTGTTCTGGACTGGGTAGCAC; ATCTGATGATACGGATGTCAGGGAGGTCAGTTCCCGCCCCAGCACACCAGGCCTCAGTGTTGTGTCCGGC; ATAAGTGCAACCTCTGAGGATATTCCCAATAAGATTGAAGACCTGAGATCTGAGTGCAGCTCTGATTTTG; GGGGTAAAGATTCTGTCACTAGTCCAGACATGGATGAAATAACTCACGGTGCCCACCAGCTGACCTCTCC; TCCTTCTCAGTCAGAGTCTCTGCTGGCCATGTTTGATCCACTGTCTTCACATGAAGGGGCTTCTGCTGTG; GTAAGGCCAAAGGTTCACTATGCTAGGCCATCGCATCCACCACCAGATCCCCCAATCCTGGAAGGAGCTG; TGGGAGGAAATGAGGCCAGGTTGCCAAACTTTGGTTCCCATGTTTTAACTCCAGCTGAAATGGAGGCATT; CAAGCAAAGGCATTCTTACCCTGAGAGACTAGTTCGAAGCAGGAGCTCTGATATAGTATCTTCTGTCCGG; AGACCCATGAGTGACCCCAGCTGGAACCGGCGTCCAGGAAATGAAGAGCGAGAACTCCCTCCAGCTGCAG; CCATTGGTGCTACTTCTTTGGTGGCTGCACCTCATTCATCATCTTCATCCCCGAGTAAGGACTCCTCAAG; AGGAGAGACTGAAGAACGCAAAGATAGCGATGATGAGAAATCAGACAGGAACAGACCTTGGTGGAGAAAA; CGTTTTGTTTCAGCCATGCCTAAAGCTCCTATACCATTTAGAAAGAAAGAAAAACAAGAAAAAGACAAAG; ATGATCTGGGGCCTGACAGATTCTCAACACTCACAGATGATCCCAGCCCTAGACTCAGTGCACAAGCTCA; GGTGGCTGAGGATATTCTGGACAAATACAGGAATGCCATTAAACGGACCAGCCCCAGTGATGGAGCAATG; GCAAACTATGAAAGTACAGAGGTTATGGGTGATGGTGAAAGTGCACATGATTCTCCCCGTGACGAAGCAC; TGCAGAACATCTCGGCTGATGATCTCCCAGACTCTGCAAGCCAAGCAGCCCACCCGCAGGATTCAGCTTT; CTCTTACAGAGATGCAAAAAAGAAACTGAGGCTTGCTCTTTGCTCTGCGGACTCTGTTGCCTTCCCAGTG; CTGACCCATTCAACAAGGAATGGTTTACCAGACCACACAGACCCAGAAGACAATGAAATTGTATGCTTCT; TAAAAGTTCAAATAGCTGAAGCAATTAATTTACAAGATAAGAATCTAATGGCTCAACTTCAAGAAACAAT; GCGCTGTGTGTGCCGTTTTGATAATAGGACTTGTAGGAAACTGCTGGCTTCGATTGCTGAGGACTACAGA; AAAAGAGCCCCATATATTGCTTATCTCACTCGTTGTCGACAAGGACTACAGACCACACAGGCTCACCTGG; AAAGGCTATTGCAAAGAGTTTTGCGGGACAAAGAAGTGGCCAATCGATACTTTACCACTGTCTGTGTGAG; ATTACTGCTTGAGAGCAAAGAAAAGAAGATCAGGGAATTCATTCAAGACTTTCAGAAACTCACCGCAGCT; GACGATAAAACTGCTCAGGTAGAAGATTTTCTGCAGTTTCTTTATGGTGCAATGGCCCAGGATGTCATAT; GGCAAAACGCGAGTGAAGAACAGCTTCAAGATGCACAGCTGGCCATTGAGCGAAGCGTGATGAACCGGAT; TTTCAAGCTCGCCTTCTACCCTAATCAAGATGGGGACATACTTCGCGACCAGGTTCTTCATGAACATATC; CAGAGATTGTCTAAAGTAGTGACTGCAAATCACAGAGCTCTTCAGATACCAGAGGTTTATCTTCGAGAAG; CACCATGGCCATCTGCACAATCAGAAATCAGGACAATAAGTGCTTATAAAACCCCCCGGGACAAAGTGCA; GTGCATCCTGAGAATGTGCTCTACGATTATGAACCTCCTGAGCCTGGCCAATGAGGACTCTGTCCCTGGA; GCGGATGACTTTGTTCCTGTGTTGGTGTTTGTGTTGATAAAGGCAAATCCACCCTGTTTGCTGTCTACTG; TGCAGTATATCAGTAGCTTTTATGCTAGCTGTCTGTCTGGAGAGGAGTCCTATTGGTGGATGCAGTTCAC; AGCAGCAGTAGAATTCATTAAAACCATCGATGACCGAAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240155 representing NM_001282680; Red=Cloning site Green=Tags(s); ; MVKLDIHTLAHHLKQERLYVNSEKQLIQRLNADVLKTAEKLYRTAWIAKQQRINLDRLIITSAEASPAEC; CQHAKILEDTQFVDGYKQLGFQETAYGEFLSRLRENPRLIASSLVAGEKLNQENTQSVIYTVFTSLYGNC; IMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVE; DEDHLETDPNKLIERFSPSQQEKLFGEKGSDRFRQKVQEMVESNEAKLVALVNKFIGYLKQNTYCFPHSL; RWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAVVNPEQYGIISDAPINEVARFNLMQVGRLLQQ; LAMTGSEEGDPRTKSSLGKFDKSCVAAFLDVVIGGRAVETPPLSSVNLLEGLSRTVVYITYSQLITLVNF; MKSVMSGDQLREDRMALDNLLANLPPAKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIATRSRSRTNM; LMDLHMDHEGSSQETIQEVQPEEVLVISLGTGPQLTPGMMSENEVLNMQLSDGGQGDVPVDENKLHGKPD; KTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKL; RKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNIDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLD; PCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSG; ISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDEITHGAHQLTSPPSQSESLLAMFDPLSSHEGASAV; VRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHVLTPAEMEAFKQRHSYPERLVRSRSSDIVSSVR; RPMSDPSWNRRPGNEERELPPAAAIGATSLVAAPHSSSSSPSKDSSRGETEERKDSDDEKSDRNRPWWRK; RFVSAMPKAPIPFRKKEKQEKDKDDLGPDRFSTLTDDPSPRLSAQAQVAEDILDKYRNAIKRTSPSDGAM; ANYESTEVMGDGESAHDSPRDEALQNISADDLPDSASQAAHPQDSAFSYRDAKKKLRLALCSADSVAFPV; LTHSTRNGLPDHTDPEDNEIVCFLKVQIAEAINLQDKNLMAQLQETMRCVCRFDNRTCRKLLASIAEDYR; KRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAA; DDKTAQVEDFLQFLYGAMAQDVIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHI; QRLSKVVTANHRALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPG; ADDFVPVLVFVLIKANPPCLLSTVQYISSFYASCLSGEESYWWMQFTAAVEFIKTIDDRK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282680
• ORF Size: 4380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001282680.2
• RefSeq Size: 7029 bp
• RefSeq ORF: 4383 bp
• Locus ID: 26130
• UniProt ID: Q14C86
• Cytogenetics: 9q33.3
• MW: 163.3 kDa
• Summary: Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.[UniProtKB/Swiss-Prot Function]