NrCAM (NM_001193584) Human Tagged ORF Clone

SKU RC230893

NRCAM (Myc-DDK-tagged)-Human neuronal cell adhesion molecule (NRCAM), transcript variant 6

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: NRCAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230893 representing NM_001193584; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCAGCTTAAAATAATGCCGAAAAAGAAGCGCTTATCTGCGGGCAGAGTGCCCCTGATTCTCTTCCTGT; GCCAGATGATTAGTGCACTGGAAGTACCTCTTGATCCAAAACTTCTTGAAGACTTGGTACAGCCTCCAAC; CATCACCCAACAGTCTCCAAAAGATTACATTATTGACCCTCGGGAGAATATTGTAATCCAGTGTGAAGCC; AAAGGGAAACCGCCCCCAAGCTTTTCCTGGACCCGTAATGGGACTCATTTTGACATCGATAAAGACCCTC; TGGTCACCATGAAGCCTGGCACAGGAACGCTCATAATTAACATCATGAGCGAAGGGAAAGCTGAGACCTA; TGAAGGAGTCTATCAGTGTACAGCAAGGAACGAACGCGGAGCTGCAGTTTCTAATAACATTGTTGTCCGC; CCATCCAGATCACCATTGTGGACCAAAGAAAAACTTGAACCAATCACACTTCAAAGTGGTCAGTCTTTAG; TACTTCCCTGCAGACCCCCAATTGGATTACCACCACCTATAATATTTTGGATGGATAATTCCTTTCAAAG; ACTTCCACAAAGTGAGAGAGTTTCTCAAGGTTTGAATGGGGACCTTTATTTTTCCAATGTCCTCCCAGAG; GACACCCGCGAAGACTATATCTGTTATGCTAGATTTAATCATACTCAAACCATACAGCAGAAGCAACCTA; TTTCTGTGAAGGTGATTTCAGCTAAATCAAGTAGAGAGAGGCCACCAACATTTTTAACTCCAGAAGGCAA; TGCAAGTAACAAAGAGGAATTAAGAGGAAATGTGCTTTCACTGGAGTGCATTGCAGAAGGACTGCCTACC; CCAATTATTTACTGGGCAAAGGAAGATGGAATGCTACCCAAAAACAGGACAGTTTATAAGAACTTTGAGA; AAACCTTGCAGATCATTCATGTTTCAGAAGCAGACTCTGGAAATTACCAATGTATAGCAAAAAATGCATT; AGGAGCCATCCACCATACCATTTCTGTTAGAGTTAAAGCGGCTCCATACTGGATCACAGCCCCTCAAAAT; CTTGTGCTGTCCCCAGGAGAGGATGGGACCTTGATCTGCAGAGCTAATGGCAACCCCAAACCCAGAATTA; GCTGGTTAACAAATGGAGTCCCAATAGAAATTGCCCCTGATGACCCCAGCAGAAAAATAGATGGCGATAC; CATTATTTTTTCAAATGTTCAAGAAAGATCAAGTGCAGTATATCAGTGCAATGCCTCTAATGAATATGGA; TATTTACTGGCAAACGCATTTGTAAATGTGCTGGCTGAGCCACCACGAATCCTCACACCTGCAAACACAC; TCTACCAGGTCATTGCAAACAGGCCTGCTTTACTAGACTGTGCCTTCTTTGGGTCTCCTCTCCCAACCAT; CGAGTGGTTTAAAGGAGCTAAAGGAAGTGCTCTTCATGAAGATATTTATGTTTTACATGAAAATGGAACT; TTGGAAATTCCTGTGGCCCAAAAGGACAGTACAGGAACTTATACGTGTGTTGCAAGGAATAAATTAGGGA; TGGCAAAGAATGAAGTTCACTTAGAAATCAAAGATGCTACATGGATCGTTAAACAGCCCGAATATGCAGT; TGTGCAAAGAGGGAGCATGGTGTCCTTTGAATGCAAAGTGAAACATGATCACACCTTATCCCTCACTGTC; CTGTGGCTGAAGGACAACAGGGAACTGCCCAGTGATGAAAGGTTCACTGTTGACAAGGATCATCTAGTGG; TAGCTGATGTCAGTGACGATGACAGCGGGACCTACACGTGTGTGGCCAACACCACTCTGGACAGCGTCTC; CGCCAGCGCTGTGCTTAGCGTTGTTGCTCCTACTCCAACTCCAGCTCCCGTTTACGATGTCCCAAATCCT; CCCTTTGACTTAGAACTGACAGATCAACTTGACAAAAGTGTTCAGCTGTCATGGACCCCAGGCGATGACA; ACAATAGCCCCATTACAAAATTCATCATCGAATATGAAGATGCAATGCACAAGCCAGGGCTGTGGCACCA; CCAAACTGAAGTTTCTGGAACACAGACCACAGCCCAGCTGAAGCTGTCTCCTTACGTGAACTACTCCTTC; CGCGTGATGGCAGTGAACAGCATTGGGAAGAGCTTGCCCAGCGAGGCCTCTGAGCAGTATTTGACGAAAG; CCTCAGAACCAGATAAAAACCCCACAGCTGTGGAAGGACTGGGATCAGAGCCTGATAATTTGGTGATTAC; GTGGAAGCCCTTGAATGGTTTCGAATCTAATGGGCCAGGCCTTCAGTACAAAGTTAGCTGGCGCCAGAAA; GATGGTGATGATGAATGGACATCTGTGGTTGTGGCAAATGTATCCAAATATATTGTCTCAGGCACGCCAA; CCTTTGTTCCATACCTGATCAAAGTTCAGGCCCTGAATGACATGGGGTTTGCCCCCGAGCCAGCTGTAGT; CATGGGACATTCTGGAGAAGACCTCCCAATGGTGGCTCCTGGGAACGTGCGTGTGAATGTGGTGAACAGT; ACCTTAGCCGAGGTGCACTGGGACCCAGTACCTCTGAAAAGCATCCGAGGACACCTACAAGGCTATCGGA; TTTACTATTGGAAGACCCAGAGTTCATCTAAAAGAAACAGACGTCACATTGAGAAAAAGATCCTCACCTT; CCAAGGCAGCAAGACTCATGGCATGTTGCCGGGGCTAGAGCCCTTTAGCCACTACACACTGAATGTCCGA; GTGGTCAATGGGAAAGGGGAGGGCCCAGCCAGCCCTGACAGAGTCTTTAATACTCCAGAAGGAGTCCCCA; GTGCTCCCTCGTCTTTGAAGATTGTGAATCCAACACTGGACTCTCTCACTTTGGAATGGGATCCACCGAG; CCACCCGAATGGCATTTTGACAGAGTACACCTTAAAGTATCAGCCAATTAACAGCACACATGAATTAGGC; CCTCTGGTAGATTTGAAAATTCCTGCCAACAAGACACGGTGGACTTTAAAAAATTTAAATTTCAGCACTC; GATATAAGTTTTATTTCTATGCACAAACATCAGCAGGATCAGGAAGTCAAATTACAGAGGAAGCAGTAAC; AACTGTGGATGAAGCGATGGCAAGCCGGCAGGTGGATATTGCAACTCAGGGCTGGTTCATTGGTCTGATG; TGTGCTGTTGCTCTCCTTATCTTAATTTTGCTGATTGTTTGCTTCATCAGAAGAAACAAGGGTGGTAAAT; ATCCAGTTAAAGAAAAGGAAGATGCCCATGCTGACCCTGAAATCCAGCCTATGAAGGAAGATGATGGGAC; ATTTGGAGAATACAGTGATGCAGAAGACCACAAGCCTTTGAAAAAAGGAAGTCGAACTCCTTCAGACAGG; ACTGTGAAAAAAGAAGATAGTGACGACAGCCTAGTTGACTATGGAGAAGGGGTTAATGGCCAGTTCAATG; AGGATGGCTCCTTTATTGGACAATACAGTGGTAAGAAAGAGAAAGAGCCGGCTGAAGGAAACGAAAGCTC; AGAGGCACCTTCTCCTGTCAACGCCATGAATTCCTTTGTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230893 representing NM_001193584; Red=Cloning site Green=Tags(s); ; MQLKIMPKKKRLSAGRVPLILFLCQMISALEVPLDPKLLEDLVQPPTITQQSPKDYIIDPRENIVIQCEA; KGKPPPSFSWTRNGTHFDIDKDPLVTMKPGTGTLIINIMSEGKAETYEGVYQCTARNERGAAVSNNIVVR; PSRSPLWTKEKLEPITLQSGQSLVLPCRPPIGLPPPIIFWMDNSFQRLPQSERVSQGLNGDLYFSNVLPE; DTREDYICYARFNHTQTIQQKQPISVKVISAKSSRERPPTFLTPEGNASNKEELRGNVLSLECIAEGLPT; PIIYWAKEDGMLPKNRTVYKNFEKTLQIIHVSEADSGNYQCIAKNALGAIHHTISVRVKAAPYWITAPQN; LVLSPGEDGTLICRANGNPKPRISWLTNGVPIEIAPDDPSRKIDGDTIIFSNVQERSSAVYQCNASNEYG; YLLANAFVNVLAEPPRILTPANTLYQVIANRPALLDCAFFGSPLPTIEWFKGAKGSALHEDIYVLHENGT; LEIPVAQKDSTGTYTCVARNKLGMAKNEVHLEIKDATWIVKQPEYAVVQRGSMVSFECKVKHDHTLSLTV; LWLKDNRELPSDERFTVDKDHLVVADVSDDDSGTYTCVANTTLDSVSASAVLSVVAPTPTPAPVYDVPNP; PFDLELTDQLDKSVQLSWTPGDDNNSPITKFIIEYEDAMHKPGLWHHQTEVSGTQTTAQLKLSPYVNYSF; RVMAVNSIGKSLPSEASEQYLTKASEPDKNPTAVEGLGSEPDNLVITWKPLNGFESNGPGLQYKVSWRQK; DGDDEWTSVVVANVSKYIVSGTPTFVPYLIKVQALNDMGFAPEPAVVMGHSGEDLPMVAPGNVRVNVVNS; TLAEVHWDPVPLKSIRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGSKTHGMLPGLEPFSHYTLNVR; VVNGKGEGPASPDRVFNTPEGVPSAPSSLKIVNPTLDSLTLEWDPPSHPNGILTEYTLKYQPINSTHELG; PLVDLKIPANKTRWTLKNLNFSTRYKFYFYAQTSAGSGSQITEEAVTTVDEAMASRQVDIATQGWFIGLM; CAVALLILILLIVCFIRRNKGGKYPVKEKEDAHADPEIQPMKEDDGTFGEYSDAEDHKPLKKGSRTPSDR; TVKKEDSDDSLVDYGEGVNGQFNEDGSFIGQYSGKKEKEPAEGNESSEAPSPVNAMNSFV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193584
• ORF Size: 3540 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001193584.1, NP_001180513.1
• RefSeq ORF: 3543 bp
• Locus ID: 4897
• UniProt ID: Q92823
• Cytogenetics: 7q31.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 131.1 kDa
• Summary: Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]