CEBP Alpha (CEBPA) (NM_001287424) Human Tagged ORF Clone

SKU RC237942

CEBPA (myc-DDK-tagged) - Human CCAAT/enhancer binding protein (C/EBP), alpha (CEBPA), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CEBP Alpha
• Synonyms: C/EBP-alpha; CEBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237942 representing NM_001287424; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; GTGCGCGGGCGCGGGCGAGCAGGGTCTCCGGGTGGGCGGCGGCGACGCCCCGCGCAGGCTGGAGGCCGCC; GAGGCTCGCCATGCCGGGAGAACTCTAACTCCCCCATGGAGTCGGCCGACTTCTACGAGGCGGAGCCGCG; GCCCCCGATGAGCAGCCACCTGCAGAGCCCCCCGCACGCGCCCAGCAGCGCCGCCTTCGGCTTTCCCCGG; GGCGCGGGCCCCGCGCAGCCTCCCGCCCCACCTGCCGCCCCGGAGCCGCTGGGCGGCATCTGCGAGCACG; AGACGTCCATCGACATCAGCGCCTACATCGACCCGGCCGCCTTCAACGACGAGTTCCTGGCCGACCTGTT; CCAGCACAGCCGGCAGCAGGAGAAGGCCAAGGCGGCCGTGGGCCCCACGGGCGGCGGCGGCGGCGGCGAC; TTTGACTACCCGGGCGCGCCCGCGGGCCCCGGCGGCGCCGTCATGCCCGGGGGAGCGCACGGGCCCCCGC; CCGGCTACGGCTGCGCGGCCGCCGGCTACCTGGACGGCAGGCTGGAGCCCCTGTACGAGCGCGTCGGGGC; GCCGGCGCTGCGGCCGCTGGTGATCAAGCAGGAGCCCCGCGAGGAGGATGAAGCCAAGCAGCTGGCGCTG; GCCGGCCTCTTCCCTTACCAGCCGCCGCCGCCGCCGCCGCCCTCGCACCCGCACCCGCACCCGCCGCCCG; CGCACCTGGCCGCCCCGCACCTGCAGTTCCAGATCGCGCACTGCGGCCAGACCACCATGCACCTGCAGCC; CGGTCACCCCACGCCGCCGCCCACGCCCGTGCCCAGCCCGCACCCCGCGCCCGCGCTCGGTGCCGCCGGC; CTGCCGGGCCCTGGCAGCGCGCTCAAGGGGCTGGGCGCCGCGCACCCCGACCTCCGCGCGAGTGGCGGCA; GCGGCGCGGGCAAGGCCAAGAAGTCGGTGGACAAGAACAGCAACGAGTACCGGGTGCGGCGCGAGCGCAA; CAACATCGCGGTGCGCAAGAGCCGCGACAAGGCCAAGCAGCGCAACGTGGAGACGCAGCAGAAGGTGCTG; GAGCTGACCAGTGACAATGACCGCCTGCGCAAGCGGGTGGAACAGCTGAGCCGCGAACTGGACACGCTGC; GGGGCATCTTCCGCCAGCTGCCAGAGAGCTCCTTGGTCAAGGCCATGGGCAACTGCGCG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237942 representing NM_001287424; Red=Cloning site Green=Tags(s); ; VRGRGRAGSPGGRRRRPAQAGGRRGSPCRENSNSPMESADFYEAEPRPPMSSHLQSPPHAPSSAAFGFPR; GAGPAQPPAPPAAPEPLGGICEHETSIDISAYIDPAAFNDEFLADLFQHSRQQEKAKAAVGPTGGGGGGD; FDYPGAPAGPGGAVMPGGAHGPPPGYGCAAAGYLDGRLEPLYERVGAPALRPLVIKQEPREEDEAKQLAL; AGLFPYQPPPPPPPSHPHPHPPPAHLAAPHLQFQIAHCGQTTMHLQPGHPTPPPTPVPSPHPAPALGAAG; LPGPGSALKGLGAAHPDLRASGGSGAGKAKKSVDKNSNEYRVRRERNNIAVRKSRDKAKQRNVETQQKVL; ELTSDNDRLRKRVEQLSRELDTLRGIFRQLPESSLVKAMGNCA; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287424
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001287424.2
• RefSeq Size: 2631 bp
• RefSeq ORF: 1182 bp
• Locus ID: 1050
• UniProt ID: P49715
• Cytogenetics: 19q13.11
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• MW: 41.7 kDa
• Summary: This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]