Liprin alpha 2 (PPFIA2) (NM_001220473) Human Tagged ORF Clone

SKU RC235242

PPFIA2 (Myc-DDK tagged) - Homo sapiens protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 (PPFIA2), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Liprin alpha 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235242 representing NM_001220473; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATGTGTGAAGTGATGCCCACGATTAATGAGGACACCCCAATGAGCCAAAGGGGGTCCCAAAGCAGTG; GCTCGGACTCAGACTCCCATTTTGAGCAGCTGATGGTGAATATGCTAGATGAAAGGGATCGTCTTCTAGA; CACCCTTCGGGAGACCCAGGAAAGCCTCTCACTTGCCCAGCAAAGACTTCAGGATGTCATCTATGACCGA; GACTCACTCCAGAGACAGCTCAATTCAGCCCTGCCACAGGATATCGAATCCCTAACAGGAGGGCTGGCTG; GTTCTAAGGGGGCTGATCCACCGGAATTTGCTGCACTGACAAAAGAATTAAATGCCTGCAGGGAACAACT; TCTAGAAAAGGAAGAAGAAATCTCTGAACTTAAAGCTGAAAGAAACAACACAAGACTATTACTGGAGCAT; TTGGAGTGCCTTGTGTCACGACATGAAAGATCACTAAGAATGACGGTGGTAAAACGGCAAGCCCAGTCTC; CCTCAGGAGTATCCAGTGAAGTTGAAGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAGGCCTT; GGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAAGAACTAGCT; GCTGCTAATCAGGAGATTGTTGCCTTGCGTGAACAAAATGTTCATATACAAAGAAAAATGGCATCAAGCG; AGGGATCCACAGAGTCAGAACATCTTGAAGGGATGGAACCTGGACAGAAAGTCCATGAGAAGCGTTTGTC; CAATGGTTCTATAGACTCAACCGATGAAACTAGTCAAATAGTTGAACTACAAGAATTGCTTGAAAAGCAA; AACTATGAAATGGCCCAGATGAAAGAACGTTTAGCAGCCCTTTCTTCCCGAGTGGGAGAGGTGGAACAGG; AAGCAGAGACAGCAAGAAAGGATCTCATTAAAACAGAAGAAATGAACACCAAGTATCAAAGGGACATTAG; GGAGGCCATGGCACAAAAGGAAGATATGGAAGAAAGAATTACAACCCTTGAAAAGCGTTACCTCAGTGCT; CAGAGAGAATCTACCTCCATACATGACATGAATGATAAACTAGAAAATGAGTTAGCAAATAAAGAAGCTA; TCCTGCGGCAGATGGAAGAGAAAAACAGACAGTTACAAGAACGTCTTGAGCTAGCTGAACAAAAGTTGCA; GCAGACCATGAGAAAGGCTGAAACCTTGCCTGAAGTAGAGGCTGAACTGGCTCAGAGAATTGCAGCCCTA; ACCAAGGCTGAAGAGAGACATGGAAATATTGAAGAACGTATGAGACATTTAGAGGGTCAACTTGAAGAGA; AGAATCAAGAACTTCAAAGAGCTAGGCAAAGAGAGAAAATGAATGAGGAGCATAACAAGAGATTATCGGA; TACGGTTGATAGACTTCTGACTGAATCCAATGAACGCCTACAACTACACTTAAAGGAAAGAATGGCTGCT; CTAGAAGAAAAGAATGTTTTAATTCAAGAATCAGAAACTTTCAGAAAGAATCTTGAAGAATCTTTACATG; ATAAGGAAAGATTAGCAGAAGAAATTGAAAAGCTGAGATCTGAACTTGACCAATTGAAAATGAGAACTGG; CTCTTTAATTGAACCCACAATACCAAGAACTCATCTAGACACCTCAGCTGAGTTGCGGTACTCAGTGGGA; TCCCTAGTGGACAGCCAGTCTGATTACAGAACAACTAAAGTAATAAGAAGACCAAGGAGAGGCCGCATGG; GTGTGCGAAGAGATGAGCCAAAGGTGAAATCTCTTGGGGATCACGAGTGGAATAGAACTCAACAGATTGG; AGTACTAAGCAGCCACCCTTTTGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACA; ATTTTTAGCTCAATGGATCTTCTCTCTCCAAGTGGTCATTCCGATGCCCAGACGCTAGCCATGATGCTTC; AGGAACAATTGGATGCCATCAACAAAGAAATCAGGCTAATTCAGGAAGAAAAAGAATCTACAGAGTTGCG; TGCTGAAGAAATTGAAAATAGAGTGGCTAGTGTGAGCCTCGAAGGCCTGAATTTGGCAAGGGTCCACCCA; GGTACCTCCATTACTGCCTCTGTTACAGCTTCATCGCTGGCCAGTTCATCTCCCCCCAGTGGACACTCAA; CTCCAAAGCTCACCCCTCGAAGCCCTGCCAGGGAAATGGATCGGATGGGAGTCATGACACTGCCAAGTGA; TCTGAGGAAACATCGGAGAAAGATTGCAGTTGTGGAAGAAGATGGTCGAGAGGACAAAGCAACAATTAAA; TGTGAAACTTCTCCTCCTCCTACCCCTAGAGCCCTCAGAATGACTCACACTCTCCCTTCTTCCTACCACA; ATGATGCTCGAAGTAGTTTATCTGTCTCTCTTGAGCCAGAAAGCCTCGGGCTTGGTAGTGCCAACAGCAG; CCAAGACTCTCTTCACAAAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATAGGACGTTTGTTTGGTAAA; AAAGAAAAAGCTCGACTTGGGCAGCTCCGAGGCTTTATGGAGACTGAAGCTGCAGCTCAGGAGTCCCTGG; GGTTAGGCAAACTCGGAACTCAAGCTGAGAAGGATCGAAGACTAAAGAAAAAGCATGAACTTCTTGAAGA; AGCTCGGAGAAAGGGATTACCTTTTGCCCAGTGGGATGGGCCAACTGTGGTCGCATGGCTAGAGCTTTGG; TTGGGAATGCCTGCGTGGTACGTGGCAGCCTGCCGAGCCAACGTGAAGAGTGGTGCCATCATGTCTGCTT; TATCTGACACTGAGATCCAGAGAGAAATTGGAATCAGCAATCCACTGCATCGCTTAAAACTTCGATTAGC; AATCCAGGAGATGGTTTCCCTAACAAGTCCTTCAGCTCCTCCAACATCTCGAACTCCTTCAGGCAACGTT; TGGGTGACTCATGAAGAAATGGAAAATCTTGCAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCT; GGGCCCAGTGTCCGGTTTTTCTACAGACCCTGGCTTATGGAGATATGAATCATGAGTGGATTGGAAATGA; ATGGCTTCCCAGCTTGGGGTTACCTCAGTACAGAAGTTACTTTATGGAATGCTTGGTAGATGCAAGAATG; TTAGATCACCTAACAAAAAAAGATCTCCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGAACAAGTT; TACAATATGGAATTATGTGCTTAAAGAGGTTGAATTATGACAGAAAAGAACTAGAAAGAAGACGGGAAGC; AAGCCAACATGAAATAAAAGACGTGTTGGTGTGGAGCAATGACCGAGTTATTCGCTGGATACAAGCAATT; GGACTTCGAGAATATGCAAATAATATACTTGAGAGCGGTGTGCATGGCTCACTTATAGCCCTGGATGAAA; ACTTTGACTACAGCAGCTTAGCTTTATTATTACAGATTCCAACACAGAACACCCAGGCAAGGCAGATTCT; TGAAAGAGAATACAATAACCTCTTGGCCCTGGGAACTGAAAGGCGACTGGATGAAAGTGATGACAAGAAC; TTCAGACGTGGATCAACCTGGAGAAGGCAGTTTCCTCCTCGTGAAGTACATGGAATCAGCATGATGCCTG; GGTCCTCAGAAACATTACCAGCTGGATTTAGGTTAACCACAACCTCTGGGCAGTCAAGAAAAATGACAAC; AGATGATGGCGTCTTTTCAGTCTACTCTACC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235242 representing NM_001220473; Red=Cloning site Green=Tags(s); ; MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLSLAQQRLQDVIYDR; DSLQRQLNSALPQDIESLTGGLAGSKGADPPEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEH; LECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELA; AANQEIVALREQNVHIQRKMASSEGSTESEHLEGMEPGQKVHEKRLSNGSIDSTDETSQIVELQELLEKQ; NYEMAQMKERLAALSSRVGEVEQEAETARKDLIKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSA; QRESTSIHDMNDKLENELANKEAILRQMEEKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAAL; TKAEERHGNIEERMRHLEGQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAA; LEEKNVLIQESETFRKNLEESLHDKERLAEEIEKLRSELDQLKMRTGSLIEPTIPRTHLDTSAELRYSVG; SLVDSQSDYRTTKVIRRPRRGRMGVRRDEPKVKSLGDHEWNRTQQIGVLSSHPFESDTEMSDIDDDDRET; IFSSMDLLSPSGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARVHP; GTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIK; CETSPPPTPRALRMTHTLPSSYHNDARSSLSVSLEPESLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGK; KEKARLGQLRGFMETEAAAQESLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELW; LGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNV; WVTHEEMENLAAPAKTKESEEGSWAQCPVFLQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARM; LDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKRLNYDRKELERRREASQHEIKDVLVWSNDRVIRWIQAI; GLREYANNILESGVHGSLIALDENFDYSSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKN; FRRGSTWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGQSRKMTTDDGVFSVYST; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001220473
• ORF Size: 3741 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001220473.3
• RefSeq Size: 5699 bp
• RefSeq ORF: 3744 bp
• Locus ID: 8499
• Cytogenetics: 12q21.31
• Protein Families: Druggable Genome
• MW: 142.6 kDa
• Summary: The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein has been shown to bind the calcium/calmodulin-dependent serine protein kinase (MAGUK family) protein (also known as CASK) and proposed to regulate higher-order brain functions in mammals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]