Pescadillo (PES1) (NM_001282327) Human Tagged ORF Clone

SKU RC238300

PES1 (myc-DDK-tagged) - Human pescadillo ribosomal biogenesis factor 1 (PES1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Pescadillo
• Synonyms: NOP7; PES
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238300 representing NM_001282327; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTGCTTCCTGTTTTCCACCTTCCCGCGGACTGGCAAGTGCCACGTGCAGACCATTCAGCTGTGCCGCC; GGCTCACTGTGGAGTTCATGCACTACATTATCGCTGCCCGTGCCCTGCGCAAGGTCTTCCTGTCCATCAA; AGGCATTTACTACCAGGCCGAGGTACTGGGGCAGCCCATCGTGTGGATCACTCCCTATGCCTTCTCCCAT; GACCACCCGACAGACGTGGACTACAGGGTCATGGCCACCTTCACCGAGTTCTACACCACGCTGCTGGGCT; TTGTCAACTTCCGCCTTTACCAGTTGCTCAACCTCCACTATCCCCCGAAGCTCGAGGGTCAGGCCCAAGC; AGAGGCAAAGGCCGGTGAGGGCACCTACGCGTTGGACTCCGAGAGTTGTATGGAGAAACTGGCAGCCCTC; AGTGCCAGCCTGGCCCGCGTGGTGGTGCCTGCCACAGAGGAGGAGGCCGAGGTGGATGAGTTTCCCACCG; ATGGGGAGATGTCAGCGCAGGAGGAAGACCGCAGGAAGGAGCTGGAGGCGCAGGAGAAGCACAAGAAGCT; TTTTGAGGGCCTGAAGTTCTTCCTGAACCGAGAGGTGCCCCGTGAGGCCCTGGCCTTCATCATCAGGAGT; TTTGGTGGGGAAGTGTCCTGGGACAAATCTTTGTGCATTGGGGCCACCTATGACGTCACAGACTCCCGCA; TCACCCATCAGATTGTCGACCGGCCTGGGCAGCAGACCTCAGTCATTGGCAGGTGCTACGTGCAGCCCCA; GTGGGTGTTTGACTCAGTGAACGCCAGGCTCCTTCTCCCCGTGGCAGAGTACTTCTCTGGGGTGCAGCTG; CCCCCACACCTTTCACCCTTTGTGACCGAGAAGGAAGGAGATTACGTTCCACCTGAGAAGCTGAAGCTGC; TGGCTCTGCAGCGGGGAGAGGACCCAGGAAACCTGAATGAGTCAGAAGAGGAGGAGGAAGAGGACGACAA; CAACGAAGGTGATGGTGATGAAGAGGGAGAAAATGAGGAGGAGGAGGAAGATGCAGAGGCTGGTTCAGAA; AAGGAGGAAGAGGCCCGGCTGGCAGCCCTGGAAGAGCAGAGGATGGAGGGGAAGAAGCCCAGGGTGATGG; CAGGCACCTTGAAGCTGGAGGATAAGCAGCGGCTGGCCCAGGAGGAGGAGAGTGAGGCCAAGCGCCTGGC; CATTATGATGATGAAGAAGCGGGAGAAGTACCTGTACCAGAAGATCATGTTTGGCAAGAGGCGAAAAATC; CGAGAGGCCAACAAGCTGGCGGAGAAGCGGAAAGCCCACGATGAGGCGGTGAGGTCTGAGAAGAAGGCCA; AGAAGGCAAGGCCGGAG; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238300 representing NM_001282327; Red=Cloning site Green=Tags(s); ; MCFLFSTFPRTGKCHVQTIQLCRRLTVEFMHYIIAARALRKVFLSIKGIYYQAEVLGQPIVWITPYAFSH; DHPTDVDYRVMATFTEFYTTLLGFVNFRLYQLLNLHYPPKLEGQAQAEAKAGEGTYALDSESCMEKLAAL; SASLARVVVPATEEEAEVDEFPTDGEMSAQEEDRRKELEAQEKHKKLFEGLKFFLNREVPREALAFIIRS; FGGEVSWDKSLCIGATYDVTDSRITHQIVDRPGQQTSVIGRCYVQPQWVFDSVNARLLLPVAEYFSGVQL; PPHLSPFVTEKEGDYVPPEKLKLLALQRGEDPGNLNESEEEEEEDDNNEGDGDEEGENEEEEEDAEAGSE; KEEEARLAALEEQRMEGKKPRVMAGTLKLEDKQRLAQEEESEAKRLAIMMMKKREKYLYQKIMFGKRRKI; REANKLAEKRKAHDEAVRSEKKAKKARPE; ; SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282327
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001282327.1, NP_001269256.1
• RefSeq Size: 2710 bp
• RefSeq ORF: 1350 bp
• Locus ID: 23481
• Cytogenetics: 22q12.2
• MW: 51.9 kDa
• Summary: This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9. [provided by RefSeq, Aug 2011]