RBM16 (SCAF8) (NM_001286189) Human Tagged ORF Clone

SKU RC240113

SCAF8 (myc-DDK-tagged) - Human SR-related CTD-associated factor 8 (SCAF8), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: RBM16
• Synonyms: RBM16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC240113 representing NM_001286189; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCGCAGCCGCTGCTGCCAGCGCTTCCTCCTCTGTCTTCGCCGAGCGGGGCTGGTTCCTGCGGCCCGA; GCGGCGGGGAGTGCAGTGGCCGCCGCCTCTTCCGCCGCCGGGCTCGGGGCCTCCGCAGCGACAACATGGA; GGCCGTGAAGACCTTCAATAGCGAGGATTGTGCCTCTACTCCTGCTTGTACAGACTGCTTTTCCAGCAGC; ATGGACACAAGAAGCATATTGTATTCCCTGAATGACTATAAACCACCCATTTCGAAAGCGAAAATGACCC; AAATTACTAAGGCAGCCATCAAAGCTATTAAGTTCTATAAACATGTGGTACAGAGTGTTGAGAAGTTTAT; TCAGAAATGTAAACCAGAATACAAAGTACCTGGACTTTATGTTATTGACTCCATTGTGCGACAATCCCGA; CATCAGTTTGGTCAAGAAAAGGATGTGTTTGCACCCAGATTTAGTAATAACATCATTAGCACTTTCCAGA; ATTTATATCGTTGCCCTGGGGATGACAAGAGTAAAATAGTGAGAGTACTAAACTTATGGCAGAAGAATAA; TGTATTTAAGAGTGAGATTATTCAGCCCCTTTTGGATATGGCAGCCGGGATTCCGCCTCCAGTTGTCACA; CCTGTTTTGGCCAGCACTACCACTGCTATGAGCAATACTCCAGGAACTCCTGTGACACCTGTTACTCCGG; CCAATGTGGTCCAAGGCTTACCTGATCCGTGGGTATCTCAGATAACAAATACAGATACACTTGCGGCTGT; AGCTCAGATCTTGCAAAGTCCTCAAGGCCAGCAGCTTCAACAATTAATACAAACCTTACAGATACAACAA; CAGAAGCCCCAGCCTTCCATTCTGCAGGCCCTAGATGCTGGTCTTGTTGTTCAGTTGCAAGCTCTTACGG; CACAACTTACAGCTGCAGCTGCAGCTGCCAACACTCTTACTCCCTTAGAACAGGGAGTCTCCTTTAACAA; GAAGTTGATGGATAGGTTTGATTTTGGGGAAGACTCTGAGCATAGTGAAGAACCCAAAAAGGAAATTCCA; GCTTCACAACTTTCTCACGTTTCAGAATCTGTGAACAATTCCATTTTTCATCAGATAGCAGAACAACTAC; AACAGCAAAACCTAGAACATCTCAGACAGCAGCTCTTGGAGCAGCAACAGCCTCAAAAGGCCACTCCTCA; GGATAGTCAGGAAGGAACCTTTGGGTCAGAGCATTCAGCGTCACCATCACAAGGGAGTAGTCAGCAGCAT; TTTCTTGAACCTGAAGTCAATTTGGATGATTCCATAGATATTCAGCAACAGGATATGGATATAGATGAAG; GGCAAGATGGAGTGGAAGAGGAGGTCTTTGAACAAGAAGCTAAGAAAGTGGCGGTTCGCTCAAGATCAAG; AACACATTCACGATCTCGTTCAAGATCACCAAGAAAACGAAGGTCTAGGTCACGGTCTGGCTCTAGAAAG; CGTAAACACAGAAAGCGATCACGCTCCCGCTCAAGAGAAAGAAAGAGGAAATCATCACGGTCGTATTCAA; GTGAAAGGAGAGCCAGAGAAAGGGAGAAAGAACGACAGAAAAAGGGATTACCTCCAATTAGATCTAAAAC; ACTAAGTGTATGTAGTACTACTCTCTGGGTTGGGCAAGTGGACAAGAAGGCAACACAGCAAGACTTAACC; AACCTGTTTGAAGAGTTTGGACAGATTGAATCCATTAATATGATTCCTCCCCGGGGCTGTGCTTATGTCT; GCATGGTTCATCGACAAGATGCATTTCGAGCTCTTCAGAAACTCAGTTCTGGATCATATAAAATTGGGTC; CAAGGTCATTAAGATCGCTTGGGCTTTAAACAAAGGTGTAAAAACAGAATACAAACAATTCTGGGATGTG; GATCTTGGAGTTACATATATACCATGGGAAAAAGTTAAAGTGGATGACTTGGAAGGTTTTGCAGAAGGAG; GCATGATTGATCAGGAGACTGTAAATACTGAGTGGGAAACTGTGAAAAGCTCAGAACCTGTTAAAGAGAC; GGTCCAGACAACTCAGAGCCCAACTCCAGTTGAAAAGGAGACAGTGGTCACAACCCAGGCAGAGGTTTTC; CCTCCTCCTGTTGCTATGTTGCAGATTCCAGTGGCGCCAGCCGTGCCTACAGTTAGTTTAGTCCCACCAG; CATTTCCTGTGTCGATGCCGGTTCCTCCTCCTGGATTCAGTCCAATCCCTCCACCTCCTTTTTTAAGAGC; AAGTTTTAACCCTTCACAACCACCACCTGGTTTCATGCCGCCTCCAGTTCCCCCACCTGTTGTGCCACCC; CCTACGATTCCACCAGTAGTACCAACATCTTTAGTGCAGCCGTCATTATCCATGACACCGGAAACTGTGA; AAGATGTTGGATTTGGTAGCCTTGTTATACCAGGCGGTTCTGTTGCCAGCAATCTTGCTACTTCCGCTCT; GCCAGCTGGAAATGTTTTTAATGCTCCAACTAAACAGGCAGAGCCTGAAGAAAAAGTACCTCATCTTATA; GACCACCAGATTTCTTCTGGTGAAAACACCAGATCAGTGATTCCAAATGATATTTCAAGTAATGCTGCAA; TTTTAGGAGGACAGCCGCCAAATGTGACAAGCAATTCTGGAATTCTGGGAGTCCAAAGACCAAATGTATC; AAGTAATTCTGAAATTCTTGGGGTCCGGCCATCTAATGTTTCCAGTAGTTCTGGGATTATTGCAGCCCAA; CCACCAAATATTCTAAATAACTCTGGAATATTGGGAATACAGCCACCCAGTGTGTCAAATAGTTCTGGAC; TTTTGGGAGTGCTACCCCCAAATATACCTAACAATTCTGGACTTGTAGGAGTACAGCCACCAAATGTTCC; AAATACTCCTGGACTTCTGGGAACACAGCCACCAGCTGGACCTCAAAACTTACCCCCTTTAAGTATCCCT; AATCAAAGGATGCCCACAATGCCAATGTTAGACATTCGTCCGGGACTAATACCACAGGCACCTGGGCCAA; GATTCCCTTTAATACAGCCTGGAATTCCACCCCAACGGGGAATCCCACCCCCATCGGTACTTGATTCAGC; TCTTCATCCACCACCCCGTGGACCTTTTCCTCCAGGAGATATTTTTAGTCAACCAGAAAGACCTTTTTTA; GCTCCTGGAAGACAAAGCGTAGACAATGTTACTAACCCAGAAAAAAGGATACCACTTGGGAATGATAACA; TTCAACAGGAAGGAGATAGAGATTACCGGTTTCCTCCTATAGAAACCAGGGAAAGCATTAGTAGACCTCC; CCCTGTGGATGTTAGAGATGTGGTTGGGCGGCCTATAGATCCAAGAGAAGGTCCTGGACGGCCTCCACTA; GATGGTAGGGATCATTTTGGAAGACCTCCTGTAGATATAAGAGAGAATCTTGTGAGGCCAGGTATAGATC; ATCTTGGTCGAAGAGACCACTTTGGCTTTAATCCAGAGAAGCCCTGGGGGCATAGAGGAGATTTTGATGA; GAGAGAGCATCGGGTTCTACCGGTCTATGGTGGTCCAAAAGGCTTACATGAAGAAAGAGGTAGATTTCGG; TCTGGAAACTATCGATTTGATCCTAGAAGTGGTCCTTGGAACCGAGGATTTGGACAAGAAGTTCACAGAG; ATTTTGATGACCGCAGAAGACCCTGGGAGAGGCAAAGGGATAGGGATGACAGAGATTTTGATTTCTGCAG; AGAAATGAATGGAAATCGTCTTGGACGAGACAGAATTCAAAACACTTGGGTTCCCCCTCCTCATGCTCGG; GTTTTTGATTATTTTGAAGGGGCCACTTCTCAACGAAAAGGTGATAATGTGCCTCAGGTTAATGGTGAAA; ATACAGAGAGACATGCTCAGCCACCACCTATACCAGTACAGAATGATCCTGAACTTTATGAAAAACTGAC; ATCTTCAAATGAAATAAACAAGGAGAAGAGTGACACAGTTGCTGATATAGAAAGTGAACCAGTGGTAGAA; AGCACAGAAACTGAGGGGACA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC240113 representing NM_001286189; Red=Cloning site Green=Tags(s); ; MPQPLLPALPPLSSPSGAGSCGPSGGECSGRRLFRRRARGLRSDNMEAVKTFNSEDCASTPACTDCFSSS; MDTRSILYSLNDYKPPISKAKMTQITKAAIKAIKFYKHVVQSVEKFIQKCKPEYKVPGLYVIDSIVRQSR; HQFGQEKDVFAPRFSNNIISTFQNLYRCPGDDKSKIVRVLNLWQKNNVFKSEIIQPLLDMAAGIPPPVVT; PVLASTTTAMSNTPGTPVTPVTPANVVQGLPDPWVSQITNTDTLAAVAQILQSPQGQQLQQLIQTLQIQQ; QKPQPSILQALDAGLVVQLQALTAQLTAAAAAANTLTPLEQGVSFNKKLMDRFDFGEDSEHSEEPKKEIP; ASQLSHVSESVNNSIFHQIAEQLQQQNLEHLRQQLLEQQQPQKATPQDSQEGTFGSEHSASPSQGSSQQH; FLEPEVNLDDSIDIQQQDMDIDEGQDGVEEEVFEQEAKKVAVRSRSRTHSRSRSRSPRKRRSRSRSGSRK; RKHRKRSRSRSRERKRKSSRSYSSERRAREREKERQKKGLPPIRSKTLSVCSTTLWVGQVDKKATQQDLT; NLFEEFGQIESINMIPPRGCAYVCMVHRQDAFRALQKLSSGSYKIGSKVIKIAWALNKGVKTEYKQFWDV; DLGVTYIPWEKVKVDDLEGFAEGGMIDQETVNTEWETVKSSEPVKETVQTTQSPTPVEKETVVTTQAEVF; PPPVAMLQIPVAPAVPTVSLVPPAFPVSMPVPPPGFSPIPPPPFLRASFNPSQPPPGFMPPPVPPPVVPP; PTIPPVVPTSLVQPSLSMTPETVKDVGFGSLVIPGGSVASNLATSALPAGNVFNAPTKQAEPEEKVPHLI; DHQISSGENTRSVIPNDISSNAAILGGQPPNVTSNSGILGVQRPNVSSNSEILGVRPSNVSSSSGIIAAQ; PPNILNNSGILGIQPPSVSNSSGLLGVLPPNIPNNSGLVGVQPPNVPNTPGLLGTQPPAGPQNLPPLSIP; NQRMPTMPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFL; APGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPL; DGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFR; SGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHAR; VFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVE; STETEGT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286189
• ORF Size: 4011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001286189.1, NP_001273118.1
• RefSeq Size: 4908 bp
• RefSeq ORF: 4014 bp
• Locus ID: 22828
• UniProt ID: Q9UPN6
• Cytogenetics: 6q25.2
• MW: 147.8 kDa
• Summary: Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839).[UniProtKB/Swiss-Prot Function]